E11.5 AGM-derived long-term repopulating cells are CD150−CD48−
| AGM cell population . | % of population . | Experimental enrichment* . | No. of embryo equivalents transplanted . | No. of AGM cells transplanted, × 104 . | No. reconstituted/no. transplanted . | % of long-term reconstitution †‡ . |
|---|---|---|---|---|---|---|
| CD150+CD48+§ | 0.03 | nd | 1 | 0.08 | 0/1 | |
| CD150−CD48+¶ | 0.9-1.6 | 50-109 | 1.5-2 | 0.4-0.8 | 0/14 | |
| CD150+CD48−‖ | 0.1-0.8 | 38-115 | 1-2 | 0.05-0.5 | 0/3 | |
| CD150−CD48−¶ | 97-98 | 1 | 1-2 | 26-41 | 3/8 | 0.8-26 |
| CD150−CD48−CD41−‖ | 95-96 | 1 | 1.5-2 | 39-42 | 2/6 | 21-63 |
| CD150−CD48−CD41+** | 0.7-0.6 | 120-140 | 1-3.3 | 0.16-0.32 | 4/4 | 1.7-51 |
| CD150+CD48−CD41+§ | 0.34 | 272 | 1.1 | 0.040 | 0/2 | |
| CD150−CD48+CD41+§ | 0.1 | 650 | 5.6 | 0.2 | 0/6 | |
| CD48+ (OX78)§ | 1.6 | 60 | 2 | 1 | 0/1 | |
| CD48− (OX78)§ | 97 | 1 | 2 | 63 | 3/3 | 0.8-43†† |
| AGM cell population . | % of population . | Experimental enrichment* . | No. of embryo equivalents transplanted . | No. of AGM cells transplanted, × 104 . | No. reconstituted/no. transplanted . | % of long-term reconstitution †‡ . |
|---|---|---|---|---|---|---|
| CD150+CD48+§ | 0.03 | nd | 1 | 0.08 | 0/1 | |
| CD150−CD48+¶ | 0.9-1.6 | 50-109 | 1.5-2 | 0.4-0.8 | 0/14 | |
| CD150+CD48−‖ | 0.1-0.8 | 38-115 | 1-2 | 0.05-0.5 | 0/3 | |
| CD150−CD48−¶ | 97-98 | 1 | 1-2 | 26-41 | 3/8 | 0.8-26 |
| CD150−CD48−CD41−‖ | 95-96 | 1 | 1.5-2 | 39-42 | 2/6 | 21-63 |
| CD150−CD48−CD41+** | 0.7-0.6 | 120-140 | 1-3.3 | 0.16-0.32 | 4/4 | 1.7-51 |
| CD150+CD48−CD41+§ | 0.34 | 272 | 1.1 | 0.040 | 0/2 | |
| CD150−CD48+CD41+§ | 0.1 | 650 | 5.6 | 0.2 | 0/6 | |
| CD48+ (OX78)§ | 1.6 | 60 | 2 | 1 | 0/1 | |
| CD48− (OX78)§ | 97 | 1 | 2 | 63 | 3/3 | 0.8-43†† |
Experimental enrichment is calculated by dividing the frequency of the population after sort by the frequency of the population before sort.
Reconstitution is defined as the presence of AGM-derived cells in both the myeloid and lymphoid compartments of the peripheral blood of mice undergoing transplantation.
Reconstitution presented is based on the frequency of AGM-derived cells in the peripheral blood of mice undergoing transplantation at 17-24 weeks after transplantation.
Data from 1 experiment.
Pooling of data from 4 independent experiments.
Pooling of data from 2 independent experiments.
Pooling of data from 3 independent experiments.
Eight weeks after transplantation.