E12.5 Placenta-derived long-term repopulating cells are CD150−CD48−
| Placenta cell population . | % of population . | Experimental Enrichment* . | Number of embryo equivalents transplanted . | Number of placenta cells transplanted, × 103 . | Number of reconstituted/transplanted . | % reconstitution long-term†‡ . |
|---|---|---|---|---|---|---|
| c-Kit+CD48−§ | 12 | 8 | 0.5 | 31.5 | 1/1 | 86 |
| c-Kit+CD48+§ | 8 | 12 | 0.5 | 22.3 | 0/5 | |
| c-Kit+CD34medCD48−¶ | 1.5-9 | 11-69 | 0.2-0.75 | 3.2-46.5 | 7/8 | 0.75-90 |
| c-Kit+CD34medCD48+¶ | 1.7-4.6 | 21-54 | 0.2-1 | 4.6-14 | 0/18 | |
| CD48−§ | 72 | 1.4 | 0.5 | 115 | 1/1 | 93 |
| CD48+§ | 24 | 4 | 0.75 | 36.8 | 0/2 | |
| CD41−CD48−§ | 58 | 1.7 | 3 | 150 | 3/3 | 73-91 |
| CD41+CD48−§ | 6.7 | 11.5 | 7.5 | 45 | 2/3 | 70-83‖ |
| CD41−CD48+§ | 18 | 5.4 | 2.75 | 47 | 0/4 | |
| CD41+CD48+§ | 1.5 | 66 | 4 | 4.3 | 0/4 | |
| CD48− (OX78)§ | 92 | 1 | 1 | 168 | 3/3 | 71-78** |
| CD48+ (OX78)§ | 3.2 | 31 | 1 | 25 | 0/2 | |
| CD150−†† | 88-92 | 1-1.2 | 0.5-0.9 | 4.4-10 | 8/8 | 40-86 |
| CD150+†† | 7-7.6 | 11.5-13.9 | 0.5-0.7 | 150-870 | 0/4 |
| Placenta cell population . | % of population . | Experimental Enrichment* . | Number of embryo equivalents transplanted . | Number of placenta cells transplanted, × 103 . | Number of reconstituted/transplanted . | % reconstitution long-term†‡ . |
|---|---|---|---|---|---|---|
| c-Kit+CD48−§ | 12 | 8 | 0.5 | 31.5 | 1/1 | 86 |
| c-Kit+CD48+§ | 8 | 12 | 0.5 | 22.3 | 0/5 | |
| c-Kit+CD34medCD48−¶ | 1.5-9 | 11-69 | 0.2-0.75 | 3.2-46.5 | 7/8 | 0.75-90 |
| c-Kit+CD34medCD48+¶ | 1.7-4.6 | 21-54 | 0.2-1 | 4.6-14 | 0/18 | |
| CD48−§ | 72 | 1.4 | 0.5 | 115 | 1/1 | 93 |
| CD48+§ | 24 | 4 | 0.75 | 36.8 | 0/2 | |
| CD41−CD48−§ | 58 | 1.7 | 3 | 150 | 3/3 | 73-91 |
| CD41+CD48−§ | 6.7 | 11.5 | 7.5 | 45 | 2/3 | 70-83‖ |
| CD41−CD48+§ | 18 | 5.4 | 2.75 | 47 | 0/4 | |
| CD41+CD48+§ | 1.5 | 66 | 4 | 4.3 | 0/4 | |
| CD48− (OX78)§ | 92 | 1 | 1 | 168 | 3/3 | 71-78** |
| CD48+ (OX78)§ | 3.2 | 31 | 1 | 25 | 0/2 | |
| CD150−†† | 88-92 | 1-1.2 | 0.5-0.9 | 4.4-10 | 8/8 | 40-86 |
| CD150+†† | 7-7.6 | 11.5-13.9 | 0.5-0.7 | 150-870 | 0/4 |
Experimental enrichment is calculated by dividing the frequency of the population after sort by the frequency of the population before sort.
Reconstitution is defined as the presence of placenta-derived cells in both the myeloid and lymphoid compartments of the PB of transplanted mice.
Reconstitution presented is based on the frequency of placenta-derived cells in the peripheral blood of mice undergoing transplantation at 19-27 weeks after transplantation.
Data of 1 experiment.
Pooling of data from 4 independent experiments.
Because of contamination of CD41− cells in the CD41+CD48− population, cannot rule out possibility that CD41+CD48− engraftment is CD41− derived.
Eight weeks after transplant.
Pooling of data from 2 independent experiments.