Genes differentially expressed in fetal liver cells of Zfp36l2 knockout mice
| Gene name . | Gene ID . | Function . | Microarray KO vs WT . | Northern/real-time PCR KO vs WT . |
|---|---|---|---|---|
| 3′ UTR ARE-containing | ||||
| Cxcl1 (KC) | NM_008176 | Chemokine (C-X-C motif) ligand 1 | 5.9, 3.7, 2.2 | 4,* 7† |
| Plekha3 (FAPP1) | BB780848 | Pleckstrin homology domain-containing, family A (phosphoinositide-binding specific member 3 | 1.7 | 1.6† |
| Mllt11 (Af1q) | NM_019914 | Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Dros) translocated to 11 | 1.6 | 2.2† |
| Hematopoiesis | ||||
| Il6st (gp130, CD130) | AK017211 | Interleukin 6 signal transducer | 1.79 | |
| Fga | BC005467 | Fibrinogen, α-polypeptide | 1.54 | |
| Cxcl4 (Pf4) | NM_019932 | Chemokine (C-X-C motif), ligand 4 (platelet factor 4) | −2.6 | |
| Cxcl7 (Ppbp) | NM_023785 | Chemokine (C-X-C motif), ligand 7 (pro-platelet basic protein) | −2.57 | |
| Gp5 | NM_008148 | Glycoprotein 5 (platelet) | −2.45 | |
| Gp1bb | NM_010327 | Glycoprotein Ib, β-polypeptide | −2.44 | |
| Gp1ba | NM_010326 | Glycoprotein Ib, α-polypeptide | −2.35 | |
| Itgb3 (CD61, GP3A) | AV352983 | Integrin β3 (platelet glycoprotein IIIa) | −2.29 | |
| Treml1 | AK017256 | Triggering receptor expressed on myeloid cells-like 1 | −2.24 | |
| Rab27b | BB121269 | Rab27b, member of RAS oncogene family | −2.11 | |
| Alox12 | BB554189 | Arachidonate 12-lipoxygenase | −2.05 | −1.97† |
| Mpl (TPO-R, CD110) | NM_010823 | Myeloproliferative leukemia virus oncogene (thrombopoietin receptor) | −1.92 | −2.5† |
| Itga2b (CD41, GpIIb) | NM_010575 | Integrin α2b (platelet glycoprotein IIb) | −1.78 | −2.0† |
| Thbs1 | AI385532 | Thrombospondin 1 | −1.66, −1.61 | |
| CD59a | AK005507 | CD59a antigen | −1.42 | −1.77† |
| Other | ||||
| Csf2rb | BB769628 | Colony stimulating factor 2, β, low-affinity (granulocyte-macrophage) | 2.34 | 2.0† |
| Ccr1l1 (MIP-1 alphaRL1) | NM_007718 | Chemokine (C-C motif) receptor 1-like 1 (macrophage inflammatory protein-1 alpha receptor-like 1) | −2.4 | |
| HSCs | ||||
| Vwf | BB667216 | von Willebrand factor homolog | −1.92‡ | |
| Slamf1 (Slam, CD150) | BB132695 | Signaling lymphocytic activation molecule family member 1 | −1.83 | |
| Pbx1 | BG073018 | Pre-B-cell leukemia transcription factor | −1.62§ |
| Gene name . | Gene ID . | Function . | Microarray KO vs WT . | Northern/real-time PCR KO vs WT . |
|---|---|---|---|---|
| 3′ UTR ARE-containing | ||||
| Cxcl1 (KC) | NM_008176 | Chemokine (C-X-C motif) ligand 1 | 5.9, 3.7, 2.2 | 4,* 7† |
| Plekha3 (FAPP1) | BB780848 | Pleckstrin homology domain-containing, family A (phosphoinositide-binding specific member 3 | 1.7 | 1.6† |
| Mllt11 (Af1q) | NM_019914 | Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Dros) translocated to 11 | 1.6 | 2.2† |
| Hematopoiesis | ||||
| Il6st (gp130, CD130) | AK017211 | Interleukin 6 signal transducer | 1.79 | |
| Fga | BC005467 | Fibrinogen, α-polypeptide | 1.54 | |
| Cxcl4 (Pf4) | NM_019932 | Chemokine (C-X-C motif), ligand 4 (platelet factor 4) | −2.6 | |
| Cxcl7 (Ppbp) | NM_023785 | Chemokine (C-X-C motif), ligand 7 (pro-platelet basic protein) | −2.57 | |
| Gp5 | NM_008148 | Glycoprotein 5 (platelet) | −2.45 | |
| Gp1bb | NM_010327 | Glycoprotein Ib, β-polypeptide | −2.44 | |
| Gp1ba | NM_010326 | Glycoprotein Ib, α-polypeptide | −2.35 | |
| Itgb3 (CD61, GP3A) | AV352983 | Integrin β3 (platelet glycoprotein IIIa) | −2.29 | |
| Treml1 | AK017256 | Triggering receptor expressed on myeloid cells-like 1 | −2.24 | |
| Rab27b | BB121269 | Rab27b, member of RAS oncogene family | −2.11 | |
| Alox12 | BB554189 | Arachidonate 12-lipoxygenase | −2.05 | −1.97† |
| Mpl (TPO-R, CD110) | NM_010823 | Myeloproliferative leukemia virus oncogene (thrombopoietin receptor) | −1.92 | −2.5† |
| Itga2b (CD41, GpIIb) | NM_010575 | Integrin α2b (platelet glycoprotein IIb) | −1.78 | −2.0† |
| Thbs1 | AI385532 | Thrombospondin 1 | −1.66, −1.61 | |
| CD59a | AK005507 | CD59a antigen | −1.42 | −1.77† |
| Other | ||||
| Csf2rb | BB769628 | Colony stimulating factor 2, β, low-affinity (granulocyte-macrophage) | 2.34 | 2.0† |
| Ccr1l1 (MIP-1 alphaRL1) | NM_007718 | Chemokine (C-C motif) receptor 1-like 1 (macrophage inflammatory protein-1 alpha receptor-like 1) | −2.4 | |
| HSCs | ||||
| Vwf | BB667216 | von Willebrand factor homolog | −1.92‡ | |
| Slamf1 (Slam, CD150) | BB132695 | Signaling lymphocytic activation molecule family member 1 | −1.83 | |
| Pbx1 | BG073018 | Pre-B-cell leukemia transcription factor | −1.62§ |
Partial list of genes differentially expressed in Zfp36l2 KO fetal liver at a significance level of P < .001. Total RNA was isolated from 5 KO and 5 WT E14.5 fetal livers and used for gene expression analysis using Affymetrix Mouse Genome 2.0 GeneChip Arrays (Mouse 430, Version 2). Values represent the fold changes (positive numbers indicate increased expression and negative numbers indicate decreased expression) in gene expression in KO fetal liver compared with WT fetal liver.
Microarray mRNA changes confirmed by real-time PCR.
Microarray mRNA changes confirmed by Northern analysis.
P = .003.
P = .02.