Primers for quantitative real-time PCR
| Primer . | Sequence, 5′ → 3′ . |
|---|---|
| Mouse HIF-1α forward | GAAATGGCCCAGTGAGAAAA |
| Mouse HIF-1α-exon2 forward | GGCGAAGCAAAGAGTCTGAAG |
| Mouse HIF-1α reverse | CTTCCACGTTGCTGACTTGA |
| Mouse HIF-2α forward | CCTGCAGCCTCAGTGTATCA |
| Mouse HIF-2α reverse | GTGTGGCTTGAACAGGGATT |
| Mouse VEGF forward | TGTCACCACCACGCCATCA |
| Mouse VEGF reverse | GGAATCCCAGAAACAACCCTAATC |
| Mouse Glut-1 forward | GAGGAGCTCTTCCACCCTCT |
| Mouse Glut-1 reverse | TCCTCCTGGACTTCACTGCT |
| Mouse ribosomal 16S protein forward | AGATGATCGAGCCGCGC |
| Mouse ribosomal 16S protein reverse | GCTACCAGGGCCTTTGAGATGGA |
| Human HIF-1α forward | CTCAAAGTCGGACAGCCTCA |
| Human HIF-1α reverse | CCCTGCAGTAGGTTTCTGCT |
| Human actin forward | TGACGGGGTCACCCACACTGTGCCCATCTA |
| Human actin reverse | CTAGAAGCATTTGCGGTCGACGATGGAGGG |
| Primer . | Sequence, 5′ → 3′ . |
|---|---|
| Mouse HIF-1α forward | GAAATGGCCCAGTGAGAAAA |
| Mouse HIF-1α-exon2 forward | GGCGAAGCAAAGAGTCTGAAG |
| Mouse HIF-1α reverse | CTTCCACGTTGCTGACTTGA |
| Mouse HIF-2α forward | CCTGCAGCCTCAGTGTATCA |
| Mouse HIF-2α reverse | GTGTGGCTTGAACAGGGATT |
| Mouse VEGF forward | TGTCACCACCACGCCATCA |
| Mouse VEGF reverse | GGAATCCCAGAAACAACCCTAATC |
| Mouse Glut-1 forward | GAGGAGCTCTTCCACCCTCT |
| Mouse Glut-1 reverse | TCCTCCTGGACTTCACTGCT |
| Mouse ribosomal 16S protein forward | AGATGATCGAGCCGCGC |
| Mouse ribosomal 16S protein reverse | GCTACCAGGGCCTTTGAGATGGA |
| Human HIF-1α forward | CTCAAAGTCGGACAGCCTCA |
| Human HIF-1α reverse | CCCTGCAGTAGGTTTCTGCT |
| Human actin forward | TGACGGGGTCACCCACACTGTGCCCATCTA |
| Human actin reverse | CTAGAAGCATTTGCGGTCGACGATGGAGGG |