Summary of DsRed+ cells after differentiation of SB100X-transfected CD34+ HPCs into lymphoid and myeloid lineages in vitro
| . | % of DsRed+ cells (> 3 weeks after transfection) . | ||
|---|---|---|---|
| Mock . | Transposon alone . | Transposon + SB100X . | |
| T cells | |||
| 1* | 0.80 | 0.37 | 19.37 |
| 2 | ND | ND | 6.23 |
| 3 | 0.12 | 0.26 | 3.17 |
| 4 | ND | ND | 21.84 |
| 5 | 0.28 | 0.40 | 5.67 |
| 6 | ND | ND | 7.97 |
| 7 | ND | ND | 13.80 |
| Median | 0.28 | 0.37 | 7.97 |
| B cells | |||
| 1 | 0.22 | 0.12 | 5.80 |
| 2 | ND | ND | 9.88 |
| 3 | 0.10 | 0.03 | 3.83 |
| 4 | ND | ND | 18.66 |
| Median | 0.22 | 0.12 | 7.84 |
| NK cells | |||
| 1 | 0.21 | 0.27 | 13.11 |
| 2 | ND | ND | 3.53 |
| 3 | 0.15 | 0.17 | 5.72 |
| 4 | ND | ND | 79.98 |
| 5 | 0.08 | 0.01 | 7.88 |
| Median | 0.15 | 0.17 | 7.88 |
| Myeloid cells | |||
| 1 | 0.14 | 0.61 | 11.37 |
| 2 | ND | ND | 6.11 |
| 3 | 0.39 | 0.19 | 15.63 |
| 4 | ND | ND | 7.59 |
| Median | 0.27 | 0.4 | 9.48 |
| . | % of DsRed+ cells (> 3 weeks after transfection) . | ||
|---|---|---|---|
| Mock . | Transposon alone . | Transposon + SB100X . | |
| T cells | |||
| 1* | 0.80 | 0.37 | 19.37 |
| 2 | ND | ND | 6.23 |
| 3 | 0.12 | 0.26 | 3.17 |
| 4 | ND | ND | 21.84 |
| 5 | 0.28 | 0.40 | 5.67 |
| 6 | ND | ND | 7.97 |
| 7 | ND | ND | 13.80 |
| Median | 0.28 | 0.37 | 7.97 |
| B cells | |||
| 1 | 0.22 | 0.12 | 5.80 |
| 2 | ND | ND | 9.88 |
| 3 | 0.10 | 0.03 | 3.83 |
| 4 | ND | ND | 18.66 |
| Median | 0.22 | 0.12 | 7.84 |
| NK cells | |||
| 1 | 0.21 | 0.27 | 13.11 |
| 2 | ND | ND | 3.53 |
| 3 | 0.15 | 0.17 | 5.72 |
| 4 | ND | ND | 79.98 |
| 5 | 0.08 | 0.01 | 7.88 |
| Median | 0.15 | 0.17 | 7.88 |
| Myeloid cells | |||
| 1 | 0.14 | 0.61 | 11.37 |
| 2 | ND | ND | 6.11 |
| 3 | 0.39 | 0.19 | 15.63 |
| 4 | ND | ND | 7.59 |
| Median | 0.27 | 0.4 | 9.48 |
ND indicates not done.
Number of experiments.