The AML sample is indicated in the first column and the subtype in the second. Some samples were tested in more than one strain of mouse (indicated by the lowercase Roman numeral). NSG indicates that NOD/SCID/IL2rγ^−/− mice were used in the experiment; B2m, NOD/SCID/β₂m^−/− mice; NT, not tested; WT2, wild-type NPM AML sample 2; and NA, not applicable as this sample lacks a CD34⁺CD38⁻ population. The figures in parentheses indicate the mean percentage of AML in the mouse bone marrow.

The LICs from sample 1 are restricted to the CD34⁻ fraction (Table 3).

Normal multilineage engraftment observed from the CD34⁺CD38⁻ fraction from one or more mice. The DNA from the grafts contained wild-type NPM.

The graft was assessed by quantitative PCR and shown to contain mutated NPM.

LICs are not found in the CD34^dim and CD34⁺ fractions that include all the CD34⁺CD38⁺ and CD34⁺CD38⁻ cells (Table 3).

Both CD45⁺CD33⁺ and CD45⁺CD19⁺ populations were observed in some mice transplanted with CD34⁺CD38⁻ cells. The CD19⁺ cells contain mostly wild-type NPM, whereas the CD33⁺ fractions contain mostly mutant NPM, consistent with the presence of LICs and normal repopulating cells in the CD34⁺CD38⁻ fraction. Some of the data from the CD34⁺CD38⁺ and CD34⁺CD38⁻ fractions of samples 15 and 18 have been previously published.³