Thrombin generation and fibrin formation supported by fibroblasts, SMCs, HUVECs, and TNFα-stimulated HUVECs
| . | Fibroblasts . | SMCs . | HUVECs . | |
|---|---|---|---|---|
| Unstimulated . | + TNFα . | |||
| Thrombin generation | ||||
| Lag time, min | 1.7 ± 0.2* | 3.6 ± 0.6* | 12.8 ± 1.8 | 2.4 ± 0.2* |
| Rate, nM/min | 60.5 ± 4.0* | 14.9 ± 1.9* | 1.5 ± 0.4 | 14.3 ± 4.0* |
| Time to peak, min | 4.8 ± 0.3* | 10.1 ± 1.0* | 27.6 ± 3.0 | 9.0 ± 0.8* |
| Peak height, nM | 189.1 ± 8.1* | 95.6 ± 6.6* | 22.4 ± 6.4 | 91.4 ± 13.8* |
| Clot formation | ||||
| Clotting onset, min | 1.9 ± 0.6* | 2.5 ± 0.4* | 22.6 ± 7.7 | 2.1 ± 0.7* |
| Fibrin polymerization rate, mOD/min | 238.5 ± 58.3* | 138.4 ± 18.5* | 56.5 ± 28.9 | 109.5 ± 34.4* |
| Final turbidity at 405 nm | 0.822 ± 0.030 | 0.842 ± 0.020 | 0.787 ± 0.040 | 0.830 ± 0.060 |
| . | Fibroblasts . | SMCs . | HUVECs . | |
|---|---|---|---|---|
| Unstimulated . | + TNFα . | |||
| Thrombin generation | ||||
| Lag time, min | 1.7 ± 0.2* | 3.6 ± 0.6* | 12.8 ± 1.8 | 2.4 ± 0.2* |
| Rate, nM/min | 60.5 ± 4.0* | 14.9 ± 1.9* | 1.5 ± 0.4 | 14.3 ± 4.0* |
| Time to peak, min | 4.8 ± 0.3* | 10.1 ± 1.0* | 27.6 ± 3.0 | 9.0 ± 0.8* |
| Peak height, nM | 189.1 ± 8.1* | 95.6 ± 6.6* | 22.4 ± 6.4 | 91.4 ± 13.8* |
| Clot formation | ||||
| Clotting onset, min | 1.9 ± 0.6* | 2.5 ± 0.4* | 22.6 ± 7.7 | 2.1 ± 0.7* |
| Fibrin polymerization rate, mOD/min | 238.5 ± 58.3* | 138.4 ± 18.5* | 56.5 ± 28.9 | 109.5 ± 34.4* |
| Final turbidity at 405 nm | 0.822 ± 0.030 | 0.842 ± 0.020 | 0.787 ± 0.040 | 0.830 ± 0.060 |
SMC indicates smooth muscle cell; HUVEC, human umbilical vein endothelial cell; TNFα, tumor necrosis factor-α; and mOD, milli-optical density unit.
P value less than .05 versus unstimulated HUVECs.