List of antibodies used
| Marker . | Clone . | Isotype . | Supplier . | Application . |
|---|---|---|---|---|
| Rat IgG2a/PE isotype control | R35-95 | Rat IgG2a | BD Biosciences | FC |
| CD124 (IL-4Rα chain)/PE | mIL4R-M1 | Rat IgG2a | BD Biosciences | FC |
| STAT6 (M-20) | Polyclonal | Rabbit Ig | Santa Cruz Biotechnology | WB |
| p-STAT6 (Tyr641) | Polyclonal | Rabbit Ig | Santa Cruz Biotechnology | WB |
| Rat IgG2a/pure isotype control | NA/LE | Rat IgG2a | BD Biosciences | FC |
| Mouse IgG2a/pure isotype control | X39 | Mouse IgG2a | BD Biosciences | IP |
| Anti–rat Ig/PE/APC | Polyclonal | Goat Ig | BD Biosciences | FC |
| CD16/CD32/pure Fc-Block | 2.4G2 | Rat IgG2b | BD Biosciences | FC |
| E-cadherin/pure | 36 | Mouse IgG2a | BD Biosciences | WB, IF, IP |
| E-cadherin/pure | ECCD2 | Rat IgG2a | Dr. M. Takeichi (University of Kyoto, Kyoto, Japan) | FC |
| p120 Catenin/pure | 98 | Mouse IgG1 | BD Biosciences | WB, IF |
| β-Catenin/pure | Polyclonal | Rabbit Ig | Sigma-Aldrich | WB, IF |
| α-Catenin/pure | Polyclonal | Rabbit Ig | Sigma-Aldrich | WB, IF |
| β-Actin/pure | AC-15 | Mouse IgG1 | Abcam | WB |
| CD11c/PerCp-Cy5.5 | N418 | Hamster IgG | eBioscience | FC |
| CD115/bio | AFS98 | Rat IgG2a | eBioscience | FC |
| Ly6c/APC | ER-MP20 | Rat IgG2a | Serotec | FC |
| Ly6c/FITC | AL21 | Rat IgM | BD Biosciences | FC |
| F4/80/APC–Alexa Fluor 750 | BM8 | Rat IgG2a | eBioscience | FC |
| CCR3/FITC | 83101 | Rat IgG2a | R&D Systems | FC |
| Ly6G/PE | 1A8 | Rat IgG2a | BD Biosciences | FC |
| IA/IE/PE | M5/114.15.2 | Rat IgG2b | BD Biosciences | FC |
| IA/IE/FITC | M5/114.15.2 | Rat IgG2b | eBioscience | FC |
| Marker . | Clone . | Isotype . | Supplier . | Application . |
|---|---|---|---|---|
| Rat IgG2a/PE isotype control | R35-95 | Rat IgG2a | BD Biosciences | FC |
| CD124 (IL-4Rα chain)/PE | mIL4R-M1 | Rat IgG2a | BD Biosciences | FC |
| STAT6 (M-20) | Polyclonal | Rabbit Ig | Santa Cruz Biotechnology | WB |
| p-STAT6 (Tyr641) | Polyclonal | Rabbit Ig | Santa Cruz Biotechnology | WB |
| Rat IgG2a/pure isotype control | NA/LE | Rat IgG2a | BD Biosciences | FC |
| Mouse IgG2a/pure isotype control | X39 | Mouse IgG2a | BD Biosciences | IP |
| Anti–rat Ig/PE/APC | Polyclonal | Goat Ig | BD Biosciences | FC |
| CD16/CD32/pure Fc-Block | 2.4G2 | Rat IgG2b | BD Biosciences | FC |
| E-cadherin/pure | 36 | Mouse IgG2a | BD Biosciences | WB, IF, IP |
| E-cadherin/pure | ECCD2 | Rat IgG2a | Dr. M. Takeichi (University of Kyoto, Kyoto, Japan) | FC |
| p120 Catenin/pure | 98 | Mouse IgG1 | BD Biosciences | WB, IF |
| β-Catenin/pure | Polyclonal | Rabbit Ig | Sigma-Aldrich | WB, IF |
| α-Catenin/pure | Polyclonal | Rabbit Ig | Sigma-Aldrich | WB, IF |
| β-Actin/pure | AC-15 | Mouse IgG1 | Abcam | WB |
| CD11c/PerCp-Cy5.5 | N418 | Hamster IgG | eBioscience | FC |
| CD115/bio | AFS98 | Rat IgG2a | eBioscience | FC |
| Ly6c/APC | ER-MP20 | Rat IgG2a | Serotec | FC |
| Ly6c/FITC | AL21 | Rat IgM | BD Biosciences | FC |
| F4/80/APC–Alexa Fluor 750 | BM8 | Rat IgG2a | eBioscience | FC |
| CCR3/FITC | 83101 | Rat IgG2a | R&D Systems | FC |
| Ly6G/PE | 1A8 | Rat IgG2a | BD Biosciences | FC |
| IA/IE/PE | M5/114.15.2 | Rat IgG2b | BD Biosciences | FC |
| IA/IE/FITC | M5/114.15.2 | Rat IgG2b | eBioscience | FC |
FC indicates flow cytometric analysis; WB, immunoblotting; IP, immunoprecipitation; and IF, immunofluorescence microscopy.