Mutation characteristics in >STAT6 exons 12-14
| . | No. of mutations observed . | No. of motifs or dinucleotides in sequence . | No. of expected mutations* . | Observed/expected mutations ratio . | P† . |
|---|---|---|---|---|---|
| Transversions/transitions | |||||
| All | 22/6 | 18.7/9.3 | 1.83 | .230 | |
| A:T base pairs | 20 | 158 | 12.1 | 1.65 | .004 |
| G:C base pairs | 8 | 207 | 15.9 | 0.5 | |
| Dinucleotides | |||||
| AA‡ | 13 | 20 | 3.077 | 4.225 | < .001 |
| AC | 4 | 19 | 2.923 | 1.368 | .520 |
| AG‡ | 7 | 20 | 3.077 | 2.275 | .025 |
| AT‡ | 7 | 13 | 2.000 | 3.500 | .001 |
| CA | 8 | 27 | 4.154 | 1.926 | .056 |
| CC | 0 | 34 | 5.231 | 0.000 | .007 |
| CG | 0 | 9 | 1.385 | 0.000 | .372 |
| CT | 1 | 32 | 4.923 | 0.203 | .051 |
| GA‡ | 10 | 23 | 3.538 | 2.826 | .001 |
| GC | 1 | 24 | 3.692 | 0.271 | .161 |
| GG | 0 | 33 | 5.077 | 0.000 | .007 |
| GT | 0 | 24 | 3.692 | 0.000 | .041 |
| TA | 0 | 2 | 0.308 | 0.000 | > .999 |
| TC | 0 | 26 | 4.000 | 0.000 | .025 |
| TG | 5 | 42 | 6.462 | 0.774 | .671 |
| TT | 0 | 16 | 2.462 | 0.000 | .155 |
| . | No. of mutations observed . | No. of motifs or dinucleotides in sequence . | No. of expected mutations* . | Observed/expected mutations ratio . | P† . |
|---|---|---|---|---|---|
| Transversions/transitions | |||||
| All | 22/6 | 18.7/9.3 | 1.83 | .230 | |
| A:T base pairs | 20 | 158 | 12.1 | 1.65 | .004 |
| G:C base pairs | 8 | 207 | 15.9 | 0.5 | |
| Dinucleotides | |||||
| AA‡ | 13 | 20 | 3.077 | 4.225 | < .001 |
| AC | 4 | 19 | 2.923 | 1.368 | .520 |
| AG‡ | 7 | 20 | 3.077 | 2.275 | .025 |
| AT‡ | 7 | 13 | 2.000 | 3.500 | .001 |
| CA | 8 | 27 | 4.154 | 1.926 | .056 |
| CC | 0 | 34 | 5.231 | 0.000 | .007 |
| CG | 0 | 9 | 1.385 | 0.000 | .372 |
| CT | 1 | 32 | 4.923 | 0.203 | .051 |
| GA‡ | 10 | 23 | 3.538 | 2.826 | .001 |
| GC | 1 | 24 | 3.692 | 0.271 | .161 |
| GG | 0 | 33 | 5.077 | 0.000 | .007 |
| GT | 0 | 24 | 3.692 | 0.000 | .041 |
| TA | 0 | 2 | 0.308 | 0.000 | > .999 |
| TC | 0 | 26 | 4.000 | 0.000 | .025 |
| TG | 5 | 42 | 6.462 | 0.774 | .671 |
| TT | 0 | 16 | 2.462 | 0.000 | .155 |
Expected mutation frequencies were calculated according to the sequence composition, assuming random targeting of the mutations at a 28/365 rate for single nucleotide changes and 56/364 rate for dinucleotide mutations.
Statistical comparisons were done with the exact binomial test, which compares the observed frequency to the expected one under null hypothesis.
Dinucleotides significantly targeted by mutations.