Presence of minor H antigen–specific CD8+ TCTL or minor H antigen–specific CD8+ TREG in healthy blood donors
| Donor . | Cytolytic T cells* . | tvDTH classification† . |
|---|---|---|
| ♀4 | No | Regulator (58%) |
| ♀5 | No | Regulator (60%) |
| ♀1 | No | Regulator (67%) |
| ♀3 | No | Regulator (67%) |
| ♀2 | No | Nonregulator (0%) |
| ♀6 | Yes | Nonregulator (0%) |
| ♀10 | Yes | Nonregulator (0%) |
| ♀7 | Yes | Nonregulator (10%) |
| ♀11 | Yes | Nonregulator (33%) |
| ♀9 | Yes | ND |
| ♂2 | No | Regulator (50%) |
| ♂4 | No | Regulator (50%) |
| ♂6 | No | Regulator (60%) |
| ♂3 | Yes | Regulator (75%) |
| ♂7 | ND | Regulator (75%) |
| ♂5 | Yes | Regulator (80%) |
| ♂1 | Yes | Nonregulator (0%) |
| Donor . | Cytolytic T cells* . | tvDTH classification† . |
|---|---|---|
| ♀4 | No | Regulator (58%) |
| ♀5 | No | Regulator (60%) |
| ♀1 | No | Regulator (67%) |
| ♀3 | No | Regulator (67%) |
| ♀2 | No | Nonregulator (0%) |
| ♀6 | Yes | Nonregulator (0%) |
| ♀10 | Yes | Nonregulator (0%) |
| ♀7 | Yes | Nonregulator (10%) |
| ♀11 | Yes | Nonregulator (33%) |
| ♀9 | Yes | ND |
| ♂2 | No | Regulator (50%) |
| ♂4 | No | Regulator (50%) |
| ♂6 | No | Regulator (60%) |
| ♂3 | Yes | Regulator (75%) |
| ♂7 | ND | Regulator (75%) |
| ♂5 | Yes | Regulator (80%) |
| ♂1 | Yes | Nonregulator (0%) |
ND indicates not determined.
Total tetramer-binding T cells were isolated by FACS sorting, polyclonally stimulated, and subsequently tested for cytolytic function.
The dominant presence of minor H alloantigen–specific Treg within total PBMCs was measured in the tvDTH assay.17 The percentages indicate the percentage inhibition of recall antigen–induced swelling footpad responses in the presence of the relevant minor H allo peptide.