Organ pathology in GbG mice that underwent transplantation after reduced-intensity transplantation
| Mouse type . | n . | Pathology . | |||
|---|---|---|---|---|---|
| Kidney . | Liver . | Spleen . | Bone marrow . | ||
| BERK/mock | 7 | Mesangial proliferation (2/7), E-M hematopoiesis (7/7), focal ischemic lesion (2/7), cystic kidney dilation (1/7), congestion* (7/7) | E-M hematopoiesis (7/7), 1+ liver infarction (3/7), 3+ liver infarction (4/7) | Weight 870 ± 71 mg, severe erythroid hyperplasia (6/7), moderate erythroid hyperplasia (1/7), obliteration of lymphoid follicles (7/7) | Severe erythroid hyperplasia (6/7), moderate erythroid hyperplasia (1/7) |
| GbG<10 | 4 | Focal segmental lesion (1/4), focal tubular atrophy (1/4), congestion (4/4) | 1+ liver infarction (2/4), 2+ liver infarction (1/4), hemosiderosis (1/4) | Weight 717 ± 162 mg, severe erythroid hyperplasia (1/4), moderate erythroid hyperplasia (2/4), mild erythroid hyperplasia (1/4), occasional lymphoid follicles (2/4) | Mild erythroid hyperplasia (1/3), moderate erythroid hyperplasia (2/3) |
| GbG≥10 | 4 | Mild congestion (1/4), focal tubular atrophy (1/4), normal kidney (2/4) | Normal liver (4/4) | Weight 363 ± 85 mg, mild erythroid hyperplasia (4/4), multiple lymphoid follicles (4/4), congestion (4/4) | Mild erythroid hyperplasia (2/2) |
| Mouse type . | n . | Pathology . | |||
|---|---|---|---|---|---|
| Kidney . | Liver . | Spleen . | Bone marrow . | ||
| BERK/mock | 7 | Mesangial proliferation (2/7), E-M hematopoiesis (7/7), focal ischemic lesion (2/7), cystic kidney dilation (1/7), congestion* (7/7) | E-M hematopoiesis (7/7), 1+ liver infarction (3/7), 3+ liver infarction (4/7) | Weight 870 ± 71 mg, severe erythroid hyperplasia (6/7), moderate erythroid hyperplasia (1/7), obliteration of lymphoid follicles (7/7) | Severe erythroid hyperplasia (6/7), moderate erythroid hyperplasia (1/7) |
| GbG<10 | 4 | Focal segmental lesion (1/4), focal tubular atrophy (1/4), congestion (4/4) | 1+ liver infarction (2/4), 2+ liver infarction (1/4), hemosiderosis (1/4) | Weight 717 ± 162 mg, severe erythroid hyperplasia (1/4), moderate erythroid hyperplasia (2/4), mild erythroid hyperplasia (1/4), occasional lymphoid follicles (2/4) | Mild erythroid hyperplasia (1/3), moderate erythroid hyperplasia (2/3) |
| GbG≥10 | 4 | Mild congestion (1/4), focal tubular atrophy (1/4), normal kidney (2/4) | Normal liver (4/4) | Weight 363 ± 85 mg, mild erythroid hyperplasia (4/4), multiple lymphoid follicles (4/4), congestion (4/4) | Mild erythroid hyperplasia (2/2) |
E-M indicates extramedullary; and 1+ liver infarction, 1 infarction/section.
Congestion of vessels and presence of sickle RBC in vessels. Notably, congested vessels were visible in spleens only when erythroid hyperplasia effacing splenic architecture was reduced. The terminology used to quantify organ pathology is the same as documented in Table 2.