Mascot search parameters for COFRADIC approaches
| . | Fixed modification . | Variable modification . |
|---|---|---|
| N-terminal COFRADIC (protease: Arg-C/P) | Oxidation to sulphoxide (M), trideutero-acetylation (K), carbamidomethylation (C) | Pyroglutamate (N-term Q), pyrocarbamidomethyl cysteine (N-term alkylated C), acetylation (N-term), trideutero-acetlyation (N-term) |
| Met-/Cys-COFRADIC (protease: trypsin) | Oxidation to sulphoxide (M), carbamidomethylation (C) | Pyroglutamate (N-term Q), pyrocarbamidomethyl cysteine (N-term alkylated C), acetylation (N-term) |
| . | Fixed modification . | Variable modification . |
|---|---|---|
| N-terminal COFRADIC (protease: Arg-C/P) | Oxidation to sulphoxide (M), trideutero-acetylation (K), carbamidomethylation (C) | Pyroglutamate (N-term Q), pyrocarbamidomethyl cysteine (N-term alkylated C), acetylation (N-term), trideutero-acetlyation (N-term) |
| Met-/Cys-COFRADIC (protease: trypsin) | Oxidation to sulphoxide (M), carbamidomethylation (C) | Pyroglutamate (N-term Q), pyrocarbamidomethyl cysteine (N-term alkylated C), acetylation (N-term) |