Table 1 Homeostatic proliferation of... | American Society of Hematology

Table 1

Homeostatic proliferation of Des-TCR CD8 T cells does not break tolerance

Donor mouse		Incidence of tumor growth (P815.K^b.B7)
1	No cell transfer	14 of 14
2	Des-TCR.RAG2^−/−	0 of 7
3	Des-TCR.RAG2^−/− (Tx)	2 of 7
4	Des-TCR × 2.4KerIV-K^b.RAG2^−/− (Tx)	14 of 15

Donor mouse		Incidence of tumor growth (P815.K^b.B7)
1	No cell transfer	14 of 14
2	Des-TCR.RAG2^−/−	0 of 7
3	Des-TCR.RAG2^−/− (Tx)	2 of 7
4	Des-TCR × 2.4KerIV-K^b.RAG2^−/− (Tx)	14 of 15

Thirty thousand MACS-purified Des-TCR CD8 T cells from reactive Des-TCR.RAG2^−/−, thymectomized reactive Des-TCR.RAG2^−/−, and thymectomized tolerant Des-TCR × 2.4KerIV-K^b.RAG2^−/− donor mice were transferred intravenously into RAG2^−/− mice. After 3 days the mice were challenged subcutaneously with 2 × 10⁵ P815.K^b.B7 tumor cells and the mice were then analyzed for tumor growth. After 14 to 18 days, most tumor-bearing mice had to be sacrificed due to the size of the tumors. Tumor-free mice were continued to be observed. The summarized data are from 3 independent experiments.

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