Characteristics of CLL mAbs
| CLL no. . | Subset . | Mutation status . | % mutated . | IGHV . | HCDR3 . | IGH, GenBank accession no. . | IGKV/IGLV . | IGK/IGL, GenBank accession no. . | MFIR . |
|---|---|---|---|---|---|---|---|---|---|
| 169 | — | M | 9.0 | 3-33 | CAREGGVTGQGGFDYW | AY055480 | K4-01 | FJ039794 | 0.85 |
| 255 | — | M | 4.2 | 4-59 | CARHRGYESSGYYSSYFDYW | FJ039782 | L3-01 | FJ039804 | 1.05 |
| 141 | — | U | 0.0 | 4-34 | CARGDWRIVVVPAAVDTAMAANWFDPW | AF022005 | K1-27 | AY043160 | 1.10 |
| 260 | — | U | 0.0 | 4-b | CARAEIVVVPAAYYYYYGMDVW | FJ039783 | K1-27 | FJ039796 | 2.86 |
| 376 | — | U | 0.0 | 1-24 | CATSAFTVTHAEYFQHW | FJ039787 | K3-11 | FJ039798 | 0.89 |
| 415 | — | U | 0.0 | 1-03 | CARRPESGYSFVTPFDYW | FJ039788 | K1D-39 | FJ039799 | 1.95 |
| 562 | — | U | 1.7 | 3-21 | CVRDEITVAATRPCPW | FJ039789 | L3-21 | FJ039805 | 1.09 |
| 282 | 2 | M | 2.4 | 3-21 | CARDANGMDVW | AY553643 | L3-21 | AY574941 | 0.99 |
| 412 | 2 | U | 1.8 | 3-21 | CARDQNGMDVW | AY553648 | L3-21 | AY574946 | 1.15 |
| 183 | 4 | M | 3.2 | 4-34 | CARGYGDTPTIRRYYYYGMDVW | AF021948 | K2-30 | AY043097 | 0.74 |
| 240 | 4 | M | 3.2 | 4-34 | CARGYADTPVFRRYYYYGMDVW | AY553641 | K2-30 | AY574937 | 1.01 |
| 342 | 4 | M | 2.8 | 4-34 | CARGWGDTPMLKRYYYYGLDVW | AY553646 | K2-30 | AY574944 | 0.72 |
| 154 | 1 | M | 2.1 | 1-18 | CAREQWLVLSHFDYW | AF022009 | K1D-39 | AY043164 | 5.11 |
| 270 | 1 | U | 0.0 | 1-02 | CARVQWLGLRHFDYW | AY055487 | K1D-39 | AY574940 | 2.40 |
| 340 | 1 | U | 0.0 | 1-02 | CAREQWLVLKNFDYW | AY553645 | K1D-39 | AY574943 | 3.99 |
| 360 | 1 | U | 0.0 | 1-03 | CAREQWLVLNYFDYW | AY553647 | K1D-39 | AY574945 | 4.17 |
| 068 | 6 | U | 0.0 | 1-69 | CARGGDYDYVWGSYRSNDAFDIW | AY553640 | K3-20 | AY574935 | 3.82 |
| 258 | 6 | U | 0.0 | 1-69 | CARGGIYDYVWGSYRPNDAFDIW | AY055485 | K3-20 | AY574938 | 2.07 |
| 114 | 8 | U | 0.0 | 4-39 | CARRFGYSSSWYGLDWFDPW | AY268372 | K1D-39 | AY043094 | 17.05 |
| 657 | 8 | U | 0.3 | 4-39 | CASKTGYSSSWYGRDWFDPW | FJ039790 | K1D-39 | FJ039800 | 23.90 |
| 845 | 8 | U | 0.0 | 4-39 | CASSTGYSSSWYSPTNWFDPW | FJ039791 | K1D-39 | FJ039801 | 25.25 |
| 014 | 9 | U | 0.0 | 1-69 | CATKNDFWSGYYEGYYYYYYMDVW | AF021951 | L3-01 | AY043106 | 1.84 |
| 246 | 9 | U | 0.3 | 1-69 | CARSDQNYDFWSGYFRYYGMDVW | FJ039781 | K3-20 | FJ039795 | 9.53 |
| 355 | 9 | U | 0.0 | 1-69 | CARADLPYYDFWSGMYYYGMDVW | FJ039784 | K1-05 | AJ697904 | 22.55 |
| 366 | 9 | U | 0.0 | 3-21 | CARGVLNYDFWSVYYYYGMDVW | FJ039786 | K4-01 | FJ039797 | 3.56 |
| DO13 | 28 | U | 0.0 | 1-02 | CARQFSGSPTRYYYYYGMDVW | FJ039793 | K4-01 | FJ039803 | 10.08 |
| CLL no. . | Subset . | Mutation status . | % mutated . | IGHV . | HCDR3 . | IGH, GenBank accession no. . | IGKV/IGLV . | IGK/IGL, GenBank accession no. . | MFIR . |
|---|---|---|---|---|---|---|---|---|---|
