Effects of endorepellin on tyrosine phosphorylation of various tyrosine kinase receptors in human endothelial cells
| Receptor . | Endorepellin-evoked Tyr phosphorylation over controls* . | P . | Anti-α2β1 + endorepellin† . | P . | Reported function . |
|---|---|---|---|---|---|
| EGFR | 0.328 ± 0.017 | < .001 | 1.10 ± 0.23 | .505 | Migration, proliferation |
| EphA2 | 0.144 ± 0.056 | < .001 | 0.95 ± 0.08 | .899 | Guidance of cell migration |
| MSP-R | 0.422 ± 0.16 | .035 | 1.16 ± 0.04 | .885 | Migration, proliferation, tubular morphogenesis |
| c-Ret | 0.270 ± 0.096 | .017 | 0.99 ± 0.16 | .829 | Survival, proliferation |
| ROR2 | 0.207 ± 0.073 | .008 | 1.51 ± 0.34 | .232 | Growth |
| Tie2 | 0.212 ± 0.063 | .001 | 1.13 ± 0.15 | .712 | Promotion of angiogenesis |
| VEGFR1 | 0.521 ± 0.029 | < .001 | 1.19 ± 0.20 | .606 | Migration, proliferation, tubular morphogenesis |
| VEGFR2 | 0.441 ± 0.036 | < .001 | 1.08 ± 0.18 | .932 | Migration, proliferation, tubular morphogenesis |
| Receptor . | Endorepellin-evoked Tyr phosphorylation over controls* . | P . | Anti-α2β1 + endorepellin† . | P . | Reported function . |
|---|---|---|---|---|---|
| EGFR | 0.328 ± 0.017 | < .001 | 1.10 ± 0.23 | .505 | Migration, proliferation |
| EphA2 | 0.144 ± 0.056 | < .001 | 0.95 ± 0.08 | .899 | Guidance of cell migration |
| MSP-R | 0.422 ± 0.16 | .035 | 1.16 ± 0.04 | .885 | Migration, proliferation, tubular morphogenesis |
| c-Ret | 0.270 ± 0.096 | .017 | 0.99 ± 0.16 | .829 | Survival, proliferation |
| ROR2 | 0.207 ± 0.073 | .008 | 1.51 ± 0.34 | .232 | Growth |
| Tie2 | 0.212 ± 0.063 | .001 | 1.13 ± 0.15 | .712 | Promotion of angiogenesis |
| VEGFR1 | 0.521 ± 0.029 | < .001 | 1.19 ± 0.20 | .606 | Migration, proliferation, tubular morphogenesis |
| VEGFR2 | 0.441 ± 0.036 | < .001 | 1.08 ± 0.18 | .932 | Migration, proliferation, tubular morphogenesis |
The values represent the relative levels of the phosphorylation status of individual tyrosine kinase receptors (mean ± SEM) evoked by a 5-minute treatment with endorepellin compared with vehicle-treated HUVECs (n ≥ 4). P values were calculated with the Student t test.
The values represent the phosphorylation status of individual tyrosine kinase receptors (mean ± SEM) after coincubation with a function-blocking antibody (mAb 1998z directed toward the α2 subunit of the α2β1 integrin) and endorepellin compared with vehicle-treated HUVECs (n ≥ 4). P values were calculated with the Student t test.