Competitive biodistribution of α4+/+ and α4−/− cells
| . | Total sections . | Cells counted . | Zone 1, % . | Zone 2, % . | Zone 3, % . |
|---|---|---|---|---|---|
| Irradiated recipients | |||||
| 11.5 × 106 green α4+/+ cells + 11.8 × 106 red α4−/− cells | |||||
| CB | 100 | 906 green α4+/+ cells277 red α4−/− cells | 15.55 ± 2.78 10.12 ± 1.12 | 49.03 ± 5.71 46.42 ± 6.2 | 35.55 ± 8.07 43.47 ± 6.14 |
| Tr B | 118 | 4509 green α4+/+ cells 910 red α4+/+ cells | 21.29 ± 3.19 12.53 ± 2.2 | 78.71 ± 3.19 87.47 ± 2.2 | |
| 8.5 × 106 red α4+/+ cells + 11.5 × 106 green α4−/− cells | |||||
| CB | 102 | 556 red α4+/+ cells 676 green α4−/− cells | 13.44 ± 4.011 12.78 ± 2.78 | 43.2 ± 7.89 37.14 ± 5.57 | 43.36 ± 11.81 50.08 ± 8.35 |
| Tr B | 99 | 1839 red α4+/+ cells 1942 green α4−/− cells | 19.1 ± 4.06 16.58 ± 1.68 | 80.9 ± 4.06 85.43 ± 2.47 | |
| Nonirradiated recipients | |||||
| 4.25 × 106 green α4+/+ cells + 8.5 × 106 red α4−/− cells | |||||
| CB | 140 | 26 green α4+/+ cells 1212 red α4−/− cells | 14.0 ± 9.0 8.7 ± 1.5 | 38.5 ± 10.0 29.3 ± 1.6 | 47.5 ± 9.0 61.9 ± 3.0 |
| Tr B | 120 | 72 green α4+/+ cells 2935 red α4−/− cells | 12.0 ± 5.5 11.2 ± 1.2 | 87.9 ± 5.5 88.7 ± 1.2 | |
| . | Total sections . | Cells counted . | Zone 1, % . | Zone 2, % . | Zone 3, % . |
|---|---|---|---|---|---|
| Irradiated recipients | |||||
| 11.5 × 106 green α4+/+ cells + 11.8 × 106 red α4−/− cells | |||||
| CB | 100 | 906 green α4+/+ cells277 red α4−/− cells | 15.55 ± 2.78 10.12 ± 1.12 | 49.03 ± 5.71 46.42 ± 6.2 | 35.55 ± 8.07 43.47 ± 6.14 |
| Tr B | 118 | 4509 green α4+/+ cells 910 red α4+/+ cells | 21.29 ± 3.19 12.53 ± 2.2 | 78.71 ± 3.19 87.47 ± 2.2 | |
| 8.5 × 106 red α4+/+ cells + 11.5 × 106 green α4−/− cells | |||||
| CB | 102 | 556 red α4+/+ cells 676 green α4−/− cells | 13.44 ± 4.011 12.78 ± 2.78 | 43.2 ± 7.89 37.14 ± 5.57 | 43.36 ± 11.81 50.08 ± 8.35 |
| Tr B | 99 | 1839 red α4+/+ cells 1942 green α4−/− cells | 19.1 ± 4.06 16.58 ± 1.68 | 80.9 ± 4.06 85.43 ± 2.47 | |
| Nonirradiated recipients | |||||
| 4.25 × 106 green α4+/+ cells + 8.5 × 106 red α4−/− cells | |||||
| CB | 140 | 26 green α4+/+ cells 1212 red α4−/− cells | 14.0 ± 9.0 8.7 ± 1.5 | 38.5 ± 10.0 29.3 ± 1.6 | 47.5 ± 9.0 61.9 ± 3.0 |
| Tr B | 120 | 72 green α4+/+ cells 2935 red α4−/− cells | 12.0 ± 5.5 11.2 ± 1.2 | 87.9 ± 5.5 88.7 ± 1.2 | |
Cumulative data from irradiated and nonirradiated recipients of both α4+/+ and α4−/− cells labeled with different fluorochromes. Note that the data from these competitive distribution experiments are in line with data shown in Table 1 regarding differences between α4+/+ and α4−/− cells in both cell recoveries and microanatomic distribution after irradiation in compact bone. (The numbers for α4+/+ cells in “Nonirradiated recipients” are not included in Table 1).
CB indicates compact bone; Tr B, trabecular bone.
Boldface indicates significant differences between α4+/+ and α4−/− cells.