Table 1 Frequency, cloning efficiency,... | American Society of Hematology

Table 1

Frequency, cloning efficiency, and GFP expression of stem/progenitor cells, immature (CD71^high) and mature (CD71^med) erythroid cells, and MKs prospectively isolated from marrow and spleen of ^{−2.7kbGata1}GFPGata1^+/0 and ^−2.7kbGata1GFPGata1^low/0 mice

	^{−2.7kbGata1}GFPGata1^+/0						^{−2.7kbGata1}GFPGata1^low/0
	Frequency	Cloning efficiency	GFP^neg		GFP^pos		Frequency	Cloning efficiency	GFP^neg		GFP^pos
	Frequency	Cloning efficiency	%	AFI	%	AFI	Frequency	Cloning efficiency	%	AFI	%	AFI
Bone marrow
Stem/progenitor cells
CMP/GMP (cKit^pos/CD34^pos)	2.1	59.5 ± 5.4	90	40	9	110	2.1	22.8 ± 5.2*	82	90	18	900
Stem/MEP (cKit^pos/CD34^neg)	2.7	13.6 ± 5.2	58	35	42	1200	2.8	0.1 ± 0.05*	89	65	11	150
Erythroblasts
CD71^high	8.8	na	8	bd	92	1632	4.3	na	0	bd	100	1695
CD71^med	10.2	na	2	bd	98	555	1.8	na	2	bd	98	780
Megakaryocytes
CD61^posCD41^pos	3.8	na	100	20	0	bd	26.8	na	99	20	1	bd
Spleen
Stem/progenitor cells
CMP/GMP (cKit^pos/CD34^pos)	2.2	0.2 ±0.1	63	20	36	1000	2.4	72.3 ± 28.4*	17	98	40	370
											43	10 100
Stem/MEP (cKit^pos/CD34^neg)	0.6	0	67	40	33	130	1.3	18.2 ± 8.9*	10	80	47	230
											43	12 200
Erythroblasts
CD71^high	1.2	na	4	bd	96	601	2.4	na	0	bd	100	1960
CD71^med	1.4	na	2	bd	98	391	1.4	na	1	bd	99	860
Megakaryocytes
CD61^posCD41^pos	4.5	na	98	20	1.6	bd	26.3	na	3	bd	97	227

	^{−2.7kbGata1}GFPGata1^+/0						^{−2.7kbGata1}GFPGata1^low/0
	Frequency	Cloning efficiency	GFP^neg		GFP^pos		Frequency	Cloning efficiency	GFP^neg		GFP^pos
	Frequency	Cloning efficiency	%	AFI	%	AFI	Frequency	Cloning efficiency	%	AFI	%	AFI
Bone marrow
Stem/progenitor cells
CMP/GMP (cKit^pos/CD34^pos)	2.1	59.5 ± 5.4	90	40	9	110	2.1	22.8 ± 5.2*	82	90	18	900
Stem/MEP (cKit^pos/CD34^neg)	2.7	13.6 ± 5.2	58	35	42	1200	2.8	0.1 ± 0.05*	89	65	11	150
Erythroblasts
CD71^high	8.8	na	8	bd	92	1632	4.3	na	0	bd	100	1695
CD71^med	10.2	na	2	bd	98	555	1.8	na	2	bd	98	780
Megakaryocytes
CD61^posCD41^pos	3.8	na	100	20	0	bd	26.8	na	99	20	1	bd
Spleen
Stem/progenitor cells
CMP/GMP (cKit^pos/CD34^pos)	2.2	0.2 ±0.1	63	20	36	1000	2.4	72.3 ± 28.4*	17	98	40	370
											43	10 100
Stem/MEP (cKit^pos/CD34^neg)	0.6	0	67	40	33	130	1.3	18.2 ± 8.9*	10	80	47	230
											43	12 200
Erythroblasts
CD71^high	1.2	na	4	bd	96	601	2.4	na	0	bd	100	1960
CD71^med	1.4	na	2	bd	98	391	1.4	na	1	bd	99	860
Megakaryocytes
CD61^posCD41^pos	4.5	na	98	20	1.6	bd	26.3	na	3	bd	97	227

The frequency of stem/progenitor cells is expressed as the percentage of the total mononuclear cells analyzed. The frequency of GFP^pos cells is expressed as the percentage of the cells within the corresponding gate. Flow cytometry data correspond to the determinations presented in Figure 4. Similar results were observed in 3 to 4 additional mice per experimental point. Cloning efficiency is reported as the number of day-15 colonies observed in cultures seeded with 100 purified cells and is expressed as mean (± SD) in at least 3 separate determinations per experimental point.

AFI indicates average fluorescence intensity; na, not applicable; and bd, below detection.

Values observed in Gata1^low mice statistically different (P < .01) from those observed in mice wild-type at the Gata1 locus.

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