Fibrin network density in clots formed by in situ thrombin generation on fibroblasts, SMCs, HUVECs, and TNFα-stimulated HUVECs
| . | Fibrin network density . | P, 0-10 μm versus 40-50 μm . | |
|---|---|---|---|
| 0-10 μm . | 40-50 μm . | ||
| Fibroblasts | |||
| + Control peptide | 0.796 ± 0.035 | 0.515 ± 0.045 | .001 |
| + GRGDSP | 0.835 ± 0.012 | 0.527 ± 0.054 | .016 |
| P, ± GRGDSP | NS | NS | |
| SMCs | |||
| + Control peptide | 0.683 ± 0.034 | 0.455 ± 0.062 | .001 |
| + GRGDSP | 0.666 ± 0.058 | 0.446 ± 0.001 | .003 |
| P, ± GRGDSP | NS | NS | |
| HUVECs | |||
| + Control peptide | 0.546 ± 0.053 | 0.306 ± 0.050 | .001 |
| + GRGDSP | 0.579 ± 0.114 | 0.364 ± 0.020 | .03 |
| P, ± GRGDSP | NS | NS | |
| HUVECs + TNFα | |||
| + Control peptide | 0.723 ± 0.037 | 0.468 ± 0.011 | .001 |
| + GRGDSP | 0.730 ± 0.005 | 0.475 ± 0.059 | .026 |
| P, ± GRGDSP | NS | NS | |
| . | Fibrin network density . | P, 0-10 μm versus 40-50 μm . | |
|---|---|---|---|
| 0-10 μm . | 40-50 μm . | ||
| Fibroblasts | |||
| + Control peptide | 0.796 ± 0.035 | 0.515 ± 0.045 | .001 |
| + GRGDSP | 0.835 ± 0.012 | 0.527 ± 0.054 | .016 |
| P, ± GRGDSP | NS | NS | |
| SMCs | |||
| + Control peptide | 0.683 ± 0.034 | 0.455 ± 0.062 | .001 |
| + GRGDSP | 0.666 ± 0.058 | 0.446 ± 0.001 | .003 |
| P, ± GRGDSP | NS | NS | |
| HUVECs | |||
| + Control peptide | 0.546 ± 0.053 | 0.306 ± 0.050 | .001 |
| + GRGDSP | 0.579 ± 0.114 | 0.364 ± 0.020 | .03 |
| P, ± GRGDSP | NS | NS | |
| HUVECs + TNFα | |||
| + Control peptide | 0.723 ± 0.037 | 0.468 ± 0.011 | .001 |
| + GRGDSP | 0.730 ± 0.005 | 0.475 ± 0.059 | .026 |
| P, ± GRGDSP | NS | NS | |
SMC indicates smooth muscle cell; HUVEC, human umbilical vein endothelial cell; TNFα, tumor necrosis factor-α; and NS, not significantly different with control (GRDESP) or β3-blocking (GRDGSP) peptide.