Relative expression of genes encoding immune-related cell surface receptors in H-mDCs versus mDCs*
| GenBank accession no. . | Gene symbol . | Full name . | Main function(s) of gene product . | Fold increase‡ . | HRE§ . |
|---|---|---|---|---|---|
| Pattern recognition/scavenger receptors | |||||
| NM_005582 | CD180 (RP105)† | CD180 antigen | Belongs to the TLR family; negative regulator of TLR4 signaling on myeloid and B cells and of innate immune responses to pathogens and danger; NF-κB activator | 13.5 | − |
| NM_012072 | CD93 (C1qR1)† | Complement component 1q, receptor 1 | Defense collagen receptor for C1q, mannose-binding lectin, and surfactant protein A; mainly expressed on monocytes, neutrophils, and endothelial cells; modulates phagocytosis and clearance of apoptotic cells; mediates leukocyte-endothelial cell interactions | 8.6 | − |
| NM_003264 | TLR2† | Toll-like receptor 2 | Member of the TLR family expressed on cells of the innate immune system; plays an essential role in PAMP recognition leading to the generation of immune responses; implicated in the pathogenesis of autoimmune and inflammatory diseases | 7.7 | + |
| AW299226 | CD36† | Thrombospondin receptor | Multifunctional class B scavenger receptor; involved in the uptake of oxidized LDL, collagen, thrombospondin, anionic phospholipids, and apoptotic cells; adhesion molecule | 5 | + |
| BC005254 | CLEC2B† | C-type lectin domain family 2, member B | Member of the NK cell C-type lectin-like receptor family; platelet activation receptor; involved in tumor cell growth/metastatization | 4.8 | − |
| BE676623 | CD71† | Transferrin receptor | Regulator of cellular uptake of iron and iron metabolism; IgA receptor expressed on DCs | 4.5 | − |
| NM_001877 | CD21 (CR2)† | Complement component receptor 2 | EBV receptor overexpressed on B cells and follicular DCs; required for generation of immune responses to T cell antigens | 4.4 | − |
| AF285089 | CLEC2D† | C-type lectin domain family 2, member D | Member of the NK cell C-type lectin receptor family; pattern recognition receptor expressed on myeloid cells; mediates phagocytosis and proinflammatory cytokine production; regulates NK and APC cross-talk | 4.1 | + |
| NM_006770 | MARCO† | Macrophage receptor with collagenous structure | Member of the class A scavenger receptor family; pattern recognition receptor up-regulated on phagocytes in response to TLR agonists and Gram+/− bacteria; implicated in phagocytic activity and proinflammatory cytokine production; plays a role in host defense to pathogens, cell-cell recognition, and modified LDL scavenging | 3.7 | − |
| AL050262 | TLR1† | Toll-like receptor 1 | Member of the TLR family expressed on cells of the innate immune system; plays a fundamental role in PAMP recognition; mediates production of cytokines for the activation of innate immunity | 3.6 | + |
| AF313468 | CLEC7A | C-type lectin domain family 7, member A | Member of the C-type lectin-like receptor family; pattern recognition receptor for fungal β-glucan expressed on myeloid cells including DCs; activates phagocytosis, ROS and cytokine production, DC maturation, and T-cell costimulatory activity | 2.9 | + |
| NM_018690 | APOB48R | Apolipoprotein B48 receptor | Binds to apolipoprotein B48 on reticuloendothelial cells and regulates lipid transport into the cell | 2.9 | + |
| NM_000573 | CD35 (C3b/4bR1) | Complement component 3b/4b, receptor 1 | Member of the complement activation receptor (RCA) family expressed on follicular DCs and other leukocytes; mediates binding of complement-bound opsonins and immune complexes; enhances adaptive immune responses | 2.6 | − |
| U81501 | FPRL1 | Formyl peptide receptor-like 1 | High affinity receptor for bacteria-derived and host-derived N-formyl-methionyl peptides; key player in innate immunity and host defense against pathogen infection; mediates phagocyte chemotactic migration and IL-1 production | 2.6 | + |
