mRNA expression of the mHag-encoding genes in hematopoietic and nonhematopoietic cells
| Cell type . | MR1, % . | PTK2B, % . | LY75, % . | MTHFD1, % . |
|---|---|---|---|---|
| EBV-LCLs, n = 2 | 100 | 100 | 100 | 100 |
| PBMCs, n = 2 | 71 | 99 | 34 | 67 |
| Fibroblasts,* n = 2 | 58 | 23 | 0 | 64 |
| Keratinocytes,* n = 2 | 15 | 9 | 2 | 64 |
| Biliary epithelium,† n = 1 | 5 | 3 | 0 | 73 |
| Gut epithelium,* n = 2 | 33 | 7 | 3 | 38 |
| Proximal tubular epithelium,‡ n = 2 | 5 | 26 | 0 | 81 |
| Hepatocytes,§ n = 1 | 3 | 2 | 0 | 49 |
| Cell type . | MR1, % . | PTK2B, % . | LY75, % . | MTHFD1, % . |
|---|---|---|---|---|
| EBV-LCLs, n = 2 | 100 | 100 | 100 | 100 |
| PBMCs, n = 2 | 71 | 99 | 34 | 67 |
| Fibroblasts,* n = 2 | 58 | 23 | 0 | 64 |
| Keratinocytes,* n = 2 | 15 | 9 | 2 | 64 |
| Biliary epithelium,† n = 1 | 5 | 3 | 0 | 73 |
| Gut epithelium,* n = 2 | 33 | 7 | 3 | 38 |
| Proximal tubular epithelium,‡ n = 2 | 5 | 26 | 0 | 81 |
| Hepatocytes,§ n = 1 | 3 | 2 | 0 | 49 |
Expression of the mHag-encoding genes is corrected for expression of the PBGD housekeeping gene and shown relative to the level of gene expression in EBV-LCLs (100%). All measurements have been performed in duplicate with 100 and 10 ng cDNA.
EBV-LCLs indicates Epstein-Barr virus transformed B-cell lines ; PBMCs, peripheral blood mononuclear cells; and n, number of different samples per cell type.
Fibroblasts, keratinocytes, and gut epithelial cells were isolated from biopsies.
Biliary epithelial cells were purchased from ScienCell.
Proximal tubular epithelial cells were kindly provided by Dr C. van Kooten, Department of Nephrology, Leiden University Medical Center (LUMC).
Hepatocytes were kindly provided by Dr A. E. Kremer, Liver Center, Academic Medical Center, University of Amsterdam.