Primers used for real-time qPCR
| Gene ID . | Gene name . | Sequence . |
|---|---|---|
| il6 | IL-6 | 5′-TACCACTTCACAAGTCGGAGGC-3′; 5′-CTGCAAGTGCATCATCGTTGTTC-3′ |
| tnf alpha | TNFa | 5′-GGTGCCTATGTCTCAGCCTCTT-3′; 5′-GCCATAGAACTGATGAGAGGGAG-3′ |
| il 12b | IL-12(p40) | 5′-TTGAACTGGCGTTGGAAGCACG-3′; 5′-CCACCTGTGAGTTCTTCAAAGGC-3′ |
| gapdh | GAPDH | 5′-ATT GTG GAA GGG CTC ATG AC-3′; 5′-AGT GGA TGC AGG GAT GAT GT-3′ |
| Gene ID . | Gene name . | Sequence . |
|---|---|---|
| il6 | IL-6 | 5′-TACCACTTCACAAGTCGGAGGC-3′; 5′-CTGCAAGTGCATCATCGTTGTTC-3′ |
| tnf alpha | TNFa | 5′-GGTGCCTATGTCTCAGCCTCTT-3′; 5′-GCCATAGAACTGATGAGAGGGAG-3′ |
| il 12b | IL-12(p40) | 5′-TTGAACTGGCGTTGGAAGCACG-3′; 5′-CCACCTGTGAGTTCTTCAAAGGC-3′ |
| gapdh | GAPDH | 5′-ATT GTG GAA GGG CTC ATG AC-3′; 5′-AGT GGA TGC AGG GAT GAT GT-3′ |
For each pair of primers, the forward sequence is listed first, followed by the reverse sequence.
qPCR indicates quantitative polymerase chain reaction; IL, interleukin; TNF, tumor necrosis factor, and GAPDH, glyceraldehyde-3-phosphate dehydrogenase.