| M74921 | EDNRB | Endothelin receptor type B | G protein-coupled receptor for the vasoactive peptide, endothelin; functions as a clearance receptor contributing to the regulation of vascular homeostasis | 2.2 | + |
| Costimulatory and adhesion/homing molecules | |||||
| 201666_at | TIMP1 | TIMP metallopeptidase inhibitor 1 | Matrix metalloproteinase inhibitor; promotes proliferation of several cell types and has an antiapoptotic function | 15.1 | + |
| NM_000889 | ITGB7 | Integrin, β 7 | Receptor for VCAM1 and fibronectin; interacts with MADCAM1 adhesion molecule expressed on vascular endothelium; plays a role in cell adhesive interactions | 8.3 | − |
| AK025016 | CD9† | CD9 antigen | Member of the tetraspanin family; facilitates the organization of multimolecular membrane complexes, including integrins; plays a role in leukocyte activation, differentiation, and migration | 8 | + |
| N95414 | ITGA2 (CD49B) | Integrin, α 2 | Receptor for laminin, collagen, fibronectin, and E-cadherin; involved in cell adhesion, cell surface-mediated signaling, extracellular matrix generation, and organization | 6.9 | + |
| AI653981 | L1CAM (CD171) | L1 cell adhesion molecule | Costimulatory/adhesion molecule belonging to the Ig superfamily; regulates DC transendothelial migration and trafficking; stimulates cancer cell motility and spreading | 6.7 | + |
| AF280547 | NRP1 | Neuropilin 1 | Coreceptor of tyrosine kinase receptors for vascular endothelial growth factor, class 3 semaphorins, and placenta growth factor; involved in the regulation of cell growth and migration, angiogenesis, DC and T-cell interaction, and in the initiation of primary immune responses | 5.8 | + |
| NM_001109 | ADAM8 (CD156) | ADAM metallopeptidase domain 8 | Membrane-anchored protein implicated in cell-cell and cell-matrix interactions | 4.5 | + |
| AF213460 | EPHA3 | Ephrin receptor A3 | Receptor for members of the ephrin-A family of receptor tyrosine kinases; mediates cell-cell contact, cellular adhesion, angiogenesis, and developmental remodeling | 4 | − |
| X74039 | CD87 | Plasminogen activator, urokinase receptor | Receptor for urokinase plasminogen activator; regulates cell-surface plasminogen activation; mediates cell adhesion by binding to vitronectin; modulates the proliferative, adhesive, and migratory cell phenotype | 3.8 | − |
| NM_013447 | CD312 (MR2) | EGF-like domain containing hormone receptor-like 2 | Member of the GPCR family of adhesion molecules differentially expressed during myeloid cell differentiation, maturation, and activation; plays a role in migration and adhesion | 3.8 | − |
| NM_000560 | CD53 | CD53 antigen | Member of the tetraspanin family; complexes with integrins and plays a role in the regulation of leukocyte activation, growth, and motility; contributes to the transduction of CD2-generated signals in T and NK cells | 3.6 | − |
| NM_002205 | ITGA5 (CD49) | Integrin, α 5 | Receptor for fibronectin and fibrinogen; involved in cell adhesion to fibronectin and cell surface mediated signaling | 3.6 | + |
| NM_002414 | CD99 | CD99 antigen | Mediates leukocyte adhesion amd transendothelial migration | 3.4 | + |
| BE870509 | MET (HGFR)† | Met proto-oncogene (hepatocyte growth factor receptor) | Widely expressed proto-oncogene encoding the tyrosine-kinase receptor for hepatocyte growth factor and scatter factor; plays a role in cell growth and motility in many physiologic and pathologic processes | 3.4 | + |
| M37780 | PECAM-1 (CD31)† | Platelet/endothelial cell adhesion molecule | Cell adhesion molecule predominantly expressed on endothelial cells | 3 | + |
| AW131039 | ITGB8 | Integrin, β 8 | Receptor for fibronectin; mediates cell-cell and cell-extracellular matrix interaction | 3 | + |
| NM_000655 | LECAM1 | Lymphocyte adhesion molecule 1 (selectin L) | Member of a family of adhesion/homing receptors required for leukocyte binding and rolling on endothelial cells; facilitates leukocyte migration into secondary lymphoid organs and inflammatory sites | 3 | − |
