Transcript levels of the globin genes in the beta- and alpha-like globin clusters
| . | Cultured erythroblasts day 7 . | Cultured erythroblasts day 14 . | ||||
|---|---|---|---|---|---|---|
| Low-HbF (L) . | High-HbF (H) . | H/L ratio . | Low-HbF (L) . | High-HbF (H) . | H/L ratio . | |
| Beta-like globin cluster | ||||||
| ϵ-globin | 1.0 ± 0.6 × 10E3 | 2.7 ± 1.1 × 10E4* | 27 | 4.4 ± 1.5 × 10E2 | 7.9 ± 4.0 × 10E3* | 18 |
| γ-globin | 9.1 ± 4.1 × 10E5 | 1.2 ± 0.2 × 10E7* | 13 | 4.3 ± 2.7 × 10E5 | 6.0 ± 3.8 × 10E6* | 14 |
| δ-globin | 5.0 ± 4.4 × 10E5 | 1.9 ± 1.8 × 10E5* | 0.4 | 2.0 ± 2.0 × 10E5 | 7.4 ± 10.0 × 10E4* | 0.4 |
| β-globin | 1.3 ± 0.4 × 10E7 | 5.5 ± 1.8 × 10E6* | 0.4 | 1.5 ± 0.8 × 10E7 | 8.5 ± 4.9 × 10E6* | 0.6 |
| Total | 1.5 ± 0.5 × 10E7 | 1.8 ± 0.4 × 10E7 | 1.2 | 1.6 ± 0.8 × 10E7 | 1.5 ± 0.9 × 10E7 | 0.9 |
| Alpha-like globin cluster | ||||||
| ζ-globin | 6.9 ± 5.5 × 10E1 | 1.3 ± 0.8 × 10E4* | 190 | 2.7 ± 1.9 × 10E1 | 2.2 ± 2.4 × 10E2* | 8.1 |
| μ-globin | 4.0 ± 1.4 × 10E4 | 3.5 ± 1.4 × 10E4 | 0.9 | 1.4 ± 0.5 × 10E4 | 1.3 ± 1.0 × 10E4 | 0.9 |
| α-globin | 7.9 ± 2.0 × 10E6 | 8.7 ± 1.6 × 10E6 | 1.2 | 1.3 ± 0.6 × 10E7 | 1.1 ± 0.6 × 10E7 | 0.8 |
| θ-globin | 1.1 ± 0.5 × 10E3 | 1.4 ± 0.7 × 10E3 | 1.2 | 8.7 ± 2.9 × 10E2 | 5.5 ± 3.6 × 10E2 | 0.6 |
| Total | 7.9 ± 2.0 × 10E6 | 8.7 ± 1.6 × 10E6 | 1.1 | 1.3 ± 0.7 × 10E7 | 1.1 ± 0.7 × 10E7 | 0.8 |
| . | Cultured erythroblasts day 7 . | Cultured erythroblasts day 14 . | ||||
|---|---|---|---|---|---|---|
| Low-HbF (L) . | High-HbF (H) . | H/L ratio . | Low-HbF (L) . | High-HbF (H) . | H/L ratio . | |
| Beta-like globin cluster | ||||||
| ϵ-globin | 1.0 ± 0.6 × 10E3 | 2.7 ± 1.1 × 10E4* | 27 | 4.4 ± 1.5 × 10E2 | 7.9 ± 4.0 × 10E3* | 18 |
| γ-globin | 9.1 ± 4.1 × 10E5 | 1.2 ± 0.2 × 10E7* | 13 | 4.3 ± 2.7 × 10E5 | 6.0 ± 3.8 × 10E6* | 14 |
| δ-globin | 5.0 ± 4.4 × 10E5 | 1.9 ± 1.8 × 10E5* | 0.4 | 2.0 ± 2.0 × 10E5 | 7.4 ± 10.0 × 10E4* | 0.4 |
| β-globin | 1.3 ± 0.4 × 10E7 | 5.5 ± 1.8 × 10E6* | 0.4 | 1.5 ± 0.8 × 10E7 | 8.5 ± 4.9 × 10E6* | 0.6 |
| Total | 1.5 ± 0.5 × 10E7 | 1.8 ± 0.4 × 10E7 | 1.2 | 1.6 ± 0.8 × 10E7 | 1.5 ± 0.9 × 10E7 | 0.9 |
| Alpha-like globin cluster | ||||||
| ζ-globin | 6.9 ± 5.5 × 10E1 | 1.3 ± 0.8 × 10E4* | 190 | 2.7 ± 1.9 × 10E1 | 2.2 ± 2.4 × 10E2* | 8.1 |
| μ-globin | 4.0 ± 1.4 × 10E4 | 3.5 ± 1.4 × 10E4 | 0.9 | 1.4 ± 0.5 × 10E4 | 1.3 ± 1.0 × 10E4 | 0.9 |
| α-globin | 7.9 ± 2.0 × 10E6 | 8.7 ± 1.6 × 10E6 | 1.2 | 1.3 ± 0.6 × 10E7 | 1.1 ± 0.6 × 10E7 | 0.8 |
| θ-globin | 1.1 ± 0.5 × 10E3 | 1.4 ± 0.7 × 10E3 | 1.2 | 8.7 ± 2.9 × 10E2 | 5.5 ± 3.6 × 10E2 | 0.6 |
| Total | 7.9 ± 2.0 × 10E6 | 8.7 ± 1.6 × 10E6 | 1.1 | 1.3 ± 0.7 × 10E7 | 1.1 ± 0.7 × 10E7 | 0.8 |
Values are copies/ng total RNA. The globin copy number per nanogram of total RNA was determined by quantitative real-time PCR in adult human cultured erythroblasts on days 7 and 14. Information on PCR primers and probes is available in supplemental Table 1.
P < .05, comparing low-HbF (L) vs high-HbF (H) culture conditions (n = 15).