Sequence of primers and PCR conditions
| mRNA . | Primer . | Accession no. . | Expected size, bp . | Annealing temp, °C . | PCR cycles . |
|---|---|---|---|---|---|
| GAPDH | F: ACCACAGTCCATGCCATCAC | AF261085 | 446 | 60 | 32 |
| R: TCCACCACCCTGTTGCTGTA | |||||
| Fyn | F: GAATTTAGATAATGGGCTGTGTGCAATGTAAGGAT | NM_153047 | 1605 | 60 | 34 |
| R: ACCCGGTTACAGGTTTTCACCAGGT | |||||
| C-mip | F: GGCCATGGATGTGACCAGCAGCTC | AK096598 | 2330 | 60 | 34 |
| R: TGGGAGCTTCACCAGGCTTCGGTGTAGC | |||||
| FAT | F: CAGCGACTCCATCCAGAAGCCTAGCTG | NM_005245 | 450 | 60 | 35 |
| R: CGAGGCATGTCTCTAGGAGGGTGGATG | |||||
| Neuropilin-1 | F: CTGATTCAGGCTCCGGACCCATACCAGAG | BC007737 | 684 | 60 | 35 |
| R: CTCCTGATTCCATGCCCAGAGCTTCCA |
| mRNA . | Primer . | Accession no. . | Expected size, bp . | Annealing temp, °C . | PCR cycles . |
|---|---|---|---|---|---|
| GAPDH | F: ACCACAGTCCATGCCATCAC | AF261085 | 446 | 60 | 32 |
| R: TCCACCACCCTGTTGCTGTA | |||||
| Fyn | F: GAATTTAGATAATGGGCTGTGTGCAATGTAAGGAT | NM_153047 | 1605 | 60 | 34 |
| R: ACCCGGTTACAGGTTTTCACCAGGT | |||||
| C-mip | F: GGCCATGGATGTGACCAGCAGCTC | AK096598 | 2330 | 60 | 34 |
| R: TGGGAGCTTCACCAGGCTTCGGTGTAGC | |||||
| FAT | F: CAGCGACTCCATCCAGAAGCCTAGCTG | NM_005245 | 450 | 60 | 35 |
| R: CGAGGCATGTCTCTAGGAGGGTGGATG | |||||
| Neuropilin-1 | F: CTGATTCAGGCTCCGGACCCATACCAGAG | BC007737 | 684 | 60 | 35 |
| R: CTCCTGATTCCATGCCCAGAGCTTCCA |
F indicates forward; R, reverse; and bp, base pair.