Differential role of Rac1 and PKC in regulating alpha-granule secretion from platelets
| Treatment . | P-selectin expression (% positive, mean ± SD, n=4) . | |||
|---|---|---|---|---|
| 0 seconds . | 15 seconds . | 180 seconds . | 300 seconds . | |
| Thr (0.5 U/mL) | 1.7 ± 1.2 | 43.6 ± 18.2 | 59.3 ± 12.4 | n.d |
| NSC23766 (300 μM) + Thr (0.5 U/mL) | 1.5 ± 1.2 | 5.2 ± 2.5 | 12.5 ± 5.2 | n.d |
| Gö6983 (2 μM) + Thr (0.5 U/mL) | 1.4 ± 0.8 | 4.0 ± 1.9 | 10.8 ± 1.8 | n.d |
| Thr (0.1 U/mL) | 2.2 ± 1.5 | 6.6 ± 1.3 | 16.0 ± 3.0 | n.d |
| NSC23766 (30 μM) + Thr (0.1 U/mL) | 1.9 ± 0.6 | 2.9 ± 0.8 | 6.6 ± 1.0 | n.d |
| NSC23766 (50 μM) + Thr (0.1 U/mL) | 1.8 ± 0.5 | 2.5 ± 1.1 | 4.4 ± 1.5 | n.d |
| PdBu (200 nM) | 1.9 ± 2.3 | 1.2 ± 0.7 | 12.2 ± 8.0 | 24.5 ± 15.5 |
| NSC23766 (300 μM) + PdBu (200 nM) | 1.3 ± 0.8 | 1.4 ± 0.7 | 9.0 ± 5.4 | 19.2 ± 7.3 |
| Gö6983 (2 μM) + PdBu (200 nM) | 1.4 ± 0.9 | 1.8 ± 0.4 | 1.9 ± 1.1 | 1.6 ± 1.0 |
| Treatment . | P-selectin expression (% positive, mean ± SD, n=4) . | |||
|---|---|---|---|---|
| 0 seconds . | 15 seconds . | 180 seconds . | 300 seconds . | |
| Thr (0.5 U/mL) | 1.7 ± 1.2 | 43.6 ± 18.2 | 59.3 ± 12.4 | n.d |
| NSC23766 (300 μM) + Thr (0.5 U/mL) | 1.5 ± 1.2 | 5.2 ± 2.5 | 12.5 ± 5.2 | n.d |
| Gö6983 (2 μM) + Thr (0.5 U/mL) | 1.4 ± 0.8 | 4.0 ± 1.9 | 10.8 ± 1.8 | n.d |
| Thr (0.1 U/mL) | 2.2 ± 1.5 | 6.6 ± 1.3 | 16.0 ± 3.0 | n.d |
| NSC23766 (30 μM) + Thr (0.1 U/mL) | 1.9 ± 0.6 | 2.9 ± 0.8 | 6.6 ± 1.0 | n.d |
| NSC23766 (50 μM) + Thr (0.1 U/mL) | 1.8 ± 0.5 | 2.5 ± 1.1 | 4.4 ± 1.5 | n.d |
| PdBu (200 nM) | 1.9 ± 2.3 | 1.2 ± 0.7 | 12.2 ± 8.0 | 24.5 ± 15.5 |
| NSC23766 (300 μM) + PdBu (200 nM) | 1.3 ± 0.8 | 1.4 ± 0.7 | 9.0 ± 5.4 | 19.2 ± 7.3 |
| Gö6983 (2 μM) + PdBu (200 nM) | 1.4 ± 0.9 | 1.8 ± 0.4 | 1.9 ± 1.1 | 1.6 ± 1.0 |
Platelets were untreated or pretreated with inhibitors and then stimulated with thrombin or PdBu. P-selectin expression was measured using the flow cytometer after collecting 10 000 platelet events.