| M80436 | PAFR | Platelet-activating factor receptor | G protein-coupled receptor for platelet-activating factor with pleiotropic functions, including regulation of cell motility, smooth muscle contraction, cytokine synthesis, and release; implicated in allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes | 2.8 | − |
| NM_001778 | CD48† | B-cell membrane protein | Activation-associated glycoprotein, member of the Ig superfamily; binds CD2 and regulates NK and T-cell activation | 2.7 | + |
| U94903 | CD44† | CD44 antigen | Receptor for hyaluronic acid, osteopontin, collagens, and matrix metalloproteinases; involved in cell-cell interaction, leukocyte adhesion, recirculation and homing, hematopoiesis, and tumor spreading | 2.7 | − |
| U87967 | CD39 | Ectonucleoside triphosphate diphosphohydrolase | Surface enzyme involved in the reduction of inflammatory cell adhesion and trafficking and tissue injury; contributes to vascular homeostasis regulation | 2.7 | − |
| D37781 | CD148† | Protein tyrosine phosphatase, receptor type, J | Receptor PTP induced in macrophages by proinflammatory stimuli; regulates cell spreading, cytoskeletal rearrangements, and chemotaxis | 2.3 | + |
| AJ277151 | CD134† | Tumor necrosis factor receptor superfamily, member 4 | Regulates T-cell expansion and survival, NK cell functions, and cross-talk with APCs; NF-κB activator | 2.1 | − |
| S78505 | PRLR | Prolactin receptor | Widespread receptor, major mediator of prolactin effects on cell proliferation, survival, cytoskeletal modification and differentiation | 2.1 | + |
| NM_002207 | ITGA9 | Integrin, α 9 | Receptor for VCAM1, tenascin, and osteopontin; participates in cell adhesion and motility | 2 | − |
| Immunoregulatory receptors | |||||
| U43677 | FCAR (CD89) | Fc fragment of IgA, receptor | ITAM-bearing receptor of the Fc region of IgA expressed on myeloid cells; mediates both pro- and anti-inflammatory functions of IgA | 55 | + |
| NM_018643 | TREM-1† | Triggering receptor expressed on myeloid cells 1 | Ig superfamily immunoregulatory receptor expressed on monocytes/macrophages and neutrophils; stimulates their proinflammatory functions and acts as an inflammatory amplifier via functional interaction with TLRs; involved in inflammatory diseases and septic shock | 39.8 | + |
| NM_001432 | EREG† | Epiregulin | Member of the epidermal growth factor (EGF) family; binds EGFR and members of ErbB tyrosine-kinase receptor family; mediates cell proliferation and proinflammatory cytokine production; promotes wound healing | 20.8 | + |
| NM_033438 | SLAMF9† | SLAM family member 9 | Member of the signaling leukocyte activation Ig subfamily; involved in innate and acquired immune responses through the regulation of multiple hematopoietic cell functions, including DC maturation | 12.2 | + |
| AW119113 | BDCA3 (CD141)† | Blood dendritic cell Ag (thrombomodulin) | Expressed on DCs; associated with TH2-skewed immune responses; plays a pathogenetic role in atopy and asthma | 11.4 | − |
| U90940 | FCGR2C (CD32) | Fc fragment of IgG, low affinity IIc, receptor | Involved in myeloid cell effector and regulatory functions, such as phagocytosis of immune complexes and modulation of antibody production by B cells | 11 | − |
| AI246590 | IRAK-2 | Interleukin-1 receptor-associated kinase 2 | IRAK kinase family member; key component of IL-1R and TLR signal transduction pathways; involved in the regulation of intracellular signaling networks controlling inflammation | 9.7 | + |
| NM_000570 | FCGR3A (CD16) | Fc fragment of IgG, low affinity IIIa, receptor | Type 1 transmembrane glycoprotein of the Ig superfamily expressed on several immune cells, including phagocytes; activating receptor involved in phagocytosis, inflammatory mediator release, ADCC, and immune complex clearance | 9.6 | + |
