Methods to detect minimal residual disease in CML
| Method . | Target . | Sensitivity, percentage . | Advantages . | Disadvantages . |
|---|---|---|---|---|
| Morphology | Cellular morphology | 5 | Standard | Poor sensitivity |
| Cytogenetics | Chromosome structure | 1-5 | Widely available | Low sensitivity, bone marrow only |
| FISH | Specific genetic marker(s) | 0.1-5 | Fast (1-2 days) | Does not look for other clonal events |
| QPCR | RNA sequence | 0.001-0.01 | Very sensitive | Poor standardization, laboratory-intensive |
| Method . | Target . | Sensitivity, percentage . | Advantages . | Disadvantages . |
|---|---|---|---|---|
| Morphology | Cellular morphology | 5 | Standard | Poor sensitivity |
| Cytogenetics | Chromosome structure | 1-5 | Widely available | Low sensitivity, bone marrow only |
| FISH | Specific genetic marker(s) | 0.1-5 | Fast (1-2 days) | Does not look for other clonal events |
| QPCR | RNA sequence | 0.001-0.01 | Very sensitive | Poor standardization, laboratory-intensive |