Analysis of EBV specificity within the healthy donor CTL lines
| Peptide . | Lytic . | EBNA1 . | EBNA3 . | Irrelevant . |
|---|---|---|---|---|
| Donor 1 | ||||
| NT CTLs | 243 ± 47 | 1202 ± 154 | 257 ± 47 | 0 ± 0 |
| irr-siRNA+ CTLs | 242 ± 4 | ND | 109 ± 4 | 1 ± 1.7 |
| siRNA4+ CTLs + FK506 | 319 ± 61 | 1050 ± 77 | 248 ± 61 | 1.7 ± 2.1 |
| Donor 2 | ||||
| NT CTLs | 12 ± 1.5 | ND | 143 ± 23 | 0 ± 0 |
| irr-siRNA+ CTLs | 14 ± 3.1 | ND | 224 ± 16 | 0.3 ± 0.6 |
| siRNA4+ CTLs + FK506 | 5 ± 1.2 | ND | 230 ± 16 | 0.7 ± 0.6 |
| Donor 3 | ||||
| NT CTLs | 111 ± 10 | ND | 0 ± 0 | 1.3 ± 1.5 |
| irr-siRNA+ CTLs | 41 ± 10 | ND | 0 ± 0 | 0.7 ± 0.6 |
| siRNA4+ CTLs + FK506 | 64 ± 15 | ND | 0 ± 0 | 2 ± 1 |
| Donor 4 | ||||
| NT CTLs | ND | ND | 701.3 ± 89.5 | 0 ± 0 |
| irr-siRNA+ CTLs | ND | ND | 356 ± 48.1 | 0 ± 0 |
| siRNA4+ CTLs + FK506 | ND | ND | 611 ± 95 | 0 ± 0 |
| Peptide . | Lytic . | EBNA1 . | EBNA3 . | Irrelevant . |
|---|---|---|---|---|
| Donor 1 | ||||
| NT CTLs | 243 ± 47 | 1202 ± 154 | 257 ± 47 | 0 ± 0 |
| irr-siRNA+ CTLs | 242 ± 4 | ND | 109 ± 4 | 1 ± 1.7 |
| siRNA4+ CTLs + FK506 | 319 ± 61 | 1050 ± 77 | 248 ± 61 | 1.7 ± 2.1 |
| Donor 2 | ||||
| NT CTLs | 12 ± 1.5 | ND | 143 ± 23 | 0 ± 0 |
| irr-siRNA+ CTLs | 14 ± 3.1 | ND | 224 ± 16 | 0.3 ± 0.6 |
| siRNA4+ CTLs + FK506 | 5 ± 1.2 | ND | 230 ± 16 | 0.7 ± 0.6 |
| Donor 3 | ||||
| NT CTLs | 111 ± 10 | ND | 0 ± 0 | 1.3 ± 1.5 |
| irr-siRNA+ CTLs | 41 ± 10 | ND | 0 ± 0 | 0.7 ± 0.6 |
| siRNA4+ CTLs + FK506 | 64 ± 15 | ND | 0 ± 0 | 2 ± 1 |
| Donor 4 | ||||
| NT CTLs | ND | ND | 701.3 ± 89.5 | 0 ± 0 |
| irr-siRNA+ CTLs | ND | ND | 356 ± 48.1 | 0 ± 0 |
| siRNA4+ CTLs + FK506 | ND | ND | 611 ± 95 | 0 ± 0 |
Numbers are mean ± SD of interferonγ (IFNγ) spot-forming cells/105 cytotoxic T lymphocytes (CTLs).
EBV indicates Epstein-Barr virus; NT-CTLs, nontransduced cytotoxic T lymphocytes; siRNA, small interfering RNA; irr-siRNA, irrelevant si-RNA; and ND, not determined.