| AJ130712 | SIGLEC7 | Sialic acid binding Ig-like lectin 7 | ITIM-containing member of the CD33-related Siglec receptor family, weakly expressed on monocytic cells; acts as an inhibitory receptor | 8 | − |
| NM_006866 | LILRA2 (ILT1)† | Leukocyte immunoglobulin-like receptor A2 | Member of the leukocyte Ig-like immunoreceptor family, selectively expressed on myeloid cells, including DCs, and inflammatory tissues; acts as an activating receptor pairing with FcγR for signaling; promotes inflammatory responses | 6.4 | + |
| 1557444_at | TREML3† | Triggering receptor expressed on myeloid cells-like 3 | Ig superfamily immunoregulatory receptor expressed on myeloid cells, structurally related to the TREM gene family | 5.9 | − |
| AW241983 | CD307 | Fc receptor-like 5 | Differentiation antigen of B-lineage cells; plays an immunoregulatory role | 5 | − |
| NM_001772 | CD33 | CD33 antigen | Marker of myeloid cell differentiation belonging to the sialic acid-binding Ig-like lectin (Siglec) family; negative regulator of myeloid cell activation and DC generation | 4.7 | − |
| AK026133 | SEMA4B† | Semaphorin 4B | Integral membrane protein with an Ig extracellular domain, widely expressed throughout the nervous system; provides axonal guidance cues during neuronal development | 4.1 | − |
| NM_000956 | PTGER2 | Prostaglandin E receptor 2 | Receptor for prostaglandin E2, expressed on macrophages and mDCs; activates the expression of multiple genes implicated in the regulation of immunity and inflammation; involved in CCR7-mediated mDC migration | 3.7 | − |
| NM_004106 | FCER1G | Fc fragment of IgE, high affinity I, receptor | ITAM-containing subunit of the high-affinity IgE receptor and of other Fc receptors; expressed on mast cells and APCs; involved in allergic reactions mediated by IgE and in the regulation of immune responses | 3.6 | + |
| AF025529 | LILRA1 (LIR6) | Leukocyte immunoglobulin-like receptor A1 | Member of the leukocyte Ig-like immunoreceptor family expressed predominantly on monocytes/macrophages and B cells and at lower levels on DCs; associates with FcγR and acts as receptor for MHCI antigens | 3.5 | + |
| NM_006378 | SEMA4D (CD100)† | Semaphorin 4D | Integral membrane protein with an Ig extracellular domain; controls proliferation, survival, and migration of nervous cells of the system; positive regulator of angiogenesis; plays a role in the activation of both humoral and cellular immunity, including induction of DC maturation, functions, and interaction with T cells | 3.1 | + |
| NM_020377 | CYSLT2R | Cysteinyl leukotriene receptor 2 | Member of the superfamily of G protein-coupled receptors; mediates cell trafficking and innate immune responses; involved in the pathogenesis of chronic inflammatory diseases and allergic reactions | 2.9 | − |
| AI681260 | CD85 (LILRB1)† | Leukocyte immunoglobulin-like receptor B1 | ITIM-containing inhibitory leukocyte Ig-like receptor expressed on myeloid lineage cells and up-regulated during DC differentiation; binds MHCI; inhibitor of immune and inflammatory responses | 2.6 | + |
| NM_002346 | LY6 | Lymphocyte antigen 6 complex, locus E | Glycosylphosphatidylinositol-anchored molecule that belongs to the Ly-6 family, also known as stem cell antigen 2 (SCA2); type I interferon-inducible gene | 2.5 | − |
| M34986 | EPOR | Erythropoietin receptor | Member of the cytokine receptor family expressed on various hematopoietic cells with immunostimulatory activities | 2.1 | + |
| BE908995 | MYADM (SB135) | Myeloid-associated differentiation marker | Differentiation marker selectively expressed in myeloid cells, including DCs | 2.1 | + |
| GenBank accession no. . | Gene symbol . | Full name . | Main function(s) of gene product . | Fold increase‡ . | HRE§ . |
|---|---|---|---|---|---|
| Pattern recognition/scavenger receptors | |||||
| NM_005582 | CD180 (RP105)† | CD180 antigen | Belongs to the TLR family; negative regulator of TLR4 signaling on myeloid and B cells and of innate immune responses to pathogens and danger; NF-κB activator | 13.5 | − |
