Table 1

Animal subjects study design

Animal IDRhesus origin, sexSHIV strainDuration of infection, yearsCD4 T cells*CD8 T cells*CD20 cells*SHIV-RNA† copies/mLInjected dose infusion, mCiImaging time pri,‡ hours
RH598 Indian, male 89.6P 6.5 2314 2820 129 < 110 1.55 48 
CH3397 Chinese, female Uninfected NA 1700 2641 868 NA 1.94 48 
RH4000 Indian, male Uninfected NA 1672 1730 439 NA 1.69 65, 136 
RH600 Indian, male 89.6P 6.5 1408 2295 990 < 110 1.34 48 
RH637 Indian, male 89.6P 6.5 625 1719 187 < 110 1.90 48 
RH638 Indian, male 89.6P 6.5 604 2000 124 < 110 2.04 48 
RH651 Indian, male 89.6P 6.5 423 745 221 < 110 1.37 48 
RH4019 Indian, male DH12R 0.4 194 979 477 < 110 1.60 48 
RH4001 Indian, male DH12R 0.4 71 2460 540 8310 1.67 48 
RHCL6G Indian, male DH12R 1 50 1213 203 7620 1.45 1, 45, 136 
RH4021 Indian, male DH12R 0.4 5 811 313 25093 1.38 48 
Animal IDRhesus origin, sexSHIV strainDuration of infection, yearsCD4 T cells*CD8 T cells*CD20 cells*SHIV-RNA† copies/mLInjected dose infusion, mCiImaging time pri,‡ hours
RH598 Indian, male 89.6P 6.5 2314 2820 129 < 110 1.55 48 
CH3397 Chinese, female Uninfected NA 1700 2641 868 NA 1.94 48 
RH4000 Indian, male Uninfected NA 1672 1730 439 NA 1.69 65, 136 
RH600 Indian, male 89.6P 6.5 1408 2295 990 < 110 1.34 48 
RH637 Indian, male 89.6P 6.5 625 1719 187 < 110 1.90 48 
RH638 Indian, male 89.6P 6.5 604 2000 124 < 110 2.04 48 
RH651 Indian, male 89.6P 6.5 423 745 221 < 110 1.37 48 
RH4019 Indian, male DH12R 0.4 194 979 477 < 110 1.60 48 
RH4001 Indian, male DH12R 0.4 71 2460 540 8310 1.67 48 
RHCL6G Indian, male DH12R 1 50 1213 203 7620 1.45 1, 45, 136 
RH4021 Indian, male DH12R 0.4 5 811 313 25093 1.38 48 

A total of 11 adult monkeys were imaged (median age, 7 years, range, 7-11 years; median weight, 11.3 kg, range, 8.6-15 kg). One uninfected female Chinese rhesus macaque (CH3397), 1 uninfected male Indian rhesus macaque (RH4000), 5 male SHIV89.6P-infected Indian rhesus macaques (RH598, RH600, RH637, RH638, and RH651), and 4 SHIVDH12R-infected (RH4019, RH4001, RHCL6G, and RH4021) Indian rhesus macaques were recruited in this study from 2 completed studies at the National Institutes of Health (NIH). The 5 SHIV89.6P-infected rhesus macaques were selected for the CD4 T-cell counts from a group of rhesus macaques that had been immunized with 2 × 108 infectious units of recombinant modified vaccinia virus Ankara MVA/KB9 as part of another study12  and challenged intrarectally with 20 infectious units of SHIV89.6P. These macaques showed complete control of viral load and high levels of CD4 cell counts after the phase of acute infection after intrarectal viral inoculation. The 4 SHIVDH12R-infected rhesus macaques were challenged with 1.85 × 106 TCID50 SHIVDH12R clone-7. Three of these 4 animals were treated with an experimental antiretroviral drug during the first 2 weeks after the viral inoculation, as part of another study (T. Igarashi, Y.N., M.M., unpublished observations, February, 2007), which led to partial control of viral replication during the chronic phase of the infection. To identify the optimal timing of imaging, 2 animals (RH4000 and RHCL6G), with high and low CD4 T-cell counts, respectively, were imaged at 1, 45, and 136 hours postradiotracer injection (pri) with SPECT encompassing 3 different fields of view (skull, abdomen, and pelvis). Based on these images, the optimal imaging time for the visualization of lymphoid organs was defined at approximately 48 hours pri for the other animals (data not shown), and all subsequent imaging was done at this time.

NA indicates not applicable.

*

Cells/μ L at the time of imaging.

†

SHIV viremia at the time of imaging.

‡

Imaging time postradiotracer injection (pri)

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