T-ALLs from mixed CG1 backgrounds can be introduced into nonirradiated CG1 recipients
| Tumor ID . | Donor background . | No. of transplanted cells . | Recipient background . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| AB + IR . | CG1 . | CG1 × Alb . | AB noIR . | AB/CG1 . | AB/CG1 × CG1 . | [AB/CGi × CG1] × CG1 . | |||
| 1 | CG1 | 5 × 105 | 5 of 5 | 7 of 7 | 7 of 7 | 0 of 6 | NA | NA | NA |
| 3 | CG1 | 5 × 104 | 3 of 4 | 6 of 6 | 3 of 9 | 0 of 7 | NA | NA | NA |
| 4 | CG1 | 5 × 105 | 2 of 3 | 14 of 14 | 14 of 15 | 0 of 2 | NA | NA | NA |
| Mixed* | CG1 | 5 × 105 | NA | NA | NA | NA | 1 of 23 | 8 of 19 | 18 of 18 |
| A | CG1/Alb | 5 × 105 | 1 of 1 | 0 of 7 | 0 of 9 | 0 of 6 | NA | NA | NA |
| B | CG1/Alb | 5 × 105 | 2 of 5 | 0 of 7 | 0 of 6 | 0 of 7 | NA | NA | NA |
| C | CG1/Alb | 5 × 105 | 2 of 2 | 0 of 4 | 0 of 6 | 0 of 5 | NA | NA | NA |
| D.1 | CG1/Alb × CG1 | 5 × 105 | NA | 0 of 13 | NA | NA | NA | NA | NA |
| D.2 | CG1/Alb × CG1 | 5 × 105 | NA | 0 of 13 | NA | NA | NA | NA | NA |
| D.3 | CG1/AIb × CG1 | 5 × 105 | NA | 0 of 11 | NA | NA | NA | NA | NA |
| D.4 | CG1/AIb × CG1 | 5 × 105 | NA | 0 of 11 | NA | NA | NA | NA | NA |
| D.5 | CG1/AIb × CG1 | 5 × 105 | NA | 11 of 12 | NA | NA | NA | NA | NA |
| D.6 | CG1/Alb × CG1 | 5 × 105 | NA | 11 of 11 | NA | NA | NA | NA | NA |
| Tumor ID . | Donor background . | No. of transplanted cells . | Recipient background . | ||||||
|---|---|---|---|---|---|---|---|---|---|
| AB + IR . | CG1 . | CG1 × Alb . | AB noIR . | AB/CG1 . | AB/CG1 × CG1 . | [AB/CGi × CG1] × CG1 . | |||
| 1 | CG1 | 5 × 105 | 5 of 5 | 7 of 7 | 7 of 7 | 0 of 6 | NA | NA | NA |
| 3 | CG1 | 5 × 104 | 3 of 4 | 6 of 6 | 3 of 9 | 0 of 7 | NA | NA | NA |
| 4 | CG1 | 5 × 105 | 2 of 3 | 14 of 14 | 14 of 15 | 0 of 2 | NA | NA | NA |
| Mixed* | CG1 | 5 × 105 | NA | NA | NA | NA | 1 of 23 | 8 of 19 | 18 of 18 |
| A | CG1/Alb | 5 × 105 | 1 of 1 | 0 of 7 | 0 of 9 | 0 of 6 | NA | NA | NA |
| B | CG1/Alb | 5 × 105 | 2 of 5 | 0 of 7 | 0 of 6 | 0 of 7 | NA | NA | NA |
| C | CG1/Alb | 5 × 105 | 2 of 2 | 0 of 4 | 0 of 6 | 0 of 5 | NA | NA | NA |
| D.1 | CG1/Alb × CG1 | 5 × 105 | NA | 0 of 13 | NA | NA | NA | NA | NA |
| D.2 | CG1/Alb × CG1 | 5 × 105 | NA | 0 of 13 | NA | NA | NA | NA | NA |
| D.3 | CG1/AIb × CG1 | 5 × 105 | NA | 0 of 11 | NA | NA | NA | NA | NA |
| D.4 | CG1/AIb × CG1 | 5 × 105 | NA | 0 of 11 | NA | NA | NA | NA | NA |
| D.5 | CG1/AIb × CG1 | 5 × 105 | NA | 11 of 12 | NA | NA | NA | NA | NA |
| D.6 | CG1/Alb × CG1 | 5 × 105 | NA | 11 of 11 | NA | NA | NA | NA | NA |
The rag2-mMyc and rag2-GFP, rag2-dsREDexpress, or rag2-zsYellow transgenes were coinjected into various genetic backgrounds at the one-cell stage of life, and T-ALLs that developed were introduced into recipient animals by intraperitoneal injection. Transplanted animals were scored for T-ALL engraftment at 10, 20, and 30 days. Table is a compilation of the total number of animals that had engrafted disease by 30 days.
AB indicates AB strain; Alb, albino; IR, irradiation; and NA, not assayed.
Mixed is a combination of 3 independent experiments in which T-ALLs arising in CG1 animals were introduced into AB-strain fish that were once outcrossed to CG1-strain animals (AB/CG1), twice outcrossed (AB/CG1 × CG1), or 3 times outcrossed ([AB/CG1 × CG1] × CG1).