| NM_012072 | CD93 (C1qR1)† | Complement component 1q, receptor 1 | Defense collagen receptor for C1q, mannose-binding lectin, and surfactant protein A; mainly expressed on monocytes, neutrophils, and endothelial cells; modulates phagocytosis and clearance of apoptotic cells; mediates leukocyte-endothelial cell interactions | 8.6 | − |
| NM_003264 | TLR2† | Toll-like receptor 2 | Member of the TLR family expressed on cells of the innate immune system; plays an essential role in PAMP recognition leading to the generation of immune responses; implicated in the pathogenesis of autoimmune and inflammatory diseases | 7.7 | + |
| AW299226 | CD36† | Thrombospondin receptor | Multifunctional class B scavenger receptor; involved in the uptake of oxidized LDL, collagen, thrombospondin, anionic phospholipids, and apoptotic cells; adhesion molecule | 5 | + |
| BC005254 | CLEC2B† | C-type lectin domain family 2, member B | Member of the NK cell C-type lectin-like receptor family; platelet activation receptor; involved in tumor cell growth/metastatization | 4.8 | − |
| BE676623 | CD71† | Transferrin receptor | Regulator of cellular uptake of iron and iron metabolism; IgA receptor expressed on DCs | 4.5 | − |
| NM_001877 | CD21 (CR2)† | Complement component receptor 2 | EBV receptor overexpressed on B cells and follicular DCs; required for generation of immune responses to T cell antigens | 4.4 | − |
| AF285089 | CLEC2D† | C-type lectin domain family 2, member D | Member of the NK cell C-type lectin receptor family; pattern recognition receptor expressed on myeloid cells; mediates phagocytosis and proinflammatory cytokine production; regulates NK and APC cross-talk | 4.1 | + |
| NM_006770 | MARCO† | Macrophage receptor with collagenous structure | Member of the class A scavenger receptor family; pattern recognition receptor up-regulated on phagocytes in response to TLR agonists and Gram+/− bacteria; implicated in phagocytic activity and proinflammatory cytokine production; plays a role in host defense to pathogens, cell-cell recognition, and modified LDL scavenging | 3.7 | − |
| AL050262 | TLR1† | Toll-like receptor 1 | Member of the TLR family expressed on cells of the innate immune system; plays a fundamental role in PAMP recognition; mediates production of cytokines for the activation of innate immunity | 3.6 | + |
| AF313468 | CLEC7A | C-type lectin domain family 7, member A | Member of the C-type lectin-like receptor family; pattern recognition receptor for fungal β-glucan expressed on myeloid cells including DCs; activates phagocytosis, ROS and cytokine production, DC maturation, and T-cell costimulatory activity | 2.9 | + |
| NM_018690 | APOB48R | Apolipoprotein B48 receptor | Binds to apolipoprotein B48 on reticuloendothelial cells and regulates lipid transport into the cell | 2.9 | + |
| NM_000573 | CD35 (C3b/4bR1) | Complement component 3b/4b, receptor 1 | Member of the complement activation receptor (RCA) family expressed on follicular DCs and other leukocytes; mediates binding of complement-bound opsonins and immune complexes; enhances adaptive immune responses | 2.6 | − |
| U81501 | FPRL1 | Formyl peptide receptor-like 1 | High affinity receptor for bacteria-derived and host-derived N-formyl-methionyl peptides; key player in innate immunity and host defense against pathogen infection; mediates phagocyte chemotactic migration and IL-1 production | 2.6 | + |
| M74921 | EDNRB | Endothelin receptor type B | G protein-coupled receptor for the vasoactive peptide, endothelin; functions as a clearance receptor contributing to the regulation of vascular homeostasis | 2.2 | + |
| Costimulatory and adhesion/homing molecules | |||||
| 201666_at | TIMP1 | TIMP metallopeptidase inhibitor 1 | Matrix metalloproteinase inhibitor; promotes proliferation of several cell types and has an antiapoptotic function | 15.1 | + |
