Cutoff values for “false BCR-ABL+ cells,” as reported in previous studies, by the use of I-FISH
| References . | Probe used . | Cutoff . | Samples . |
|---|---|---|---|
| Dewald et al, 199819 | D-FISH | Not identified* | 35 normal subjects |
| Buño et al, 199820 | D-FISH | 0.8% | PBL from 10 patients without any malignant hematologic disorder and BM from 10 normal bone marrow transplant donors |
| Chomel et al, 20009 | D-FISH | 1%† | PBL or BM from 5 patients with hematologic disorders other than CML |
| Le Gouill et al, 200022 | ES | 1.2%† | 14 BM from AML or BCR/ABL− myeloproliferative syndrome patients and 10 PBL from healthy donors |
| Chase et al, 200035 | DCDF | 1%‡ | Not specified |
| Primo et al, 200336 | ES | 0.9%§ | 20 BCR/ABL− BM samples |
| Raanani et al, 200426 | ES | 1%‖ | Not specified |
| El-Zimaity et al, 200437 | ES | 1.2%† | 20 normal control samples |
| References . | Probe used . | Cutoff . | Samples . |
|---|---|---|---|
| Dewald et al, 199819 | D-FISH | Not identified* | 35 normal subjects |
| Buño et al, 199820 | D-FISH | 0.8% | PBL from 10 patients without any malignant hematologic disorder and BM from 10 normal bone marrow transplant donors |
| Chomel et al, 20009 | D-FISH | 1%† | PBL or BM from 5 patients with hematologic disorders other than CML |
| Le Gouill et al, 200022 | ES | 1.2%† | 14 BM from AML or BCR/ABL− myeloproliferative syndrome patients and 10 PBL from healthy donors |
| Chase et al, 200035 | DCDF | 1%‡ | Not specified |
| Primo et al, 200336 | ES | 0.9%§ | 20 BCR/ABL− BM samples |
| Raanani et al, 200426 | ES | 1%‖ | Not specified |
| El-Zimaity et al, 200437 | ES | 1.2%† | 20 normal control samples |
Landstrom et al,27 in a study of Ph+ CML, used a cutoff of 0.6%, citing Dewald et al.19
AML indicates acute myeloid leukemia; D-FISH, dual FISH (from Q-Biogene); DCDF, dual-color dual-fusion (from Vysis-Abbott); ES, extra-signal FISH (from Vysis-Abbott); BM, bone marrow; PBL, peripheral blood.
“Based on 200 nuclei, the mean percentage of false-positive cells was 0.25 ± 0.39. The normal cutoff for 6000 nuclei (for each of 3 normal specimens) was 0.079%.”
Mean + 3 SD.
“Although in experienced hands with good quality preparations the technique can give a very low false positive rate of 0.1%-0.2%, in practice we prefer to use cutoff rate of 1% because accuracy is seriously compromised by several factors, including slide quality and operator experience.”
Mean + 2SD.
“Because the FISH (ES) method has false positive rate of 1%, results were considered negative when < 1% of interphases nuclei were positive for BCR-ABL double-labeled probes.”