Annexin V positivity of T lymphocytes following incubation with PAS
| . | PAS concentration (μM) . | |||
|---|---|---|---|---|
| 5 . | 10 . | 20 . | 40 . | |
| CD3+/CD4+ cells | ||||
| Donor 1 | 0.2 | −0.3 | 10.9 | 23.3 |
| Donor 2 | −2.8 | −3.1 | 14.7 | 28.1 |
| Donor 3 | −2.2 | −0.2 | 13.3 | 39.7 |
| CD3+/CD8+ cells | ||||
| Donor 1 | −0.5 | −2.7 | 5.6 | 16.7 |
| Donor 2 | −2.7 | −1.8 | 8.8 | 19.7 |
| Donor 3 | 0.2 | 1.1 | 8.5 | 29.8 |
| . | PAS concentration (μM) . | |||
|---|---|---|---|---|
| 5 . | 10 . | 20 . | 40 . | |
| CD3+/CD4+ cells | ||||
| Donor 1 | 0.2 | −0.3 | 10.9 | 23.3 |
| Donor 2 | −2.8 | −3.1 | 14.7 | 28.1 |
| Donor 3 | −2.2 | −0.2 | 13.3 | 39.7 |
| CD3+/CD8+ cells | ||||
| Donor 1 | −0.5 | −2.7 | 5.6 | 16.7 |
| Donor 2 | −2.7 | −1.8 | 8.8 | 19.7 |
| Donor 3 | 0.2 | 1.1 | 8.5 | 29.8 |
Peripheral blood mononuclear cells from 3 normal donors were incubated with increasing concentrations of PAS. The percentage of annexin V–positive cells within the CD3+/CD4+ or CD3+/CD8+ populations was determined as described in “Quantitation of annexin V–positive CLL cells or T lymphocyte subsets.” The PAS-specific increase in annexin V positivity relative to control cells was computed using an established equation.32