Table 1

Primer sets used for RT-PCR characterization

CytokineAccesson no.DefinitionForward primerReverse primerProduct size, bp
zkitla AY929068 Danio rerio kit ligand a* GGATTCAATGCTTGACTTTG TGTACTATGTTGCGCTGATG 205 
zkitlb AY929069 Danio rerio kit ligand b* CTTGATTGCCGTTCAGTTAG TGACCGGAGATTGTAGACAC 107 
zbmp 1 DQ424857 Danio rerio bone morphogenic protein 1* GGATGGATATTGGAGGAAAG CTTTGTTCGGTCTGTAATCG 230 
zil-1 la BN000717 Danio rerio interleukin-l la* GACAAGCTGAGCAATCAGAC GGAGCTGAGAAAGAGTAGGC 172 
zil-1lb BN000718 Danio rerio interleukin-11b* TTGAACATTCGCTATCATCC GAGTAATCGTTCCCCAATTC 166 
zdla NM 130954 Danio rerio δA† ACGACGATTTGAGTATGACG GGGATTGGCACTTTATATCC 186 
zdlb NM 130958 Danio rerio δB† TTCCGTGTTTAATGATTTGG CACTCCACAGAAACTCTTGC 158 
zdlc NM 130944 Danio rerio δC† TGGTGGACTACAATCTGAGC ACCTCAGTAGCAAACACACG 169 
zdld NM 130955 Danio rerio δD† TGAAACATTACCAAGCCAAC GCAGTGCTTCAATAATCAGC 189 
zdll4 NM 001079835 Danio rerio δ-like 4† CTCTTTCAGCACACCAATTC TGAACATCCTGAGACCATTC 189 
zjagla BC162468 Danio rerio jagged 1a† TGATTGGTGGATACTTCTGC AATCCATTGAGTGTGTCCTG 238 
zjaglb NM 131863 Danio rerio jagged 1b† CTGTGAGCCATCTTCTTCAG AGCAAAGGAACCAGGTAGTC 213 
zjag2 BC163889 Danio rerio jagged 2† AATGACTGTGTGAGCAATCC GTCATTGACCAGATCCACAC 174 
zil-12a NM 001007107 Danio rerio interleukin 12a‡ GTGAGTCTGCTGAAGGAGTG AGTGACATCATTTCCTGTGC 167 
zil-15–2 AB194243 Danio rerio interleukin 15–2‡ CCAAGTCCACAATTACATGC TCTTTGTAGAGCTCGCAGAC 166 
zil-10 AY887900 Danio rerio interleukin 10‡ ATGAATCCAACGATGACTTG TCTTGCATTTCACCATATCC 222 
zil-22 NM 001020792 Danio rerio interleukin 22‡ CTACCTGCGATATGAAGTGC GAAATATGGAAGCAGTCGTG 173 
zil-26 NM 001020799 Danio rerio interleukin 26 TGAAAAGATGTGGGATGAAC ACTGATCCACAGCAAAACAC 214 
zepo NM001 115128 Danio rerio erythropoietin transcript variant 1‡ ACTTGTAAGGACGATTGCAG TATCTGTAATGAGCCGATGG 156 
zfgfl1 N M200760 Danio rerio fibroblast growth factor 1‡ ATACTGCGCATAAAAGCAAC AGTGGTTTTCCTCCATCTTC 154 
zfgf2l N M001045324 Danio rerio fibroblast growth factor 21‡ CGGTGGTGTATGTATGTTCC GTAGCTGCACTCTGGATGAC 203 
CytokineAccesson no.DefinitionForward primerReverse primerProduct size, bp
zkitla AY929068 Danio rerio kit ligand a* GGATTCAATGCTTGACTTTG TGTACTATGTTGCGCTGATG 205 
zkitlb AY929069 Danio rerio kit ligand b* CTTGATTGCCGTTCAGTTAG TGACCGGAGATTGTAGACAC 107 
zbmp 1 DQ424857 Danio rerio bone morphogenic protein 1* GGATGGATATTGGAGGAAAG CTTTGTTCGGTCTGTAATCG 230 
zil-1 la BN000717 Danio rerio interleukin-l la* GACAAGCTGAGCAATCAGAC GGAGCTGAGAAAGAGTAGGC 172 
zil-1lb BN000718 Danio rerio interleukin-11b* TTGAACATTCGCTATCATCC GAGTAATCGTTCCCCAATTC 166 
zdla NM 130954 Danio rerio δA† ACGACGATTTGAGTATGACG GGGATTGGCACTTTATATCC 186 
zdlb NM 130958 Danio rerio δB† TTCCGTGTTTAATGATTTGG CACTCCACAGAAACTCTTGC 158 
zdlc NM 130944 Danio rerio δC† TGGTGGACTACAATCTGAGC ACCTCAGTAGCAAACACACG 169 
zdld NM 130955 Danio rerio δD† TGAAACATTACCAAGCCAAC GCAGTGCTTCAATAATCAGC 189 
zdll4 NM 001079835 Danio rerio δ-like 4† CTCTTTCAGCACACCAATTC TGAACATCCTGAGACCATTC 189 
zjagla BC162468 Danio rerio jagged 1a† TGATTGGTGGATACTTCTGC AATCCATTGAGTGTGTCCTG 238 
zjaglb NM 131863 Danio rerio jagged 1b† CTGTGAGCCATCTTCTTCAG AGCAAAGGAACCAGGTAGTC 213 
zjag2 BC163889 Danio rerio jagged 2† AATGACTGTGTGAGCAATCC GTCATTGACCAGATCCACAC 174 
zil-12a NM 001007107 Danio rerio interleukin 12a‡ GTGAGTCTGCTGAAGGAGTG AGTGACATCATTTCCTGTGC 167 
zil-15–2 AB194243 Danio rerio interleukin 15–2‡ CCAAGTCCACAATTACATGC TCTTTGTAGAGCTCGCAGAC 166 
zil-10 AY887900 Danio rerio interleukin 10‡ ATGAATCCAACGATGACTTG TCTTGCATTTCACCATATCC 222 
zil-22 NM 001020792 Danio rerio interleukin 22‡ CTACCTGCGATATGAAGTGC GAAATATGGAAGCAGTCGTG 173 
zil-26 NM 001020799 Danio rerio interleukin 26 TGAAAAGATGTGGGATGAAC ACTGATCCACAGCAAAACAC 214 
zepo NM001 115128 Danio rerio erythropoietin transcript variant 1‡ ACTTGTAAGGACGATTGCAG TATCTGTAATGAGCCGATGG 156 
zfgfl1 N M200760 Danio rerio fibroblast growth factor 1‡ ATACTGCGCATAAAAGCAAC AGTGGTTTTCCTCCATCTTC 154 
zfgf2l N M001045324 Danio rerio fibroblast growth factor 21‡ CGGTGGTGTATGTATGTTCC GTAGCTGCACTCTGGATGAC 203 

Primer sets used for RT-PCR characterization of ZKS cells. Gene names are listed at left, followed by accession number and definition listed in the database. Forward and reverse primer sequences are listed 5′ to 3′, and product size is listed at far right. Since not all products span exons, negative RT controls were performed alongside all reactions.

*

Transcripts involved in proliferation and differentiation of hematopoietic stem and progenitor cells.

†

Transcripts involved in signaling, lymphoid development, and differentiation.

‡

Transcripts involved in lineage-specific signaling, maintenance, and differentiation.

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal