qRT-PCR for Noxa mRNA after treatment with PAS
| Patient no. . | Fold increase in Noxa mRNA . |
|---|---|
| AS2 | 0.85 |
| AS6 | 5.90 |
| AS7 | 4.66 |
| AS10 | 5.70 |
| AS19 | 6.45 |
| AS20 | 2.71 |
| AS21 | 0.75 |
| AS22 | 5.21 |
| AS27 | 4.92 |
| AS29 | 1.53 |
| Median | 4.79 |
| Mean | 3.87 |
| Patient no. . | Fold increase in Noxa mRNA . |
|---|---|
| AS2 | 0.85 |
| AS6 | 5.90 |
| AS7 | 4.66 |
| AS10 | 5.70 |
| AS19 | 6.45 |
| AS20 | 2.71 |
| AS21 | 0.75 |
| AS22 | 5.21 |
| AS27 | 4.92 |
| AS29 | 1.53 |
| Median | 4.79 |
| Mean | 3.87 |
Noxa expression was assessed on a LightCycler 2, with actin used as the control gene. A plot of the fold increase in Noxa transcripts versus the IC50 determined for the same isolate yielded a correlation coefficient of 0.66.