Expression of macrophage immune response genes in WT and Hfe-deficient macrophages
| Hfe genotype/stimulation gene name . | Hfe+/+ control . | Hfe−/− control . | Hfe+/+S Tm . | Hfe−/−S Tm . |
|---|---|---|---|---|
| iNOS | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.21 ± 0.09* | 0.19 ± 0.06* |
| phox-p47 | 0.68 ± 0.05 | 0.75 ± 0.08 | 1.16 ± 0.25 | 1.25 ± 0.16 |
| TNF-α | 0.03 ± 0.00 | 0.03 ± 0.00 | 2.83 ± 0.35* | 2.21 ± 0.37* |
| IL-1β | 0.01 ± 0.00 | 0.01 ± 0.00 | 175.18 ± 29.84* | 141.71 ± 45.16* |
| IL-6 | 0.31 ± 0.15 | 0.58 ± 0.03 | 296.38 ± 57.90* | 257.65 ± 61.94* |
| IL-10 | 0.24 ± 0.03 | 0.51 ± 0.03 | 3.44 ± 0.64* | 4.59 ± 0.45* |
| IL-12p35 | 0.01 ± 0.00 | 0.02 ± 0.00 | 142.70 ± 22.48* | 148.43 ± 5.82* |
| IL-12/23p40 | 0.15 ± 0.04 | 0.09 ± 0.02 | 377.64 ± 84.73* | 315.80 ± 27.64* |
| IL-18 | 0.39 ± 0.06 | 0.74 ± 0.07 | 3.70 ± 0.86* | 2.86 ± 0.44* |
| IL-23p19 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.90 ± 0.20* | 1.38 ± 0.05* |
| Mip-1α | 0.01 ± 0.00 | 0.01 ± 0.00 | 1.71 ± 0.27* | 1.13 ± 0.22* |
| Mcp-1 | 0.08 ± 0.02 | 0.20 ± 0.03 | 3.75 ± 0.46* | 4.14 ± 2.04* |
| TGF-β | 0.95 ± 0.22 | 0.71 ± 0.10 | 1.56 ± 0.40 | 1.67 ± 0.49 |
| MHC-II | 2.80 ± 0.34 | 2.16 ± 0.57 | 5.69 ± 0.75* | 4.62 ± 0.15* |
| Lcn2 | 1.9 ± 0.6 | 8.3 ± 1.0† | 4434.1 ± 1695.3* | 12 228.3 ± 1149.6*† |
| Hfe genotype/stimulation gene name . | Hfe+/+ control . | Hfe−/− control . | Hfe+/+S Tm . | Hfe−/−S Tm . |
|---|---|---|---|---|
| iNOS | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.21 ± 0.09* | 0.19 ± 0.06* |
| phox-p47 | 0.68 ± 0.05 | 0.75 ± 0.08 | 1.16 ± 0.25 | 1.25 ± 0.16 |
| TNF-α | 0.03 ± 0.00 | 0.03 ± 0.00 | 2.83 ± 0.35* | 2.21 ± 0.37* |
| IL-1β | 0.01 ± 0.00 | 0.01 ± 0.00 | 175.18 ± 29.84* | 141.71 ± 45.16* |
| IL-6 | 0.31 ± 0.15 | 0.58 ± 0.03 | 296.38 ± 57.90* | 257.65 ± 61.94* |
| IL-10 | 0.24 ± 0.03 | 0.51 ± 0.03 | 3.44 ± 0.64* | 4.59 ± 0.45* |
| IL-12p35 | 0.01 ± 0.00 | 0.02 ± 0.00 | 142.70 ± 22.48* | 148.43 ± 5.82* |
| IL-12/23p40 | 0.15 ± 0.04 | 0.09 ± 0.02 | 377.64 ± 84.73* | 315.80 ± 27.64* |
| IL-18 | 0.39 ± 0.06 | 0.74 ± 0.07 | 3.70 ± 0.86* | 2.86 ± 0.44* |
| IL-23p19 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.90 ± 0.20* | 1.38 ± 0.05* |
| Mip-1α | 0.01 ± 0.00 | 0.01 ± 0.00 | 1.71 ± 0.27* | 1.13 ± 0.22* |
| Mcp-1 | 0.08 ± 0.02 | 0.20 ± 0.03 | 3.75 ± 0.46* | 4.14 ± 2.04* |
| TGF-β | 0.95 ± 0.22 | 0.71 ± 0.10 | 1.56 ± 0.40 | 1.67 ± 0.49 |
| MHC-II | 2.80 ± 0.34 | 2.16 ± 0.57 | 5.69 ± 0.75* | 4.62 ± 0.15* |
| Lcn2 | 1.9 ± 0.6 | 8.3 ± 1.0† | 4434.1 ± 1695.3* | 12 228.3 ± 1149.6*† |
Primary Hfe+/+ and Hfe−/− macrophages were treated with complete RPMI (control) or infected with WT Salmonella Typhimurium (S Tm) for 24 hours. Expressions of macrophage immune response genes were analyzed by quantitative RT-PCR. Specific values of target genes were normalized to those of Hprt and depicted as means ± SEM of arbitrary expression unit (AU). Values from at least 4 independent experiments were compared by Kruskal-Wallis analysis.
WT indicates wild-type; S Tm, Salmonella Typhimurium; iNOS, inducible nitric oxide synthase; TNF, tumor necrosis factor; IL, interleukin; Mip, macrophage inflammatory protein; Mcp, monocyte chemotactic protein; TGF, transforming growth factor; MHC, major histocompatibility complex; and Lcn, lipocalin.
P < .05 as compared with control cells of the corresponding genotype.
P < .05 for the comparison of Hfe+/+ and Hfe−/− phagocytes subjected to the same stimulation.