Table 1

In vitro CTL responses in HHD mice immunized with recombinant lv-hTERT vector

PeptidesSequenceRelative avidityProtein of originCTL response
R/TPercentage specific lysis
p152 LLARCALPV Low hTERT 0/6 — 
pY152 YLARCALPV High hTERT 0/6 — 
p540 ILAKFLHWL High hTERT 5/6 64, 48, 57, 65, 41 
p555 ELLRSFFYV Low hTERT 0/6 — 
pY555 YLLRSFFYV High hTERT 1/6 16 
p572 RLFFYRKSV Low hTERT/mTERT 5/6 41, 42, 48, 52, 26 
pY572 YLFFYRKSV High hTERT/mTERT 6/6 48, 51, 64, 71, 38, 21 
p865 RLVDDFLLV High hTERT 3/6 54, 49, 33 
p988 DLQVNSLQTV Low hTERT/mTERT 6/6 46, 39, 48, 15, 19, 34 
pY988 YLQVNSLQTV High hTERT/mTERT 6/6 61, 44, 79, 27, 33, 52 
PeptidesSequenceRelative avidityProtein of originCTL response
R/TPercentage specific lysis
p152 LLARCALPV Low hTERT 0/6 — 
pY152 YLARCALPV High hTERT 0/6 — 
p540 ILAKFLHWL High hTERT 5/6 64, 48, 57, 65, 41 
p555 ELLRSFFYV Low hTERT 0/6 — 
pY555 YLLRSFFYV High hTERT 1/6 16 
p572 RLFFYRKSV Low hTERT/mTERT 5/6 41, 42, 48, 52, 26 
pY572 YLFFYRKSV High hTERT/mTERT 6/6 48, 51, 64, 71, 38, 21 
p865 RLVDDFLLV High hTERT 3/6 54, 49, 33 
p988 DLQVNSLQTV Low hTERT/mTERT 6/6 46, 39, 48, 15, 19, 34 
pY988 YLQVNSLQTV High hTERT/mTERT 6/6 61, 44, 79, 27, 33, 52 

The HLA-A*0201-restricted hTERT-derived peptides and their respective relative avidity have been already described.29-31  HHD mice were immunized subcutaneously with 2 × 107 TU of recombinant lv-hTERT vector, and their splenocytes were restimulated in vitro 10 days later with each peptide: p540 or p865, or with the pool of native and optimized form of indicated cryptic peptides (p152/pY152, p555/pY55, p572/pY572, and p988/pY988; 10 μg/mL). Peptide-specific CTLs were tested against RMAS/HHD cells pulsed or not with the corresponding peptide. Data represent the percentage of specific lysis of peptide-pulsed target cells at a 50:1 splenocyte/target ratio, after subtraction of nonspecific lysis of nonpulsed target cells (0%-8%).

CTL indicates cytotoxic T lymphocytes; HHD, HLA-A2.1 transgenic mice; R/T, number of responder/total number of immunized; and —, not applicable.

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