Mutation-specific forward primers for quantitative detection of the mutated NPM1 allele
| Name . | Sequence . | Sensitivity . | Specificity . |
|---|---|---|---|
| Q-NPM1-F_Mut_A | TCAAGATCTCTGTCTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_B | TCAAGATCTCTGCATG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_D | TCAAGATCTCTGCCTG | 1:10 000 | No |
| Q-NPM1-F_Mut_H | TCAAGATCTCTGAGGA | 1:10 000 | No |
| Q-NPM1-F_Mut_I | TCAAGATCTCTGCTTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_L | TCAAGATCTCTGTTTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_K | TCAAGATCTCTGCCAG | 1:10 000 | No |
| Q-NPM1-F_Mut_N | TCAAGATCTCTGTCGG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_R | TCAAGATCTCTGTATG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_S | TGGCAGTGTTTTTCTC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_T | TGGCAGTGCATGGCTC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_X | TCAAGATCTCTGTTCC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_Y | TCAAGATCTCTGCCGA | 1:100 000 | Yes |
| Q-NPM1-F_Mut_Z | TCAAGATCTCTGTAGG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZD | GATCTCTGGCAGAGGC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZF | GATCTCTGGCAGCTTCTCCA | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZG | CTCTGGCAGCGGATGGC | 1:10 000 | No |
| Name . | Sequence . | Sensitivity . | Specificity . |
|---|---|---|---|
| Q-NPM1-F_Mut_A | TCAAGATCTCTGTCTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_B | TCAAGATCTCTGCATG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_D | TCAAGATCTCTGCCTG | 1:10 000 | No |
| Q-NPM1-F_Mut_H | TCAAGATCTCTGAGGA | 1:10 000 | No |
| Q-NPM1-F_Mut_I | TCAAGATCTCTGCTTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_L | TCAAGATCTCTGTTTG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_K | TCAAGATCTCTGCCAG | 1:10 000 | No |
| Q-NPM1-F_Mut_N | TCAAGATCTCTGTCGG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_R | TCAAGATCTCTGTATG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_S | TGGCAGTGTTTTTCTC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_T | TGGCAGTGCATGGCTC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_X | TCAAGATCTCTGTTCC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_Y | TCAAGATCTCTGCCGA | 1:100 000 | Yes |
| Q-NPM1-F_Mut_Z | TCAAGATCTCTGTAGG | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZD | GATCTCTGGCAGAGGC | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZF | GATCTCTGGCAGCTTCTCCA | 1:100 000 | Yes |
| Q-NPM1-F_Mut_ZG | CTCTGGCAGCGGATGGC | 1:10 000 | No |
Names are according to laboratory-specific names of individual mutation. Sequences in bold are the sequences of the mutations.