Surface expression of CCR7 on alloreactive and nonalloreactive NK-cell clones after coculture with mDCs
| Clone . | CCR7+,% . | % CCR7+ in the presence of anti–HLA I mAb, % . |
|---|---|---|
| Clone 3, alloreactive | 40 | 45 |
| Clone 4, alloreactive | 60 | 61 |
| Clone 5, alloreactive | 55 | 54 |
| Clone 6, alloreactive | 80 | 82 |
| Clone 7, nonalloreactive | 8 | 51 |
| Clone 8, nonalloreactive | 3.6 | 60 |
| Clone 9, nonalloreactive | 2 | 55 |
| Clone 10, nonalloreactive | 4 | 49 |
| Clone . | CCR7+,% . | % CCR7+ in the presence of anti–HLA I mAb, % . |
|---|---|---|
| Clone 3, alloreactive | 40 | 45 |
| Clone 4, alloreactive | 60 | 61 |
| Clone 5, alloreactive | 55 | 54 |
| Clone 6, alloreactive | 80 | 82 |
| Clone 7, nonalloreactive | 8 | 51 |
| Clone 8, nonalloreactive | 3.6 | 60 |
| Clone 9, nonalloreactive | 2 | 55 |
| Clone 10, nonalloreactive | 4 | 49 |
Natural killer (NK)–cell clones expressing either C1-specific or C2-specific killer immunoglobulin-like receptors were analyzed by immunofluorescence and fluorescence-activated cell-sorting analysis for the expression of C-C chemokine receptor type 7 (CCR7) molecule after 1 hour of coculture with mature dendritic cells (mDCs) from donors expressing human leukocyte antigen (HLA)–C alleles belonging to the C1 specificity, in the absence or presence of anti–HLA class I monoclonal antibody.