Correction of organ pathology in GbG mice that underwent transplantation after myeloablative conditioning
| . | Kidney . | Liver . | Spleen . | Bone marrow . |
|---|---|---|---|---|
| Mock mice, n = 5 | Focal tubular atrophy (1/5), mild congestion (3/5) | 2+ liver infarction (1/5), 3+ liver infarction (1/5), E-M hematopoiesis (2/5) | Weight 500 ± 60 mg, severe erythroid hyperplasia (5/5) | Severe erythroid hyperplasia (5/5) |
| GbG mice, n = 5 | Normal kidney (5/5) | Normal liver (5/5) | Weight 256 ± 51 mg, mild erythroid hyperplasia (5/5), mild congestion (5/5) | Mild erythroid hyperplasia (5/5) |
| . | Kidney . | Liver . | Spleen . | Bone marrow . |
|---|---|---|---|---|
| Mock mice, n = 5 | Focal tubular atrophy (1/5), mild congestion (3/5) | 2+ liver infarction (1/5), 3+ liver infarction (1/5), E-M hematopoiesis (2/5) | Weight 500 ± 60 mg, severe erythroid hyperplasia (5/5) | Severe erythroid hyperplasia (5/5) |
| GbG mice, n = 5 | Normal kidney (5/5) | Normal liver (5/5) | Weight 256 ± 51 mg, mild erythroid hyperplasia (5/5), mild congestion (5/5) | Mild erythroid hyperplasia (5/5) |
2+ liver infarction indicates 2 to 3 infarctions/section; 3+ liver infarction, more than 3 infarctions/section; and E-M, extramedullary.
Mild congestion of the spleen vessels with sickle RBCs is seen when splenic architecture is restored. This is not noted when the splenic architecture is effaced by extramedullary erythropoiesis. Splenic erythroid hyperplasia: severe is complete obliteration of splenic follicles; moderate, more than 1 follicle present/section; and mild, preservation of follicles with evidence of erythroid islands.
Bone marrow: normal erythropoiesis indicates M/E = 5:2; mild erythroid hyperplasia, M/E = 2:1; moderate erythroid hyperplasia, M:E = 1:1; and severe erythroid hyperplasia, M/E = 1:3. Bone marrow erythropoiesis expressed as myeloid-erythroid ratio (M/E). Numbers in parentheses indicate the histologic feature seen in the number of mice/total number of mice analyzed in that group.