Quantitation of the c20S subunit β5 and the i20S subunit LMP7 in tumor cells
| Cell line . | Cell type . | Proteasome subunit . | ||
|---|---|---|---|---|
| β5 containing 20S, ng/μg protein (% total 20S) . | LMP7containing 20S, ng/μg protein (% total 20S) . | Total 20S, ng/μg protein . | ||
| MM1.S | Multiple myeloma | 3.9 ± 0.5 (57% ± 5%) | 2.9 ± 0.2 (45% ± 5%) | 6.8 ± 0.4 |
| RPMI-8226 | Multiple myeloma | 4.0 ± 0.5 (63% ± 2%) | 2.4 ± 0.2 (37% ± 2%) | 6.4 ± 0.7 |
| Arh77 | Plasmacytoid lymphoma-type | 4.0 ± 0.2 (54% ± 2%) | 3.4 ± 0.2 (46% ± 2%) | 7.4 ± 0.3 |
| Molt-4 | T-cell leukemia | 4.1 ± 0.6 (53% ± 3%) | 3.6 ± 0.2 (47% ± 3%) | 7.7 ± 0.7 |
| HS-Sultan | Burkitt lymphoma | 2.0 ± 0.2 (31% ± 0.7%) | 4.4 ± 0.4 (69% ± 0.7%) | 6.3 ± 0.5 |
| HT-29 | Colon adenocarcinma | 4.9 ± 0.7 (82% ± 1%) | 1.0 ± 0.1 (18% ± 1%) | 6.0 ± 0.7 |
| A549 | Lung carcinoma | 4.3 ± 0.5 (94% ± 1%) | 0.3 ± 0.02 (6% ± 1%) | 4.6 ± 0.6 |
| Cell line . | Cell type . | Proteasome subunit . | ||
|---|---|---|---|---|
| β5 containing 20S, ng/μg protein (% total 20S) . | LMP7containing 20S, ng/μg protein (% total 20S) . | Total 20S, ng/μg protein . | ||
| MM1.S | Multiple myeloma | 3.9 ± 0.5 (57% ± 5%) | 2.9 ± 0.2 (45% ± 5%) | 6.8 ± 0.4 |
| RPMI-8226 | Multiple myeloma | 4.0 ± 0.5 (63% ± 2%) | 2.4 ± 0.2 (37% ± 2%) | 6.4 ± 0.7 |
| Arh77 | Plasmacytoid lymphoma-type | 4.0 ± 0.2 (54% ± 2%) | 3.4 ± 0.2 (46% ± 2%) | 7.4 ± 0.3 |
| Molt-4 | T-cell leukemia | 4.1 ± 0.6 (53% ± 3%) | 3.6 ± 0.2 (47% ± 3%) | 7.7 ± 0.7 |
| HS-Sultan | Burkitt lymphoma | 2.0 ± 0.2 (31% ± 0.7%) | 4.4 ± 0.4 (69% ± 0.7%) | 6.3 ± 0.5 |
| HT-29 | Colon adenocarcinma | 4.9 ± 0.7 (82% ± 1%) | 1.0 ± 0.1 (18% ± 1%) | 6.0 ± 0.7 |
| A549 | Lung carcinoma | 4.3 ± 0.5 (94% ± 1%) | 0.3 ± 0.02 (6% ± 1%) | 4.6 ± 0.6 |