Induction of telomerase activity in normal bone marrow CD34+ cells by methyltrienolone (R1881)
| Donor . | Relative telomerase activity R1881 concentration . | ||
|---|---|---|---|
| 0 . | 0.5 μM . | 5 μM . | |
| 1 | 100% ± 5.9% | 142% ± 7.1% | 133% ± 11.8% |
| 2 | 100% ± 5.7% | 152% ± 24% | 139% ± 14% |
| 3 | 100% ± 5.6% | 142% ± 19% | 134% ± 13% |
| 4 | 100% ± 5.8% | 132% ± 6% | 146% ± 0.9% |
| 5 | 100% ± 5.5% | 116% ± 9.9% | 139% ± 2.1% |
| Average | 100% ± 2.3% | 137% ± 6.1%* | 138% ± .3%* |
| Donor . | Relative telomerase activity R1881 concentration . | ||
|---|---|---|---|
| 0 . | 0.5 μM . | 5 μM . | |
| 1 | 100% ± 5.9% | 142% ± 7.1% | 133% ± 11.8% |
| 2 | 100% ± 5.7% | 152% ± 24% | 139% ± 14% |
| 3 | 100% ± 5.6% | 142% ± 19% | 134% ± 13% |
| 4 | 100% ± 5.8% | 132% ± 6% | 146% ± 0.9% |
| 5 | 100% ± 5.5% | 116% ± 9.9% | 139% ± 2.1% |
| Average | 100% ± 2.3% | 137% ± 6.1%* | 138% ± .3%* |
Telomerase activity of cultured CD34+ cells is expressed as relative telomerase activity (considering telomerase activity of each donor in the absence of hormones as reference activity). CD34+ bone marrow cells from 3 healthy donors were purified and cultured for 8 days in liquid media in the presence of methyltrienolone.
P < .01, Kruskal Wallis test; P < .05 for each column in comparison to “0” (no androgen) using the Dunn multiple comparison test.