Oligonucleotides and primers used in experiments
| Purpose/primer name . | Primer sequence, 5′ to 3′* . |
|---|---|
| CMV minigenes | |
| M1A-S | ataagcttTGGCAGGAACCTCTTAAAGGGCGAGA |
| M1A 200-As | ctcgagggtaccCTCGACTTTGTGACTCCAGAACCCCT |
| M1A 300-As | ctcgagggtaccCGCTGAGTCCTGCTGCGGACGAACAC |
| M1A 400-As | ctcgagggtaccGATTCAGTTACGTAAGGACCCGAGCA |
| M1B-S | ataagcttAGCTGTGCTGTGTCTCACTTCTGTCC |
| M1B 200-As | ctcgagggtaccAGCCGAGGAAGGATTCTATTCCAAGTCGT |
| M1B 300-As | ctcgagggtaccTCCGCGGGCCTGTGTAGAGGAACAAAT |
| M1B 400-As | ctcgagggtaccTCAAAACGTTCACTGGGCACTACAAC |
| M1C-S | ataagcttAGCCGCAGAGGGCCGGAGCCTCGGAC |
| M1C 200-As | ctcgagggtaccTCCCCCGGCTCCTCGGGCCGCTTCCCA |
| M1C 300-As | ctcgagggtaccTGACTGACAGGCCGGCGCGGACGGACGA |
| M1C 400-As | ctcgagggtaccTCGCTCCGCTCCGCAGCGGCACCCAACA |
| M2-As | atgggccctctagaCTCCAGCTGAGTTTCTTGTTGGCCTGA |
| M1 200-S | ggtaccctcgagGCATATCTTTCATGACTTAAGGCACT |
| M1 300-S | ggtaccctcgagGAGCCACCATGTGGTTGCTGGGAATTG |
| M1 400-S | ggtaccctcgagGGTGTATTTCATTACATGGCCTTGGTAGA |
| Promoter constructs | |
| pM1A-S | atgctagcGGACACACTTTTGGGTCCCAGACTTGG |
| pM1B-S | atacgcgtTTGTCTGTGAAGTGGGAACA |
| pM1C-S | atacgcgtGACTTACCTGAGTTAAGGT |
| UV cross-linking oligos | |
| WT-2′UV-S | ccttttaatagGAACATCATGACAACAGAGAAGAGTTTAGTGGCTGAAGCTGA |
| Mut1-2′UV-S | ccttttaatagGAACATCATGACAACTCTTTCGAGTTTAGTGGCTGAAGCTGA |
| Mut2-2′UV-S | ccttttaatagGAACATCATGACAACAGATAAGAGTTTAGTGGCTGAAGCTGA |
| 2UV-S | acaagcttCCTTTTAATAGGAACATCATG |
| 2′UV-As | actctagaTCAGCTTCAGCCACTAAACT |
| SF2/ASF-shRNA constructs | |
| sh-84 | AACCAAGGACATCGAGGACGTG |
| sh-584 | TGGGCCCAGAAGTCCAAGTTAT |
| sh-726 | CAGATCTCGCTCTCGTACATAA |
| 5'miR30-XhoI | CAGAAGGCTCGAGAAGGTATATTGCTGTTGACAGTGAGCG |
| 3′miR30-EcoRI | CTAAAGTAGCCCCTTGAATTCCGAGGCAGTAGGCA |
| 5′ RACE | |
| GeneRace 5′ Primer | CGACTGGAGCACGAGGACACTGA |
| GeneRace 5′ Nested Primer | GGACACTGACATGGACTGAAGGAGTA |
| 5′Race-MA1-As | CTTTAAGAGGTTCCTGCCACTCT |
| 5′Race-MA2-As | TCCTCGCTCTCCGCGGCTCTCTCCCTTT |
| 5′Race-MB1-As | CCAGAAGTAGTCGTTGTCATCA |
| 5′Race-MB2-As | CATCGTCACGGTGGCACATGGAAGCAAA |
| 5′Race-MC1-As | CGGCCGCAGCTTCAGCCCGACGAACAAT |
| 5′Race-MC2-As | CCGACGAACAATGGCAGGAGGCG |
| Exon 2′/2 splicing analysis | |
| HEx2-RT | GAGGAGAATAGTCGTGAAAGTCCTCTG |
| HEx2-As | GGCTTCCTCACCCTCTTCCTTCTG |
| H1A-S | ataagcttTGGCAGGAACCTCTTAAAGGGCGAGA |
| H1B-S | ataagcttGCTGTGCTGTGTGTCTCACTGCTGTCA |
| H1C-S | ataagcttAGCCGCAGAGGGCCCGAGCCTCGGAC |
| MEx2-RT | GAGGAGAGCAGTCGTGACAGGCCTCTG |
| Luc RRV | GGTTCCATCTTCCAGCGGATAGAA |
| RRV2-As | TGGCGTCTTCCATGGTGGCTTTACCA |
| Real-time PCR | |
| HEx1A-S | ACCTCTTAAAGGGCGAGAGC |
| HEx1A-As | TTCCTTCTGTTGGTGCTGTG |
| HEx1C-S | CCTCCTCCTGCCATTGTTC |
| HEx1C-As | GTGAATTTTCGGCCTCAGTC |
| Purpose/primer name . | Primer sequence, 5′ to 3′* . |
|---|---|
