Table 1

Comparison of protein expression in reticulocytes and erythrocytes

Cytoskeletal proteinsErythrocyte percentage of reticulocyte (intact cells, n)Erythrocyte percentage of reticulocyte (ghosts, n)Transmembrane proteinsErythrocyte percentage of reticulocyte (ghosts, n)
Actin 0.58 ± 0.01* (4) 0.98 ± 0.06 (3) CD71 0.14 ± 0.12* (3) 
Myosin 0.54 ± 0.16 (3) 0.47 ± 0.02* (3) GLUT4 0.21 ± 0.15* (3) 
Tropomyosin 0.30 ± 0.18 (3) 0.40 ± 0.04* (3) Na+-K+ ATPase α 0.26 ± 0.01* (3) 
Adducin 0.74 ± 0.09 (4) 1.03 ± 0.13 (3) LW 0.11 ± 0.08* (3) 
α-Spectrin 0.95 ± 0.09 (4) 0.93 ± 0.07 (3) NHE1 0.53 ± 0.31 (4) 
β-Spectrin 1.02 ± 0.12 (4) 1.01 ± 0.15 (3) Duffy 0.71 ± 0.12 (4) 
Ankyrin 0.93 ± 0.11 (4) 0.91 ± 0.13 (3) Kell 0.38 ± 0.19* (4) 
4.1R 0.98 ± 0.00 (4) 0.97 ± 0.15 (3) GPA 0.61 ± 0.19 (3) 
4.2 1.03 ± 0.24 (3) 1.08 ± 0.16 (3) CD47 0.70 ± 0.04* (4) 
4.9 (Dematin) 0.91 ± 0.09 (4) 0.87 ± 0.13 (3) Band3 1.12 ± 0.09 (3) 
Tropomodulin 1.04 ± 0.05 (4) 1.09 ± 0.06 (3) Rh 1.26 ± 0.05* (4) 
p55 0.87 ± 0.10 (3) 0.92 ± 0.14 (3) RhAG 1.26 ± 0.11* (4) 
   XK 1.35 ± 0.20 (4) 
   GPC 1.31 ± 0.20 (4) 
Cytoskeletal proteinsErythrocyte percentage of reticulocyte (intact cells, n)Erythrocyte percentage of reticulocyte (ghosts, n)Transmembrane proteinsErythrocyte percentage of reticulocyte (ghosts, n)
Actin 0.58 ± 0.01* (4) 0.98 ± 0.06 (3) CD71 0.14 ± 0.12* (3) 
Myosin 0.54 ± 0.16 (3) 0.47 ± 0.02* (3) GLUT4 0.21 ± 0.15* (3) 
Tropomyosin 0.30 ± 0.18 (3) 0.40 ± 0.04* (3) Na+-K+ ATPase α 0.26 ± 0.01* (3) 
Adducin 0.74 ± 0.09 (4) 1.03 ± 0.13 (3) LW 0.11 ± 0.08* (3) 
α-Spectrin 0.95 ± 0.09 (4) 0.93 ± 0.07 (3) NHE1 0.53 ± 0.31 (4) 
β-Spectrin 1.02 ± 0.12 (4) 1.01 ± 0.15 (3) Duffy 0.71 ± 0.12 (4) 
Ankyrin 0.93 ± 0.11 (4) 0.91 ± 0.13 (3) Kell 0.38 ± 0.19* (4) 
4.1R 0.98 ± 0.00 (4) 0.97 ± 0.15 (3) GPA 0.61 ± 0.19 (3) 
4.2 1.03 ± 0.24 (3) 1.08 ± 0.16 (3) CD47 0.70 ± 0.04* (4) 
4.9 (Dematin) 0.91 ± 0.09 (4) 0.87 ± 0.13 (3) Band3 1.12 ± 0.09 (3) 
Tropomodulin 1.04 ± 0.05 (4) 1.09 ± 0.06 (3) Rh 1.26 ± 0.05* (4) 
p55 0.87 ± 0.10 (3) 0.92 ± 0.14 (3) RhAG 1.26 ± 0.11* (4) 
   XK 1.35 ± 0.20 (4) 
   GPC 1.31 ± 0.20 (4) 

Note that equivalent amounts of cholesterol were loaded.

GPA indicates glycophorin A; GPC, glycophorin C; NHE1, sodium/hydrogen exchanger 1; and RhAG, Rh-associated glycoprotein.

*

P ≤ .005.

P ≤ .01.

P ≤ .05.

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