Binding of ADAMTS13 variants to VWF fragments (plate assay)
| VWF construct . | ADAMTS13 construct . | ||
|---|---|---|---|
| ADAMTS13 . | MDTCS . | del(TSP5-CUB) . | |
| VWFA2 | 32.8 ± 3.6 | ||
| VWFA1A2A3 | 82.7 ± 13.4 | 90.8 ± 3.0 | 83.2 ± 4.2 |
| VWFA1CK | 10.0 ± 1.8 | 129 ± 12 | 103 ± 17 |
| VWFD4CK | 143 ± 21 | nb | > 500 |
| VWFA3CK | 100 ± 27 | ||
| VWFB1CK | > 500 | ||
| VWFD4 | > 500 | ||
| VWFD′D3 | nb | ||
| Full-length VWF | 21.6 ± 4.7 | ||
| VWF construct . | ADAMTS13 construct . | ||
|---|---|---|---|
| ADAMTS13 . | MDTCS . | del(TSP5-CUB) . | |
| VWFA2 | 32.8 ± 3.6 | ||
| VWFA1A2A3 | 82.7 ± 13.4 | 90.8 ± 3.0 | 83.2 ± 4.2 |
| VWFA1CK | 10.0 ± 1.8 | 129 ± 12 | 103 ± 17 |
| VWFD4CK | 143 ± 21 | nb | > 500 |
| VWFA3CK | 100 ± 27 | ||
| VWFB1CK | > 500 | ||
| VWFD4 | > 500 | ||
| VWFD′D3 | nb | ||
| Full-length VWF | 21.6 ± 4.7 | ||
Binding of full-length ADAMTS13 and truncated constructs MDTCS and del(TSP5-CUB) to different VWF fragments was determined by plate binding assay in which the VWF fragments were directly immobilized on a microtiter plate. KD(app) values were calculated and reported in nM. Mean values ± SEM are based on 3 to 13 independent experiments. In the case of low-affinity binding, the KD(app) values could not be calculated precisely (> 500). In the case of no binding, the KD(app) values are not applicable
nb indicates no binding.