| 169 | — | M | 9.0 | 3-33 | CAREGGVTGQGGFDYW | AY055480 | K4-01 | FJ039794 | 0.85 |
| 255 | — | M | 4.2 | 4-59 | CARHRGYESSGYYSSYFDYW | FJ039782 | L3-01 | FJ039804 | 1.05 |
| 141 | — | U | 0.0 | 4-34 | CARGDWRIVVVPAAVDTAMAANWFDPW | AF022005 | K1-27 | AY043160 | 1.10 |
| 260 | — | U | 0.0 | 4-b | CARAEIVVVPAAYYYYYGMDVW | FJ039783 | K1-27 | FJ039796 | 2.86 |
| 376 | — | U | 0.0 | 1-24 | CATSAFTVTHAEYFQHW | FJ039787 | K3-11 | FJ039798 | 0.89 |
| 415 | — | U | 0.0 | 1-03 | CARRPESGYSFVTPFDYW | FJ039788 | K1D-39 | FJ039799 | 1.95 |
| 562 | — | U | 1.7 | 3-21 | CVRDEITVAATRPCPW | FJ039789 | L3-21 | FJ039805 | 1.09 |
| 282 | 2 | M | 2.4 | 3-21 | CARDANGMDVW | AY553643 | L3-21 | AY574941 | 0.99 |
| 412 | 2 | U | 1.8 | 3-21 | CARDQNGMDVW | AY553648 | L3-21 | AY574946 | 1.15 |
| 183 | 4 | M | 3.2 | 4-34 | CARGYGDTPTIRRYYYYGMDVW | AF021948 | K2-30 | AY043097 | 0.74 |
| 240 | 4 | M | 3.2 | 4-34 | CARGYADTPVFRRYYYYGMDVW | AY553641 | K2-30 | AY574937 | 1.01 |
| 342 | 4 | M | 2.8 | 4-34 | CARGWGDTPMLKRYYYYGLDVW | AY553646 | K2-30 | AY574944 | 0.72 |
| 154 | 1 | M | 2.1 | 1-18 | CAREQWLVLSHFDYW | AF022009 | K1D-39 | AY043164 | 5.11 |
| 270 | 1 | U | 0.0 | 1-02 | CARVQWLGLRHFDYW | AY055487 | K1D-39 | AY574940 | 2.40 |
| 340 | 1 | U | 0.0 | 1-02 | CAREQWLVLKNFDYW | AY553645 | K1D-39 | AY574943 | 3.99 |
| 360 | 1 | U | 0.0 | 1-03 | CAREQWLVLNYFDYW | AY553647 | K1D-39 | AY574945 | 4.17 |
| 068 | 6 | U | 0.0 | 1-69 | CARGGDYDYVWGSYRSNDAFDIW | AY553640 | K3-20 | AY574935 | 3.82 |
| 258 | 6 | U | 0.0 | 1-69 | CARGGIYDYVWGSYRPNDAFDIW | AY055485 | K3-20 | AY574938 | 2.07 |
| 114 | 8 | U | 0.0 | 4-39 | CARRFGYSSSWYGLDWFDPW | AY268372 | K1D-39 | AY043094 | 17.05 |
| 657 | 8 | U | 0.3 | 4-39 | CASKTGYSSSWYGRDWFDPW | FJ039790 | K1D-39 | FJ039800 | 23.90 |
| 845 | 8 | U | 0.0 | 4-39 | CASSTGYSSSWYSPTNWFDPW | FJ039791 | K1D-39 | FJ039801 | 25.25 |
| 014 | 9 | U | 0.0 | 1-69 | CATKNDFWSGYYEGYYYYYYMDVW | AF021951 | L3-01 | AY043106 | 1.84 |
| 246 | 9 | U | 0.3 | 1-69 | CARSDQNYDFWSGYFRYYGMDVW | FJ039781 | K3-20 | FJ039795 | 9.53 |
| 355 | 9 | U | 0.0 | 1-69 | CARADLPYYDFWSGMYYYGMDVW | FJ039784 | K1-05 | AJ697904 | 22.55 |
| 366 | 9 | U | 0.0 | 3-21 | CARGVLNYDFWSVYYYYGMDVW | FJ039786 | K4-01 | FJ039797 | 3.56 |
| DO13 | 28 | U | 0.0 | 1-02 | CARQFSGSPTRYYYYYGMDVW | FJ039793 | K4-01 | FJ039803 | 10.08 |
Subset number or — indicates not attributable to a defined subset.4,13,24 U indicates unmutated; and M, more than 2% mutated compared with germline according to IMGT.25
IGHV mutation percentage compared with germline according to IMGT.25 IGHV, IGKV (K), and IGLV (L) gene name is according to IMGT nomenclature.25
HCDR3 amino acid sequence is shown in single-letter code plus junctional C and W residues according to IMGT.25
MFIR measurement of MEAC binding from Figure 3C.