| NM_000889 | ITGB7 | Integrin, β 7 | Receptor for VCAM1 and fibronectin; interacts with MADCAM1 adhesion molecule expressed on vascular endothelium; plays a role in cell adhesive interactions | 8.3 | − |
| AK025016 | CD9† | CD9 antigen | Member of the tetraspanin family; facilitates the organization of multimolecular membrane complexes, including integrins; plays a role in leukocyte activation, differentiation, and migration | 8 | + |
| N95414 | ITGA2 (CD49B) | Integrin, α 2 | Receptor for laminin, collagen, fibronectin, and E-cadherin; involved in cell adhesion, cell surface-mediated signaling, extracellular matrix generation, and organization | 6.9 | + |
| AI653981 | L1CAM (CD171) | L1 cell adhesion molecule | Costimulatory/adhesion molecule belonging to the Ig superfamily; regulates DC transendothelial migration and trafficking; stimulates cancer cell motility and spreading | 6.7 | + |
| AF280547 | NRP1 | Neuropilin 1 | Coreceptor of tyrosine kinase receptors for vascular endothelial growth factor, class 3 semaphorins, and placenta growth factor; involved in the regulation of cell growth and migration, angiogenesis, DC and T-cell interaction, and in the initiation of primary immune responses | 5.8 | + |
| NM_001109 | ADAM8 (CD156) | ADAM metallopeptidase domain 8 | Membrane-anchored protein implicated in cell-cell and cell-matrix interactions | 4.5 | + |
| AF213460 | EPHA3 | Ephrin receptor A3 | Receptor for members of the ephrin-A family of receptor tyrosine kinases; mediates cell-cell contact, cellular adhesion, angiogenesis, and developmental remodeling | 4 | − |
| X74039 | CD87 | Plasminogen activator, urokinase receptor | Receptor for urokinase plasminogen activator; regulates cell-surface plasminogen activation; mediates cell adhesion by binding to vitronectin; modulates the proliferative, adhesive, and migratory cell phenotype | 3.8 | − |
| NM_013447 | CD312 (MR2) | EGF-like domain containing hormone receptor-like 2 | Member of the GPCR family of adhesion molecules differentially expressed during myeloid cell differentiation, maturation, and activation; plays a role in migration and adhesion | 3.8 | − |
| NM_000560 | CD53 | CD53 antigen | Member of the tetraspanin family; complexes with integrins and plays a role in the regulation of leukocyte activation, growth, and motility; contributes to the transduction of CD2-generated signals in T and NK cells | 3.6 | − |
| NM_002205 | ITGA5 (CD49) | Integrin, α 5 | Receptor for fibronectin and fibrinogen; involved in cell adhesion to fibronectin and cell surface mediated signaling | 3.6 | + |
| NM_002414 | CD99 | CD99 antigen | Mediates leukocyte adhesion amd transendothelial migration | 3.4 | + |
| BE870509 | MET (HGFR)† | Met proto-oncogene (hepatocyte growth factor receptor) | Widely expressed proto-oncogene encoding the tyrosine-kinase receptor for hepatocyte growth factor and scatter factor; plays a role in cell growth and motility in many physiologic and pathologic processes | 3.4 | + |
| M37780 | PECAM-1 (CD31)† | Platelet/endothelial cell adhesion molecule | Cell adhesion molecule predominantly expressed on endothelial cells | 3 | + |
| AW131039 | ITGB8 | Integrin, β 8 | Receptor for fibronectin; mediates cell-cell and cell-extracellular matrix interaction | 3 | + |
| NM_000655 | LECAM1 | Lymphocyte adhesion molecule 1 (selectin L) | Member of a family of adhesion/homing receptors required for leukocyte binding and rolling on endothelial cells; facilitates leukocyte migration into secondary lymphoid organs and inflammatory sites | 3 | − |
| M80436 | PAFR | Platelet-activating factor receptor | G protein-coupled receptor for platelet-activating factor with pleiotropic functions, including regulation of cell motility, smooth muscle contraction, cytokine synthesis, and release; implicated in allergy, asthma, septic shock, arterial thrombosis, and inflammatory processes | 2.8 | − |
