Selected genes up-regulated by HLX in endothelial cells
| UniGene ID . | Entrez gene . | Gene symbol . | Gene title . | Ad.HLX/Ad.con . | |||
|---|---|---|---|---|---|---|---|
| 4 h . | 8 h . | 16 h . | 32 h . | ||||
| Surface receptors | |||||||
| Hs.585457 | 219699 | UNC5B | unc-5 homolog B (C elegans) | 1.41 | 2.23 | 5.71 | 7.14 |
| Hs.432329 | 5361 | PLXNA1 | Plexin A1 | 1.15 | 2.96 | 4.61 | 8.38 |
| Hs.132781 | 9466 | IL27RA | Interleukin 27 receptor, alpha | 0.91 | 1.86 | 4.18 | 5.53 |
| Secreted proteins | |||||||
| Hs.9315 | 56944 | OLFML3 | Olfactomedin-like 3 | 1.09 | 1.72 | 4.62 | 23.68 |
| Hs.59729 | 56920 | SEMA3G | Sema domain, immunoglobulin domain, short basic domain, secreted (semaphorin) 3G | 1.27 | 2.28 | 2.88 | 4.89 |
| Cytoplasmic (signaling) proteins | |||||||
| Hs.417050 | 8900 | CCNA1 | Cyclin A1 | 0.78 | 2.15 | 3.79 | 2.96 |
| Hs.2128 | 1847 | DUSP5 | Dual-specificity phosphatase 5 | 1.07 | 2.38 | 3.76 | 3.54 |
| Hs.381167 | 1992 | SERPINB1 | Serpin peptidase inhibitor, clade B (ovalbumin), member 1 | 0.93 | 1.33 | 3.15 | 4.25 |
| Transcription factors | |||||||
| Hs.360174 | 6591 | SNAI2 | Snail homolog 2 (Drosophila) | 1.58 | 3.87 | 4.51 | 6.91 |
| Hs.525704 | 3725 | JUN | Jun oncogene | 1.48 | 1.51 | 4.06 | 3.14 |
| Hs.250666 | 3280 | HES1 | Hairy and enhancer of split 1 (Drosophila) | 2.13 | 3.63 | 3.49 | 4.37 |
| Hs.659681 | 54880 | BCOR | BCL6 corepressor | 2.01 | 2.54 | 3.45 | 5.37 |
| UniGene ID . | Entrez gene . | Gene symbol . | Gene title . | Ad.HLX/Ad.con . | |||
|---|---|---|---|---|---|---|---|
| 4 h . | 8 h . | 16 h . | 32 h . | ||||
| Surface receptors | |||||||
| Hs.585457 | 219699 | UNC5B | unc-5 homolog B (C elegans) | 1.41 | 2.23 | 5.71 | 7.14 |
| Hs.432329 | 5361 | PLXNA1 | Plexin A1 | 1.15 | 2.96 | 4.61 | 8.38 |
| Hs.132781 | 9466 | IL27RA | Interleukin 27 receptor, alpha | 0.91 | 1.86 | 4.18 | 5.53 |
| Secreted proteins | |||||||
| Hs.9315 | 56944 | OLFML3 | Olfactomedin-like 3 | 1.09 | 1.72 | 4.62 | 23.68 |
| Hs.59729 | 56920 | SEMA3G | Sema domain, immunoglobulin domain, short basic domain, secreted (semaphorin) 3G | 1.27 | 2.28 | 2.88 | 4.89 |
| Cytoplasmic (signaling) proteins | |||||||
| Hs.417050 | 8900 | CCNA1 | Cyclin A1 | 0.78 | 2.15 | 3.79 | 2.96 |
| Hs.2128 | 1847 | DUSP5 | Dual-specificity phosphatase 5 | 1.07 | 2.38 | 3.76 | 3.54 |
| Hs.381167 | 1992 | SERPINB1 | Serpin peptidase inhibitor, clade B (ovalbumin), member 1 | 0.93 | 1.33 | 3.15 | 4.25 |
| Transcription factors | |||||||
| Hs.360174 | 6591 | SNAI2 | Snail homolog 2 (Drosophila) | 1.58 | 3.87 | 4.51 | 6.91 |
| Hs.525704 | 3725 | JUN | Jun oncogene | 1.48 | 1.51 | 4.06 | 3.14 |
| Hs.250666 | 3280 | HES1 | Hairy and enhancer of split 1 (Drosophila) | 2.13 | 3.63 | 3.49 | 4.37 |
| Hs.659681 | 54880 | BCOR | BCL6 corepressor | 2.01 | 2.54 | 3.45 | 5.37 |
Total RNA was isolated from HUVECs after infection with HLX-encoding (Ad.HLX) and control adenoviruses (Ad.con) for 4, 8, 16, and 32 hours. RNA was subjected to microarray analysis using the Affymetrix Human Genome U133 Plus 2.0 Array. Changes in gene expression induced in Ad.HLX relative to Ad.con-infected cultures are displayed as fold-induction at the given time points. The full set of Affymetrix data is available in the GEO database under GSE13054.