Hematopoietic parameters in wt and asymptomatic cmo mice
| Assay/parameter . | NA* . | IL-6 . | CSF-1 . | IL-6 and CSF-1 . | ||||
|---|---|---|---|---|---|---|---|---|
| wt . | cmo . | wt . | cmo . | wt . | cmo . | wt . | cmo . | |
| HPP-CFC (BM) | ||||||||
| Colony no., ×10−4 | 6 ± 1.4 | 8 ± 1.9 | 8 ± 3.5 | 9 ± 1.8 | 17 ± 5.1† | 24 ± 6.3† | 14 ± 4.9† | 19 ± 6.0† |
| Colony size, mm2 | 1.0 ± 0.4 | 1.0 ± 0.2 | 0.8 ± 0.3 | 1.2 ± 0.4 | 1.1 ± 0.1 | 1.5 ± 0.2§ | 0.8 ± 0.1‡ | 1.3 ± 0.1§ |
| HPP-CFC (SPL) | ||||||||
| Colony no., ×10−4 | 2 ± 0.6 | 5 ± 0.3§ | 4 ± 0.4† | 7 ± 0.9§ | 3 ± 1.2 | 8 ± 0.3†§ | 4 ± 0.9 | 10 ± 0.3†‡§ |
| Colony size, mm2 | 0.6 ± 0.14 | 1.2 ± 0.16§ | 1.2 ± 0.21† | 1.6 ± 0.04†§ | 1.4 ± 0.06† | 2.4 ± 0.03†§ | 1.5 ± 0.12† | 2.7 ± 0.32†§ |
| CFU-M (BM) | ||||||||
| Colony no., ×10−3 | 150 ± 0.6 | 130 ± 18 | 30 ± 4‡ | 50 ± 5†‡ | ||||
| Colony size, μm2 | 2661 ± 647 | 3057 ± 255 | 2590 ± 1139 | 3597 ± 579 | ||||
| CFU-M (SPL) | ||||||||
| Colony no., ×10−3 | 7 ± 1.7 | 13 ± 1.3§ | 3 ± 1.1‡ | 14 ± 1.0§ | ||||
| Colony size, μm2 | 1910 ± 405 | 6134 ± 1167§ | 9387 ± 1084‡ | 11 033 ± 1867‡ | ||||
| Macrophage proliferation (BM) doubling time, hours | 26.9 ± 0.1 | 22.2 ± 0.2§ | ||||||
| Macrophage proliferation (SPL) doubling time, hours | 42.4 ± 2.8 | 35.3 ± 1.9§ | ||||||
| Assay/parameter . | NA* . | IL-6 . | CSF-1 . | IL-6 and CSF-1 . | ||||
|---|---|---|---|---|---|---|---|---|
| wt . | cmo . | wt . | cmo . | wt . | cmo . | wt . | cmo . | |
| HPP-CFC (BM) | ||||||||
| Colony no., ×10−4 | 6 ± 1.4 | 8 ± 1.9 | 8 ± 3.5 | 9 ± 1.8 | 17 ± 5.1† | 24 ± 6.3† | 14 ± 4.9† | 19 ± 6.0† |
| Colony size, mm2 | 1.0 ± 0.4 | 1.0 ± 0.2 | 0.8 ± 0.3 | 1.2 ± 0.4 | 1.1 ± 0.1 | 1.5 ± 0.2§ | 0.8 ± 0.1‡ | 1.3 ± 0.1§ |
| HPP-CFC (SPL) | ||||||||
| Colony no., ×10−4 | 2 ± 0.6 | 5 ± 0.3§ | 4 ± 0.4† | 7 ± 0.9§ | 3 ± 1.2 | 8 ± 0.3†§ | 4 ± 0.9 | 10 ± 0.3†‡§ |
| Colony size, mm2 | 0.6 ± 0.14 | 1.2 ± 0.16§ | 1.2 ± 0.21† | 1.6 ± 0.04†§ | 1.4 ± 0.06† | 2.4 ± 0.03†§ | 1.5 ± 0.12† | 2.7 ± 0.32†§ |
| CFU-M (BM) | ||||||||
| Colony no., ×10−3 | 150 ± 0.6 | 130 ± 18 | 30 ± 4‡ | 50 ± 5†‡ | ||||
| Colony size, μm2 | 2661 ± 647 | 3057 ± 255 | 2590 ± 1139 | 3597 ± 579 | ||||
| CFU-M (SPL) | ||||||||
| Colony no., ×10−3 | 7 ± 1.7 | 13 ± 1.3§ | 3 ± 1.1‡ | 14 ± 1.0§ | ||||
| Colony size, μm2 | 1910 ± 405 | 6134 ± 1167§ | 9387 ± 1084‡ | 11 033 ± 1867‡ | ||||
| Macrophage proliferation (BM) doubling time, hours | 26.9 ± 0.1 | 22.2 ± 0.2§ | ||||||
| Macrophage proliferation (SPL) doubling time, hours | 42.4 ± 2.8 | 35.3 ± 1.9§ | ||||||
BM indicates bone marrow; CFU-M, colony-forming unit megakaryocytes; cmo, chronic multifocal osteomyelitis; CSF, colony-stimulating factor; HPP-CFC, high proliferative potential colony-forming cells; IL, interleukin; NA, no addition (absence); SPL, spleen; and wt, wild-type.
HPP-CFC assays were performed in media containing 60 ng/mL SCF and 20 ng/mL IL-3 in the absence (NA) or presence of 50 ng/mL IL-6 and/or 18 ng/mL CSF-1. CFU-M assay was performed in media containing 18 ng/mL CSF-1 ± 10 ng/mL IL-6. For proliferation assays, day 3 myeloid precursors were grown in media containing 36 ng/mL CSF-1. Doubling times were calculated exclusively for the exponential growth phase.
Different from NA; P < .05, Student t test.
Different from CSF-1; P < .05, Student t test.
Different from wt; P < .05, Student t test.