| NM_001778 | CD48† | B-cell membrane protein | Activation-associated glycoprotein, member of the Ig superfamily; binds CD2 and regulates NK and T-cell activation | 2.7 | + |
| U94903 | CD44† | CD44 antigen | Receptor for hyaluronic acid, osteopontin, collagens, and matrix metalloproteinases; involved in cell-cell interaction, leukocyte adhesion, recirculation and homing, hematopoiesis, and tumor spreading | 2.7 | − |
| U87967 | CD39 | Ectonucleoside triphosphate diphosphohydrolase | Surface enzyme involved in the reduction of inflammatory cell adhesion and trafficking and tissue injury; contributes to vascular homeostasis regulation | 2.7 | − |
| D37781 | CD148† | Protein tyrosine phosphatase, receptor type, J | Receptor PTP induced in macrophages by proinflammatory stimuli; regulates cell spreading, cytoskeletal rearrangements, and chemotaxis | 2.3 | + |
| AJ277151 | CD134† | Tumor necrosis factor receptor superfamily, member 4 | Regulates T-cell expansion and survival, NK cell functions, and cross-talk with APCs; NF-κB activator | 2.1 | − |
| S78505 | PRLR | Prolactin receptor | Widespread receptor, major mediator of prolactin effects on cell proliferation, survival, cytoskeletal modification and differentiation | 2.1 | + |
| NM_002207 | ITGA9 | Integrin, α 9 | Receptor for VCAM1, tenascin, and osteopontin; participates in cell adhesion and motility | 2 | − |
| Immunoregulatory receptors | |||||
| U43677 | FCAR (CD89) | Fc fragment of IgA, receptor | ITAM-bearing receptor of the Fc region of IgA expressed on myeloid cells; mediates both pro- and anti-inflammatory functions of IgA | 55 | + |
| NM_018643 | TREM-1† | Triggering receptor expressed on myeloid cells 1 | Ig superfamily immunoregulatory receptor expressed on monocytes/macrophages and neutrophils; stimulates their proinflammatory functions and acts as an inflammatory amplifier via functional interaction with TLRs; involved in inflammatory diseases and septic shock | 39.8 | + |
| NM_001432 | EREG† | Epiregulin | Member of the epidermal growth factor (EGF) family; binds EGFR and members of ErbB tyrosine-kinase receptor family; mediates cell proliferation and proinflammatory cytokine production; promotes wound healing | 20.8 | + |
| NM_033438 | SLAMF9† | SLAM family member 9 | Member of the signaling leukocyte activation Ig subfamily; involved in innate and acquired immune responses through the regulation of multiple hematopoietic cell functions, including DC maturation | 12.2 | + |
| AW119113 | BDCA3 (CD141)† | Blood dendritic cell Ag (thrombomodulin) | Expressed on DCs; associated with TH2-skewed immune responses; plays a pathogenetic role in atopy and asthma | 11.4 | − |
| U90940 | FCGR2C (CD32) | Fc fragment of IgG, low affinity IIc, receptor | Involved in myeloid cell effector and regulatory functions, such as phagocytosis of immune complexes and modulation of antibody production by B cells | 11 | − |
| AI246590 | IRAK-2 | Interleukin-1 receptor-associated kinase 2 | IRAK kinase family member; key component of IL-1R and TLR signal transduction pathways; involved in the regulation of intracellular signaling networks controlling inflammation | 9.7 | + |
| NM_000570 | FCGR3A (CD16) | Fc fragment of IgG, low affinity IIIa, receptor | Type 1 transmembrane glycoprotein of the Ig superfamily expressed on several immune cells, including phagocytes; activating receptor involved in phagocytosis, inflammatory mediator release, ADCC, and immune complex clearance | 9.6 | + |
| AJ130712 | SIGLEC7 | Sialic acid binding Ig-like lectin 7 | ITIM-containing member of the CD33-related Siglec receptor family, weakly expressed on monocytic cells; acts as an inhibitory receptor | 8 | − |
| NM_006866 | LILRA2 (ILT1)† | Leukocyte immunoglobulin-like receptor A2 | Member of the leukocyte Ig-like immunoreceptor family, selectively expressed on myeloid cells, including DCs, and inflammatory tissues; acts as an activating receptor pairing with FcγR for signaling; promotes inflammatory responses | 6.4 | + |
