Clonal analysis of lymphomas by T-cell receptor rearrangement patterns
| . | Mouse strain* . | Mouse no. . | Virus . | Clonality† . | Major rearrangements . |
|---|---|---|---|---|---|
| Tissue ID‡ | |||||
| 7762 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | BV15-BJ1.6, BV27-BJ1.1-1.2, BV12-BJ1.1-1.2 |
| 7762 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | ND |
| 7762 | NOD/SCID | 5 | HTLV-1 | Oligoclonal | ND |
| 7241 | NOD/SCID | 2 | HTLV-1 | Oligoclonal | ND |
| 7241 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | ND |
| 1594 | NSG | 4 | HTLV-1 | Monoclonal | BV05-BV2.6 |
| 1594 | NSG | 5 | HTLV-1 | Oligoclonal | BV05-J1.5, BV11-BJ1.5, BV24-BJ2.3 |
| 9321 | NSG | 2 | HTLV-1 | Oligoclonal | ND |
| 7749 | NOD/SCID | 3 | LV-Tax1 | Monoclonal | BV12-BJ1.1-1.2 |
| 7749 | NOD/SCID | 4 | LV-Tax1 | Monoclonal | BV12-BJ1.1-1.3 |
| Controls | |||||
| Hu-NSG (spleen)§ | NSG | Mock | Polyclonal | ||
| RV-ATL‖ | NA | Monoclonal | BV29-BJ2.2 | ||
| SLB-1 cell line¶ | NA | Monoclonal | BV15-BJ1.6, BV27-BJ1.1-1.2 |
| . | Mouse strain* . | Mouse no. . | Virus . | Clonality† . | Major rearrangements . |
|---|---|---|---|---|---|
| Tissue ID‡ | |||||
| 7762 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | BV15-BJ1.6, BV27-BJ1.1-1.2, BV12-BJ1.1-1.2 |
| 7762 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | ND |
| 7762 | NOD/SCID | 5 | HTLV-1 | Oligoclonal | ND |
| 7241 | NOD/SCID | 2 | HTLV-1 | Oligoclonal | ND |
| 7241 | NOD/SCID | 1 | HTLV-1 | Oligoclonal | ND |
| 1594 | NSG | 4 | HTLV-1 | Monoclonal | BV05-BV2.6 |
| 1594 | NSG | 5 | HTLV-1 | Oligoclonal | BV05-J1.5, BV11-BJ1.5, BV24-BJ2.3 |
| 9321 | NSG | 2 | HTLV-1 | Oligoclonal | ND |
| 7749 | NOD/SCID | 3 | LV-Tax1 | Monoclonal | BV12-BJ1.1-1.2 |
| 7749 | NOD/SCID | 4 | LV-Tax1 | Monoclonal | BV12-BJ1.1-1.3 |
| Controls | |||||
| Hu-NSG (spleen)§ | NSG | Mock | Polyclonal | ||
| RV-ATL‖ | NA | Monoclonal | BV29-BJ2.2 | ||
| SLB-1 cell line¶ | NA | Monoclonal | BV15-BJ1.6, BV27-BJ1.1-1.2 |
HPCs indicate hematopoietic progenitor cells; HTLV-1, human T-lymphotropic virus type-1; LV, lentiviral vector; NA, not applicable; ND, no data available; NOD/SCID, nonobese diabetic severe combined immunodeficiency; and NSG, NOD/SCID IL-2γ chainnull.
NOD/SCID mice were inoculated intravenously either with HTLV-1–infected or LV-Tax–transduced CD34+ HPCs, as described in Table 1. NSG mice were inoculated with HTLV-1–infected CD34+ HPCs at 1-2 days of age, intrahepatically.
DNA from lymphomas from HTLV-1-HU-NOD/SCID, HTLV-1-HU-NSG, and LV-Tax1-HU-NOD/SCID mice were analyzed for combinatorial diversity of the human T-cell receptor β-chain. Samples demonstrating 1-2 predominant rearrangements are designated as monoclonal, 3-50 rearrangements as oligoclonal, and > 50 rearrangements as polyclonal.
ID for corresponding fetal liver donor tissue as the source for CD34+ HPCs as described in Table 1.
Spleen of a HU-NSG mice reconstituted with CD34+ HPCs derived from donor matched fetal liver tissue.
ATL cell line serially propagated in NOD/SCID mice.28
HTLV-1 in vitro–transformed cell line.