Rates of VWF secretion from stimulated and unstimulated HUVECs
| Time, min . | VWF rate, ng/mL/min, average (range) . | |
|---|---|---|
| Stimulated . | Unstimulated . | |
| 5-15 | 9.5 (4.8-14.3) | 1.65 (1.3-2.0) |
| 15-3 | 9.9 (6.0-13.8) | 1.85 (1.8-1.9) |
| 30-60 | 10.9 (1.9-20.0) | 0.69 (0.01-1.4) |
| 60-180 | 1.29 (1.0-1.6) | 0.49 (0-1.4) |
| Time, min . | VWF rate, ng/mL/min, average (range) . | |
|---|---|---|
| Stimulated . | Unstimulated . | |
| 5-15 | 9.5 (4.8-14.3) | 1.65 (1.3-2.0) |
| 15-3 | 9.9 (6.0-13.8) | 1.85 (1.8-1.9) |
| 30-60 | 10.9 (1.9-20.0) | 0.69 (0.01-1.4) |
| 60-180 | 1.29 (1.0-1.6) | 0.49 (0-1.4) |
Cell supernatants were collected from unstimulated and histamine-stimulated HUVECs after 0, 5, 15, 30, 60, and 180 minutes after addition of serum-free media. Rates of VWF secretion (ng/mL per minute) were calculated from averaged VWF levels (average) and, using the lowest and highest VWF levels (range), measured at each time interval in 5 to 6 determinations.