Procoagulant and anticoagulant properties of fibroblasts, SMCs, HUVECs, and TNFα-stimulated HUVECs
| . | Fibroblasts . | SMCs . | HUVECs . | |
|---|---|---|---|---|
| Unstimulated . | + TNFα . | |||
| Prothrombinase activity, nM/min | 8.2 ± 2.0 | 8.8 ± 3.4 | 8.3 ± 1.6 | 8.6 ± 1.9 |
| Activated protein C, pM/min | 18.6 ± 13.5* | 45.3 ± 35.1 | 55.0 ± 29.2 | 57.9 ± 12.9 |
| TF activity, pM/min | ||||
| − Anti-TF | 475.0 ± 238.0* | 31.3 ± 13.1* | 3.6 ± 3.8 | 71.6 ± 34.0* |
| + Anti-TF | 109.0 ± 84.8* | 11.0 ± 7.3* | 3.6 ± 3.7 | 11.0 ± 7.3* |
| . | Fibroblasts . | SMCs . | HUVECs . | |
|---|---|---|---|---|
| Unstimulated . | + TNFα . | |||
| Prothrombinase activity, nM/min | 8.2 ± 2.0 | 8.8 ± 3.4 | 8.3 ± 1.6 | 8.6 ± 1.9 |
| Activated protein C, pM/min | 18.6 ± 13.5* | 45.3 ± 35.1 | 55.0 ± 29.2 | 57.9 ± 12.9 |
| TF activity, pM/min | ||||
| − Anti-TF | 475.0 ± 238.0* | 31.3 ± 13.1* | 3.6 ± 3.8 | 71.6 ± 34.0* |
| + Anti-TF | 109.0 ± 84.8* | 11.0 ± 7.3* | 3.6 ± 3.7 | 11.0 ± 7.3* |
SMC indicates smooth muscle cell; HUVEC, human umbilical vein endothelial cell; TNFα, tumor necrosis factor-α; and TF, tissue factor.
P value less than .05 versus HUVECs.