Autosomal minor H antigen genotyping of 17 HLA-A2–sharing mother-offspring pairs included in the study
| Donor and relevant family . | Age, y . | HA-1 genotype* . | HA-2 genotype . | HA-8 genotype . |
|---|---|---|---|---|
| Mother 1†‡ | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 31 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 2† | 54 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 28 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 3† | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 33 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son | 27 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 4† | 72 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Son | 39 | HA-1 R/R | HA-2 V/V | HA-8 P/R |
| Son | 35 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Mother 5† | 53 | HA-1 H/H | HA-2 V/V | HA-8 P/R |
| Son | 24 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Son | 20 | HA-1 H/H | HA-2 V/V | HA-8 P/R |
| Mother 6† | 43 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Son | 13 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Mother 7† | 46 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Son | 15 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Son | 12 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Mother 8† | 43 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son | 9 | ND | ND | ND |
| Son | 7 | ND | ND | ND |
| Mother 9† | 40 | HA-1 H/R | HA-2 V/M | HA-8 P/P |
| Son | 6 | HA-1 H/R | HA-2 V/M | HA-8 P/P |
| Son | 4 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Mother 10† | 44 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Son | 20 | HA-1 H/R§ | HA-2 V/V | HA-8 P/P |
| Daughter | 17 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Daughter | 12 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| mGM | 74 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Mother 11† | 38 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Daughter | 6 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Mother 12 | 72 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son 1† | 46 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 13 | 58 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Son 2† | 34 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Mother 14 | 59 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Son 3† | 34 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Mother 15 | 73 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son 4† | 44 | HA-1 R/R | HA-2 M/M | HA-8 P/P |
| Mother 16‡ | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son 5† | 31 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 17 | 45 | HA-1 R/R | HA-2 V/M | HA-8 P/P |
| Son 6† | 21 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 18 | 51 | HA-1 H/R | ND | HA-8 R/P |
| Son 7† | 19 | HA-1 H/R | ND | HA-8 P/P |
| Donor and relevant family . | Age, y . | HA-1 genotype* . | HA-2 genotype . | HA-8 genotype . |
|---|---|---|---|---|
| Mother 1†‡ | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 31 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 2† | 54 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 28 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 3† | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son | 33 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son | 27 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 4† | 72 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Son | 39 | HA-1 R/R | HA-2 V/V | HA-8 P/R |
| Son | 35 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Mother 5† | 53 | HA-1 H/H | HA-2 V/V | HA-8 P/R |
| Son | 24 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Son | 20 | HA-1 H/H | HA-2 V/V | HA-8 P/R |
| Mother 6† | 43 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Son | 13 | HA-1 H/H | HA-2 V/V | HA-8 P/P |
| Mother 7† | 46 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Son | 15 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Son | 12 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Mother 8† | 43 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son | 9 | ND | ND | ND |
| Son | 7 | ND | ND | ND |
| Mother 9† | 40 | HA-1 H/R | HA-2 V/M | HA-8 P/P |
| Son | 6 | HA-1 H/R | HA-2 V/M | HA-8 P/P |
| Son | 4 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Mother 10† | 44 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Son | 20 | HA-1 H/R§ | HA-2 V/V | HA-8 P/P |
| Daughter | 17 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Daughter | 12 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| mGM | 74 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Mother 11† | 38 | HA-1 R/R | HA-2 V/V | HA-8 P/P |
| Daughter | 6 | HA-1 H/R | HA-2 V/V | HA-8 P/P |
| Mother 12 | 72 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son 1† | 46 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 13 | 58 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Son 2† | 34 | HA-1 R/R | HA-2 V/M | HA-8 P/R |
| Mother 14 | 59 | HA-1 H/R | HA-2 V/V | HA-8 P/R |
| Son 3† | 34 | HA-1 R/R | HA-2 V/V | HA-8 R/R |
| Mother 15 | 73 | HA-1 H/R | HA-2 V/M | HA-8 P/R |
| Son 4† | 44 | HA-1 R/R | HA-2 M/M | HA-8 P/P |
| Mother 16‡ | 57 | HA-1 H/R | HA-2 M/M | HA-8 R/R |
| Son 5† | 31 | HA-1 R/R | HA-2 V/M | HA-8 R/R |
| Mother 17 | 45 | HA-1 R/R | HA-2 V/M | HA-8 P/P |
| Son 6† | 21 | HA-1 R/R | HA-2 M/M | HA-8 P/R |
| Mother 18 | 51 | HA-1 H/R | ND | HA-8 R/P |
| Son 7† | 19 | HA-1 H/R | ND | HA-8 P/P |
ND indicates not determined; and mGM, HLA-A2+ maternal grandmother.
Autosomal minor H antigens are encoded by diallelic loci composed of an immunogenic allele, for example, HA-1H, HA-2V, and HA-8R, and a nonimmunogenic or a “null” allele, for example, HA-1R, HA-2M, and HA-8P. Consequently, persons expressing HA-1H/R are referred to as HA-1+, whereas HA-1R/R persons are designated as HA-1−.
Subjects analyzed in the study.
The same pair.
The autosomal minor H alloantigen under study is indicated in bold. HY is studied in female donors in case no autosomal minor H mismatch exists between the mother and her male offspring.