Table 1 Derivation and characteristics... | American Society of Hematology

Table 1

Derivation and characteristics of the anti-GIMAP monoclonal antibodies

GIMAP no.	MAb name	Rat γ isotype	Rat strain	♀/♂	Fusion partner	Immunogen	Vector/bacteria	Immunogen preparation
1	MAC420*	2a	LOU/C	♀	Y3Ag1.2.3†	GST-mGIMAP1aa1-238	pDEST15/RosettaDE3	Glutathione bead-eluted/gel-purified
4	MAC417‡	2b	LOU/C	♀	Y3Ag1.2.3	GST-mGIMAP4aa1-310	pGEX.4T1/Rosetta2	Glutathione bead-eluted
5	MAC421	2a	LEW	♀	Y3Ag1.2.3	GST-mGIMAP5aa1-270	pDEST15/RosettaDE3	Glutathione bead-eluted
6	MAC432	2b	DA	♂	IR983F§	GST-mGIMAP6 full length	pGEX.4T1/Rosetta	Inclusion bodies
9‖	MAC433	2a	DA	♂	Y3Ag1.2.3	GST-mGIMAP9 full length	pGEX.4T1/Rosetta	Inclusion bodies

GIMAP no.	MAb name	Rat γ isotype	Rat strain	♀/♂	Fusion partner	Immunogen	Vector/bacteria	Immunogen preparation
1	MAC420*	2a	LOU/C	♀	Y3Ag1.2.3†	GST-mGIMAP1aa1-238	pDEST15/RosettaDE3	Glutathione bead-eluted/gel-purified
4	MAC417‡	2b	LOU/C	♀	Y3Ag1.2.3	GST-mGIMAP4aa1-310	pGEX.4T1/Rosetta2	Glutathione bead-eluted
5	MAC421	2a	LEW	♀	Y3Ag1.2.3	GST-mGIMAP5aa1-270	pDEST15/RosettaDE3	Glutathione bead-eluted
6	MAC432	2b	DA	♂	IR983F§	GST-mGIMAP6 full length	pGEX.4T1/Rosetta	Inclusion bodies
9‖	MAC433	2a	DA	♂	Y3Ag1.2.3	GST-mGIMAP9 full length	pGEX.4T1/Rosetta	Inclusion bodies

See Saunders et al.⁴

†

See Galfrè et al.²¹

‡

See Carter et al.²²

See Bazin.²³

‖

This denotes the mGIMAP polypeptide whose predicted N-terminal aa sequence is MAEPS, not the second of the 2 hGIMAP7-like polypeptides in mice which begins MAGQG.

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