| 1557444_at | TREML3† | Triggering receptor expressed on myeloid cells-like 3 | Ig superfamily immunoregulatory receptor expressed on myeloid cells, structurally related to the TREM gene family | 5.9 | − |
| AW241983 | CD307 | Fc receptor-like 5 | Differentiation antigen of B-lineage cells; plays an immunoregulatory role | 5 | − |
| NM_001772 | CD33 | CD33 antigen | Marker of myeloid cell differentiation belonging to the sialic acid-binding Ig-like lectin (Siglec) family; negative regulator of myeloid cell activation and DC generation | 4.7 | − |
| AK026133 | SEMA4B† | Semaphorin 4B | Integral membrane protein with an Ig extracellular domain, widely expressed throughout the nervous system; provides axonal guidance cues during neuronal development | 4.1 | − |
| NM_000956 | PTGER2 | Prostaglandin E receptor 2 | Receptor for prostaglandin E2, expressed on macrophages and mDCs; activates the expression of multiple genes implicated in the regulation of immunity and inflammation; involved in CCR7-mediated mDC migration | 3.7 | − |
| NM_004106 | FCER1G | Fc fragment of IgE, high affinity I, receptor | ITAM-containing subunit of the high-affinity IgE receptor and of other Fc receptors; expressed on mast cells and APCs; involved in allergic reactions mediated by IgE and in the regulation of immune responses | 3.6 | + |
| AF025529 | LILRA1 (LIR6) | Leukocyte immunoglobulin-like receptor A1 | Member of the leukocyte Ig-like immunoreceptor family expressed predominantly on monocytes/macrophages and B cells and at lower levels on DCs; associates with FcγR and acts as receptor for MHCI antigens | 3.5 | + |
| NM_006378 | SEMA4D (CD100)† | Semaphorin 4D | Integral membrane protein with an Ig extracellular domain; controls proliferation, survival, and migration of nervous cells of the system; positive regulator of angiogenesis; plays a role in the activation of both humoral and cellular immunity, including induction of DC maturation, functions, and interaction with T cells | 3.1 | + |
| NM_020377 | CYSLT2R | Cysteinyl leukotriene receptor 2 | Member of the superfamily of G protein-coupled receptors; mediates cell trafficking and innate immune responses; involved in the pathogenesis of chronic inflammatory diseases and allergic reactions | 2.9 | − |
| AI681260 | CD85 (LILRB1)† | Leukocyte immunoglobulin-like receptor B1 | ITIM-containing inhibitory leukocyte Ig-like receptor expressed on myeloid lineage cells and up-regulated during DC differentiation; binds MHCI; inhibitor of immune and inflammatory responses | 2.6 | + |
| NM_002346 | LY6 | Lymphocyte antigen 6 complex, locus E | Glycosylphosphatidylinositol-anchored molecule that belongs to the Ly-6 family, also known as stem cell antigen 2 (SCA2); type I interferon-inducible gene | 2.5 | − |
| M34986 | EPOR | Erythropoietin receptor | Member of the cytokine receptor family expressed on various hematopoietic cells with immunostimulatory activities | 2.1 | + |
| BE908995 | MYADM (SB135) | Myeloid-associated differentiation marker | Differentiation marker selectively expressed in myeloid cells, including DCs | 2.1 | + |
Gene expression profiling was carried out independently on the RNA purified from 3 independent mDC and H-mDC preparations, and comparative analysis of gene expression differences between the two experimental conditions was conducted as described in “Methods.” A GenBank accession number, a common gene symbol, a full name, a brief description of the gene product main functions, and the fold increase value are specified for each gene. Genes are ordered in each group by fold change.
Genes validated by quantitative RT-PCR.
The indicated values are calculated as the ratio of hypoxic/normoxic signals (mean of expression level of 3 experiments). Genes increased by ≥ 2-fold are shown.
The + sign indicates genes whose promoter contain members of the HRE family.