| CMV minigenes | |
| M1A-S | ataagcttTGGCAGGAACCTCTTAAAGGGCGAGA |
| M1A 200-As | ctcgagggtaccCTCGACTTTGTGACTCCAGAACCCCT |
| M1A 300-As | ctcgagggtaccCGCTGAGTCCTGCTGCGGACGAACAC |
| M1A 400-As | ctcgagggtaccGATTCAGTTACGTAAGGACCCGAGCA |
| M1B-S | ataagcttAGCTGTGCTGTGTCTCACTTCTGTCC |
| M1B 200-As | ctcgagggtaccAGCCGAGGAAGGATTCTATTCCAAGTCGT |
| M1B 300-As | ctcgagggtaccTCCGCGGGCCTGTGTAGAGGAACAAAT |
| M1B 400-As | ctcgagggtaccTCAAAACGTTCACTGGGCACTACAAC |
| M1C-S | ataagcttAGCCGCAGAGGGCCGGAGCCTCGGAC |
| M1C 200-As | ctcgagggtaccTCCCCCGGCTCCTCGGGCCGCTTCCCA |
| M1C 300-As | ctcgagggtaccTGACTGACAGGCCGGCGCGGACGGACGA |
| M1C 400-As | ctcgagggtaccTCGCTCCGCTCCGCAGCGGCACCCAACA |
| M2-As | atgggccctctagaCTCCAGCTGAGTTTCTTGTTGGCCTGA |
| M1 200-S | ggtaccctcgagGCATATCTTTCATGACTTAAGGCACT |
| M1 300-S | ggtaccctcgagGAGCCACCATGTGGTTGCTGGGAATTG |
| M1 400-S | ggtaccctcgagGGTGTATTTCATTACATGGCCTTGGTAGA |
| Promoter constructs | |
| pM1A-S | atgctagcGGACACACTTTTGGGTCCCAGACTTGG |
| pM1B-S | atacgcgtTTGTCTGTGAAGTGGGAACA |
| pM1C-S | atacgcgtGACTTACCTGAGTTAAGGT |
| UV cross-linking oligos | |
| WT-2′UV-S | ccttttaatagGAACATCATGACAACAGAGAAGAGTTTAGTGGCTGAAGCTGA |
| Mut1-2′UV-S | ccttttaatagGAACATCATGACAACTCTTTCGAGTTTAGTGGCTGAAGCTGA |
| Mut2-2′UV-S | ccttttaatagGAACATCATGACAACAGATAAGAGTTTAGTGGCTGAAGCTGA |
| 2UV-S | acaagcttCCTTTTAATAGGAACATCATG |
| 2′UV-As | actctagaTCAGCTTCAGCCACTAAACT |
| SF2/ASF-shRNA constructs | |
| sh-84 | AACCAAGGACATCGAGGACGTG |
| sh-584 | TGGGCCCAGAAGTCCAAGTTAT |
| sh-726 | CAGATCTCGCTCTCGTACATAA |
| 5'miR30-XhoI | CAGAAGGCTCGAGAAGGTATATTGCTGTTGACAGTGAGCG |
| 3′miR30-EcoRI | CTAAAGTAGCCCCTTGAATTCCGAGGCAGTAGGCA |
| 5′ RACE | |
| GeneRace 5′ Primer | CGACTGGAGCACGAGGACACTGA |
| GeneRace 5′ Nested Primer | GGACACTGACATGGACTGAAGGAGTA |
| 5′Race-MA1-As | CTTTAAGAGGTTCCTGCCACTCT |
| 5′Race-MA2-As | TCCTCGCTCTCCGCGGCTCTCTCCCTTT |
| 5′Race-MB1-As | CCAGAAGTAGTCGTTGTCATCA |
| 5′Race-MB2-As | CATCGTCACGGTGGCACATGGAAGCAAA |
| 5′Race-MC1-As | CGGCCGCAGCTTCAGCCCGACGAACAAT |
| 5′Race-MC2-As | CCGACGAACAATGGCAGGAGGCG |
| Exon 2′/2 splicing analysis | |
| HEx2-RT | GAGGAGAATAGTCGTGAAAGTCCTCTG |
| HEx2-As | GGCTTCCTCACCCTCTTCCTTCTG |
| H1A-S | ataagcttTGGCAGGAACCTCTTAAAGGGCGAGA |
| H1B-S | ataagcttGCTGTGCTGTGTGTCTCACTGCTGTCA |
| H1C-S | ataagcttAGCCGCAGAGGGCCCGAGCCTCGGAC |
| MEx2-RT | GAGGAGAGCAGTCGTGACAGGCCTCTG |
| Luc RRV | GGTTCCATCTTCCAGCGGATAGAA |
| RRV2-As | TGGCGTCTTCCATGGTGGCTTTACCA |
| Real-time PCR | |
| HEx1A-S | ACCTCTTAAAGGGCGAGAGC |
| HEx1A-As | TTCCTTCTGTTGGTGCTGTG |
| HEx1C-S | CCTCCTCCTGCCATTGTTC |
| HEx1C-As | GTGAATTTTCGGCCTCAGTC |
CMV indicates cytomegalovirus; PCR, polymerase chain reaction; 5′ RACE, 5′ rapid amplification of cDNA ends; and UV, ultraviolet.
Lowercase letters in sequences indicate linkers.