Quantitative microanatomic distribution analysis of wild-type or α4 integrin-deficient Lin−c-kit+ donor cells in nonirradiated or irradiated compact or trabecular bone
| . | No. of experiments . | No. of sections . | No. of cells . | Zone 1, % cells . | Zone 2, % cells . | Zone 3, % cells . |
|---|---|---|---|---|---|---|
| Compact bone (femur diaphyses) | ||||||
| α4+/+ | ||||||
| nonirradiated | 3 | 261 | 12 379 | 10.19 ± 0.96* | 35.60 ± 1.51 | 54.2 ± 0.55† |
| irradiated | 6 | 595 | 7825 | 20.58 ± 2.71* | 38.37 ± 1.88 | 41.04 ± 1.88† |
| α4−/− | ||||||
| nonirradiated | 4 | 489 | 10 042 | 8.69 ± 1.04 | 27.82 ± 1.8‡ | 63.49 ± 2.40‡ |
| irradiated | 5 | 465 | 4305 | 8.98 ± 1.44 | 36.44 ± 2.0‡ | 54.58 ± 2.35‡ |
| Trabecular bone (epiphyses, sternum) | ||||||
| α4+/+ | ||||||
| nonirradiated | 3 | 312 | 61 630 | 17.97 ± 1.85§ | 82.03 ± 1.85 | |
| irradiated | 7 | 638 | 45 869 | 18.54 ± 1.18‖ | 81.46 ± 1.18 | |
| α4−/− | ||||||
| nonirradiated | 3 | 325 | 11 141 | 9.44% ± 1.8§ | 90.56 ± 1.87 | |
| irradiated | 4 | 348 | 8049 | 10.66 ± 2.09‖ | 89.34 ± 2.09 |
| . | No. of experiments . | No. of sections . | No. of cells . | Zone 1, % cells . | Zone 2, % cells . | Zone 3, % cells . |
|---|---|---|---|---|---|---|
| Compact bone (femur diaphyses) | ||||||
| α4+/+ | ||||||
| nonirradiated | 3 | 261 | 12 379 | 10.19 ± 0.96* | 35.60 ± 1.51 | 54.2 ± 0.55† |
| irradiated | 6 | 595 | 7825 | 20.58 ± 2.71* | 38.37 ± 1.88 | 41.04 ± 1.88† |
| α4−/− | ||||||
| nonirradiated | 4 | 489 | 10 042 | 8.69 ± 1.04 | 27.82 ± 1.8‡ | 63.49 ± 2.40‡ |
| irradiated | 5 | 465 | 4305 | 8.98 ± 1.44 | 36.44 ± 2.0‡ | 54.58 ± 2.35‡ |
| Trabecular bone (epiphyses, sternum) | ||||||
| α4+/+ | ||||||
| nonirradiated | 3 | 312 | 61 630 | 17.97 ± 1.85§ | 82.03 ± 1.85 | |
| irradiated | 7 | 638 | 45 869 | 18.54 ± 1.18‖ | 81.46 ± 1.18 | |
| α4−/− | ||||||
| nonirradiated | 3 | 325 | 11 141 | 9.44% ± 1.8§ | 90.56 ± 1.87 | |
| irradiated | 4 | 348 | 8049 | 10.66 ± 2.09‖ | 89.34 ± 2.09 |
Summary of all data from nonirradiated or irradiated recipients of transplanted normal (α4+/+) or α4 integrin-deficient HSC populations: between 60 and 150 sections were analyzed in each experiment. Donor cells given were from 7 to 30 × 106 per recipient. Indicated are, for each of the modalities, the number of experiments, the cumulative number of sections and total cells analyzed, and the relative distribution of homed cells to distinct zones within BM (percentage, mean ± SEM). Statistically significant differences between nonirradiation versus irradiation conditioning in compact bone and between α4+/+ and α4−/− in trabecular bone are indicated by boldface. A relative preference for endosteal seeding in irradiated marrow in compact bone was observed for wild-type HSCs, whereas α4 integrin-deficient cells distributed randomly, as in nonirradiated hosts. Microscopic images illustrating the different microanatomic distribution of transplanted wild-type cells in hosts with/without irradiation are shown in Figure 2G and H.
P = .005;
P = .001;
P = .02;
P = .03;
P = .006.